| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,863,508 | T→C | R246R (CGT→CGC) | gapA → | glyceraldehyde‑3‑phosphate dehydrogenase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,863,508 | 0 | T | C | 97.9% | 167.3 / NA | 48 | R246R (CGT→CGC) | gapA | glyceraldehyde‑3‑phosphate dehydrogenase A |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (30/17); minor base G (1/0); total (31/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTTGCACT > NC_000913/1863382‑1863640 | catcCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTg > 1:637568/1‑140 (MQ=255) cGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACt > 1:771760/1‑140 (MQ=255) cGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACt > 1:701491/1‑140 (MQ=255) ccTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTAc > 1:83142/1‑140 (MQ=255) ccTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTAc > 1:614382/1‑140 (MQ=255) ctACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAg < 2:781260/140‑1 (MQ=255) ctACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGATATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAg < 1:138925/140‑1 (MQ=255) tACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACtt < 1:633466/134‑1 (MQ=255) tACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACtt > 2:633466/1‑134 (MQ=255) gctAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGTCCTGACCGGTCGGCTGGAAAAAGCTGCAACGTACGAGCAGATCAAAGc > 2:209402/1‑140 (MQ=255) gctAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGc > 1:620746/1‑140 (MQ=255) aGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAg > 2:532078/1‑140 (MQ=255) aGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAg < 1:487205/140‑1 (MQ=255) tAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAgctg < 1:587292/140‑1 (MQ=255) tGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGaa > 1:134446/1‑140 (MQ=255) cTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAAt < 1:25060/140‑1 (MQ=255) gAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGa > 2:154203/1‑140 (MQ=255) aaTGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGaa > 2:585119/1‑140 (MQ=255) tGGCAAACTGACTGGTATGGCGTTCTGCGATCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAg < 1:222584/140‑1 (MQ=255) aaCTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGtt < 2:119507/140‑1 (MQ=255) aaCTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAAATCAAAGCAGCCGTTAGAGCTGCTGGTGAAAGCGAAATGAAAGGCGtt > 2:222584/1‑140 (MQ=255) tgactgGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATTAAAGCTGCCGTTAATGCTGCTGCTGAAGGCCGAATGAAAGGCGTTCTg > 2:358575/1‑140 (MQ=255) tgactgGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGtt > 1:578669/1‑137 (MQ=255) tgactgGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGtt < 2:578669/137‑1 (MQ=255) aTGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGtt > 1:496081/1‑129 (MQ=255) aTGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGtt < 2:496081/129‑1 (MQ=255) gcgttccgcgttccGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGa > 2:684103/1‑140 (MQ=255) gttccgcgttccgACACCGAACGTACCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAg < 1:358575/140‑1 (MQ=255) ttccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGa > 1:704806/1‑140 (MQ=255) ttccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGa < 2:614382/140‑1 (MQ=255) ttccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGa < 1:585119/140‑1 (MQ=255) ttccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGATGATGGCAAAATGAAAAGCGTTCTGGGCTACACCGAAGa > 2:285312/1‑140 (MQ=255) ccgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATg > 2:24315/1‑140 (MQ=255) cgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAATGCGAAATGAAAGGCGTTCTGGTCTACACCGAAGATGa > 1:214516/1‑140 (MQ=255) cgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAATGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGa > 1:368268/1‑140 (MQ=255) cgcgttccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGa > 1:446688/1‑140 (MQ=255) ccgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACgtagt < 1:532078/140‑1 (MQ=255) cgACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACgtagta > 1:408516/1‑140 (MQ=255) aTCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCa < 2:83142/140‑1 (MQ=255) aTCTGTAGTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTATGGGCTAAACCGAAGAGGACGTCGTATCTACCGATTTCa > 2:384671/1‑140 (MQ=255) tAGTTGACCTGACCGATCGCCTCGATAAAACTGCAACCTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGc < 1:384671/140‑1 (MQ=255) gTTGACCTGACCGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGa > 1:138921/1‑140 (MQ=255) gacctgaccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGt > 2:29620/1‑140 (MQ=255) gacctgaccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGt > 1:737644/1‑140 (MQ=255) gacctgaccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCGGGGCTACAGCGAGGATCACGTAGTAGCTACCGATTTCAACGGCGAAGt > 2:302787/1‑140 (MQ=255) gaccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTTGCa > 1:559863/1‑140 (MQ=255) gaccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTTGCa < 1:29620/140‑1 (MQ=255) ccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTTGCACt < 2:134446/140‑1 (MQ=255) ccGTTCGCCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTTGCACt > 2:675206/1‑140 (MQ=255) | CATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGACCTGACCGTTCGTCTGGAAAAAGCTGCAACTTACGAGCAGATCAAAGCTGCCGTTAAAGCTGCTGCTGAAGGCGAAATGAAAGGCGTTCTGGGCTACACCGAAGATGACGTAGTATCTACCGATTTCAACGGCGAAGTTTGCACT > NC_000913/1863382‑1863640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |