Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,785	T→C	V145A (GTG→GCG)  P264P (CCA→CCG)	yeaL → nimR ←	conserved inner membrane protein YeaL DNA‑binding transcriptional repressor NimR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,785	0	T	C	97.9%	157.0 / NA	47	V145A (GTG→GCG)  P264P (CCA→CCG)	yeaL nimR	conserved inner membrane protein YeaL DNA‑binding transcriptional repressor NimR
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (14/32);  total (14/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACT  >  NC_000913/1874653‑1874920                                                                                                                                     |                                                                                                                                        gcggcgTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGaaa                                                                                                                                  <  1:537324/140‑1 (MQ=255)   ggcgTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACa                                                                                                                                <  1:683060/140‑1 (MQ=255)           gTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTaa                                                                                                                        <  1:71280/140‑1 (MQ=255)            ttAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAAt                                                                                                                       <  1:668813/140‑1 (MQ=255)              aaTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGaaa                                                                                                                                  <  1:207499/127‑1 (MQ=255)              aaTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGaaa                                                                                                                                  >  2:207499/1‑127 (MQ=255)               aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAAtctc                                                                                                                    >  2:142358/1‑140 (MQ=255)               aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTATGGTTTCGCTGTTTCCGGGGACACAGTAGTTAAtctc                                                                                                                    >  1:374818/1‑140 (MQ=255)                  ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCa                                                                                                                 >  2:765044/1‑140 (MQ=255)                    cagccagcCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAat                                                                                                               >  2:456867/1‑140 (MQ=255)                          gccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCg                                                                                                         >  2:56029/1‑140 (MQ=255)                           ccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCgg                                                                                                        <  1:56029/140‑1 (MQ=255)                               aaCTGGTCGCCGGGCTGCTGGTGGGCACTGTTTTATGCGTAGCGCTGTCTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTg                                                                                                    <  2:306862/140‑1 (MQ=255)                                    gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                               <  1:142358/140‑1 (MQ=255)                                    gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                               <  1:446253/140‑1 (MQ=255)                                    gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                               >  1:297770/1‑140 (MQ=255)                                    gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAATTAGTCTCGCAATTTATCGGCCTGGCGtt                                                                                               >  1:289970/1‑140 (MQ=255)                                         cGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                                          >  1:186863/1‑140 (MQ=255)                                              tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                     <  2:297770/140‑1 (MQ=255)                                              tgctgGTAGGCACTGTTTTAGGAGTAGCGCTTTTTTGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                     <  2:289970/140‑1 (MQ=255)                                                ctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt                                                                                   <  2:55419/140‑1 (MQ=255)                                                      ggCTCTGTTTAAGGCGTTGCGCTGTTTCGCGACATACCGGTTGGGCCGTTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                                             <  2:374818/140‑1 (MQ=255)                                                       gCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCT‑GTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                           <  1:205645/140‑1 (MQ=255)                                                        cACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                           <  2:186863/140‑1 (MQ=255)                                                          cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                         <  2:617536/140‑1 (MQ=255)                                                          cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                         <  1:793040/140‑1 (MQ=255)                                                          cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                         <  1:792076/140‑1 (MQ=255)                                                          cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                         <  1:765044/140‑1 (MQ=255)                                                             ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                           >  2:555274/1‑135 (MQ=255)                                                             ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                           <  1:555274/135‑1 (MQ=255)                                                                      tAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAgg                                                             <  2:431792/140‑1 (MQ=255)                                                                              tttCGCGGCGTACCGCTTGGGCCGCGTATCGCCGCGGTTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgtt                                                     <  1:391587/140‑1 (MQ=255)                                                                                 cgcgGCGTACCGGTTGGGCCGCCTAGTGCTGCGGGTCAGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                                                  <  1:246309/140‑1 (MQ=255)                                                                                        tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcc                                           >  1:749995/1‑140 (MQ=255)                                                                                          ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcccc                                         <  1:52697/140‑1 (MQ=255)                                                                                                gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTa                                   >  1:137188/1‑140 (MQ=255)                                                                                                 ggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTAt                                  >  1:555479/1‑140 (MQ=255)                                                                                                       ttATTGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAGACATAGTAATAAGGGCCGTTGTTGAATCATACCCCAGCGTAAGAGCaa                            >  2:340473/1‑140 (MQ=255)                                                                                                        tATTGCCGCGGGTCTGGTTTCGTTGATTGCGGGGATACAGTAGTTAATATCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc                           <  2:351341/140‑1 (MQ=255)                                                                                                           tGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt                        <  1:772531/140‑1 (MQ=255)                                                                                                           tGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt                        >  2:469952/1‑140 (MQ=255)                                                                                                           tGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt                        <  2:555479/140‑1 (MQ=255)                                                                                                           tGCCGCGGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt                        <  2:749995/140‑1 (MQ=255)                                                                                                           tGCCGCGGGTCTGGTATCGCTGCTTACGGGGAAACAGGAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACttt                        <  1:340473/140‑1 (MQ=255)                                                                                                               gcgGGTCTGGTTTCGCTTATTGTGGGGAAACCGTACTTAATCTCGCAAGATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGt                    <  2:248553/140‑1 (MQ=255)                                                                                                                cgGGTCTGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTa                   <  1:456867/140‑1 (MQ=255)                                                                                                                      tGGTTTCGCTGATTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTAt             <  1:485591/140‑1 (MQ=255)                                                                                                                                 aTTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACt  <  1:444555/140‑1 (MQ=255)                                                                                                                                 aTTGCGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACt  <  1:469952/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GCGGCGTGACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACT  >  NC_000913/1874653‑1874920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑