Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,570	C→T	R55R (CGG→CGA)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,570	0	C	T	100.0%	113.5 / NA	37	R55R (CGG→CGA)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

CGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCT  >  NC_000913/1829435‑1829697                                                                                                                                        |                                                                                                                                cGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTcgcg                                                                                                                             >  2:521395/1‑140 (MQ=255)                            gcgcGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTg                                                                                                  <  2:339667/140‑1 (MQ=255)                                ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                              >  1:281124/1‑140 (MQ=255)                                ggCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCg                                                                                              >  1:608175/1‑140 (MQ=255)                                  cTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGTCCGAc                                                                                            >  1:490676/1‑140 (MQ=255)                                    tACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACaa                                                                                          <  1:202022/140‑1 (MQ=255)                                     aCCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAg                                                                                         <  2:12419/140‑1 (MQ=255)                                          tttCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGc                                                                                    <  1:521395/140‑1 (MQ=255)                                                tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAg                                                                                         >  2:680691/1‑129 (MQ=255)                                                tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAg                                                                                         <  1:680691/129‑1 (MQ=255)                                                tGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGAcc                                                                              >  1:188941/1‑140 (MQ=255)                                                    aataatCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAg                                                                          >  2:617884/1‑140 (MQ=255)                                                            cgGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcg                                                                  >  2:753254/1‑140 (MQ=255)                                                              gTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                >  1:524739/1‑140 (MQ=255)                                                              gTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGcggcatcggc                                                                >  1:405939/1‑140 (MQ=255)                                                                      ggCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgc                                                        <  2:405939/140‑1 (MQ=255)                                                                        cTTTATTGTTTTCCAGCTGTGCGGCAAAGCTGTCGCCAATGGCATGACCTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                      <  2:382110/140‑1 (MQ=255)                                                                        cTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAACTGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGTCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcct                                                      <  2:490676/140‑1 (MQ=255)                                                                         tttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgcctt                                                     <  1:133875/140‑1 (MQ=255)                                                                          ttATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttg                                                    <  1:617884/140‑1 (MQ=255)                                                                            aTTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCAttgccttgcc                                                  >  2:802028/1‑140 (MQ=255)                                                                              tGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGACATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCAt                                                <  2:281124/140‑1 (MQ=255)                                                                               gCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATa                                               <  2:567061/140‑1 (MQ=255)                                                                                  ttCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTAGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAaca                                            >  2:58781/1‑140 (MQ=255)                                                                                       gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                                       >  1:486009/1‑140 (MQ=255)                                                                                       gcagTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCg                                       >  2:567373/1‑140 (MQ=255)                                                                                         agTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGcc                                     <  1:444380/140‑1 (MQ=255)                                                                                         agTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGcc                                     <  1:512671/140‑1 (MQ=255)                                                                                          gTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGccc                                    <  2:486009/140‑1 (MQ=255)                                                                                           tGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCAGCGGCATCATTGCCTTGCCATAACACCTCGCCCg                                   >  2:363655/1‑140 (MQ=255)                                                                                             cGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGAt                                 >  1:561801/1‑140 (MQ=255)                                                                                             cGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCACCGGAATCATTGCGTTGCGTTAACACCTCGCCCGAt                                 >  2:400845/1‑140 (MQ=255)                                                                                                                  cATGACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCCTAACACCGCGCCCGGTACCGGGTTACGCTTCACAcgc            >  1:363819/1‑140 (MQ=255)                                                                                                                     gACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt         <  2:561801/140‑1 (MQ=255)                                                                                                                     gACGTTCAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGAt         <  2:526950/140‑1 (MQ=255)                                                                                                                          tCAGCAAAGGAAAGTCGCGCCCGGCGCGGAACCGCAGCATGGGCTGCCGGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGcc    <  2:363819/140‑1 (MQ=255)                                                                                                                           cAGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGccc   >  2:810376/1‑140 (MQ=255)                                                                                                                            aGCAAATGAAAGTCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCt  <  1:58781/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                CGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTCTGGCAATAATCGCGGTTAATTCGGCTTTATTGCTTTCCAGCAGTGCGGCAAAGCGTTCGACAACGGCATGACGTTCAGCAAATGAAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCT  >  NC_000913/1829435‑1829697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑