| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 250,303 | A→G | pseudogene (232/756 nt) | lafU → | flagellar system protein, promoterless fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 250,303 | 0 | A | G | 96.5% | 90.3 / NA | 29 | pseudogene (232/756 nt) | lafU | flagellar system protein, promoterless fragment |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (12/16); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CCGCCAAAACCTGCCACCGTGGCGGTGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACACCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCGGCAGCGCGAAGATTATGCCGTTCT > NC_000913/250174‑250435 | ccGCCAAAACCTGCCACCGTGGCGGTGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCg > 1:717233/1‑140 (MQ=255) aaCCTGCCACCGTGGCGGTGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATgcg > 2:574467/1‑140 (MQ=255) tGCCACCGTGGCGGTGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCata < 1:574467/140‑1 (MQ=255) cGTGGCGGTGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAg < 1:445562/140‑1 (MQ=255) tGGCGGGGCCGGAAGAGACGGAGTAAAAAGCGCGTGATGTGAATGAGAAAACGGGGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGATCTGGCAACCAGTTTCATCGCCATTGCCCGTGATGGGCATATGGAAGCg > 2:276178/1‑140 (MQ=255) ggTGCCGGAAGAGACTGAGAAATAACCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCTCCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATc < 1:277174/140‑1 (MQ=255) ggTGCCGGAAGAGACGGAGAAAAAACCGCTTGATGTGGATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATc < 1:361573/140‑1 (MQ=255) tGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCtg > 2:774759/1‑140 (MQ=255) aaGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCtggagatg < 1:774759/140‑1 (MQ=255) aaaGCGCGTGATGTGAATGAGACAACGGCGCTGCTCAAGGAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCa < 2:209364/140‑1 (MQ=255) aaaGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCa < 2:808891/140‑1 (MQ=255) gcgcGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAggg < 2:13955/140‑1 (MQ=255) tgAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTg < 1:505280/140‑1 (MQ=255) tgAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTg < 2:717233/140‑1 (MQ=255) aaaaCGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTaa > 1:82781/1‑140 (MQ=255) cgcTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTTTTCGCCAGGGATAACGCTTGCTGATTAAAgacgac > 2:216555/1‑140 (MQ=255) cgcTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAgacgac < 1:559199/140‑1 (MQ=255) cgcTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAgacgac < 2:82781/140‑1 (MQ=255) gaaATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAgacgac > 1:170677/1‑127 (MQ=255) gaaATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAgacgac < 2:170677/127‑1 (MQ=255) gaaATCCGCCACCGAGCTTGGTCAGGTGGCAACCAGTCTCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAata < 1:276178/140‑1 (MQ=255) aaTCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATg > 1:682441/1‑140 (MQ=255) aaTCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATg > 2:51136/1‑140 (MQ=255) aTCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGt < 2:682441/140‑1 (MQ=255) gAGCTTGGTGAGCTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCgg < 2:461864/140‑1 (MQ=255) tGAGCTGGCAACCAGCATCAACACCCTTGACCGTGATACGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCGGCAGCGCGa < 1:216555/140‑1 (MQ=255) cTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACGAGAACCGTAATGTGTTTGAACACGGCAGCGCGAAGAt > 1:259662/1‑140 (MQ=255) cTGGCAACCAGTATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCGGCAGCGCGAAGAt > 1:820200/1‑140 (MQ=255) tATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCGGCAGCGCGAAGATTATGCCGTTCt > 1:545383/1‑140 (MQ=255) tATCAACGCCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCGGCAGCGCGAAGATTATGCCGTTCt > 1:5213/1‑140 (MQ=255) | CCGCCAAAACCTGCCACCGTGGCGGTGCCGGAAGAGACGGAGAAAAAAGCGCGTGATGTGAATGAGAAAACGGCGCTGCTGAAGAAGAAATCCGCCACCGAGCTTGGTGAGCTGGCAACCAGTATCAACACCATTGCCCGTGATGCGCATATGGAAGCGAATCTGGAGATGGAGATTGTTCCCCAGGGATTACGCGTGCTGATTAAAGACGACCAGAACCGCAATATGTTTGAACGCGGCAGCGCGAAGATTATGCCGTTCT > NC_000913/250174‑250435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |