Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,630	A→C	V319G (GTC→GGC)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,630	0	A	C	100.0%	99.2 / NA	31	V319G (GTC→GGC)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/14);  total (17/14)

CTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGA  >  NC_000913/1900509‑1900759                                                                                                                          |                                                                                                                                  ctctTCCAGCGCCACCACCAGTTCTTTAGCACGTACAATCCCGATGATATCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCaa                                                                                                                 <  2:292672/140‑1 (MQ=255)   ctTCCAGCGCCACCAGCAGTTCTTTTGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAAcc                                                                                                               <  1:327090/140‑1 (MQ=255)    ttCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGTTCGCGGATTTCGTCCCCCCCGCGATTAGCGTCAAccc                                                                                                              >  1:270078/1‑140 (MQ=255)     tCCAGCGCCACCAGCAGTTCTTTAGAACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCa                                                                                                             <  1:301038/140‑1 (MQ=255)         gcgcCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCtt                                                                                                         >  1:826643/1‑140 (MQ=255)              accaGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCC‑‑GATTAGCGTCAACCCAGCTTATTTCAc                                                                                                  >  1:94783/1‑140 (MQ=255)                       tCTTTAGCACGTACAATCCCGATGATTTCATACAGCTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCATTTGCTCGCGGGTTGCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCgcgg                                                                                           <  2:270078/140‑1 (MQ=255)                                cGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGAt                                                                                  >  2:347900/1‑140 (MQ=255)                                       tACCGATGATTTCATCCAGTTCTCCGCGACAGACCCGGACCAGACTGTACGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCgcgc                                                                           <  2:347239/140‑1 (MQ=255)                                            atgatTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAgaga                                                                      >  2:679288/1‑140 (MQ=255)                                            atgatTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAgaga                                                                      >  2:180/1‑140 (MQ=255)                                            atgatTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGATTATTTCACCGAGCGGCGTCATGATCCCGCGCAgaga                                                                      >  1:347239/1‑140 (MQ=255)                                               atTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAAcg                                                                   >  2:575623/1‑140 (MQ=255)                                               atTTCATCCAGTTCAACGCGACCTATCGGTAACAGACAGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAAcg                                                                   <  1:218710/140‑1 (MQ=255)                                                               cgcgACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGt                                                   >  1:423665/1‑140 (MQ=255)                                                                gcgACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTa                                                  <  2:826643/140‑1 (MQ=255)                                                                    cATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGcc                                              <  1:724849/140‑1 (MQ=255)                                                                         cGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTaa                                         <  2:661148/140‑1 (MQ=255)                                                                                  aCTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGACAGGGTCAGTACGCCGTTAATCATGTAAc                                >  1:307273/1‑140 (MQ=255)                                                                                      tgCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGttc                            >  1:722464/1‑140 (MQ=255)                                                                                      tgCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCCCAGAGAACACGAGGCCAGCGTCAGTACGCCCTTAATCATGTAACGttc                            >  2:283099/1‑140 (MQ=255)                                                                                            gAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGt                                   <  1:179046/127‑1 (MQ=255)                                                                                            gAAGAGAGCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGt                                   >  2:179046/1‑127 (MQ=255)                                                                                                agagCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGTGACGCCAGAGTCAGTAAGCCGTTAAGCATGTAACGTTCTTCTTCGGCa                  >  2:227168/1‑140 (MQ=255)                                                                                                agagCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCa                  >  1:764651/1‑140 (MQ=255)                                                                                                agagCAGTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGGCAGTACGACGTAAACCATGTAACGTTCTTCTTCGGCa                  >  1:266293/1‑140 (MQ=255)                                                                                                  agCAGTTGCTCGCGGATTTCATCGCCCCC‑‑GATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATg              <  2:94783/140‑1 (MQ=255)                                                                                                      gTTGCTCGCGGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGACAAATGCa            <  1:180/140‑1 (MQ=255)                                                                                                              cgGATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCgg    >  1:632021/1‑140 (MQ=255)                                                                                                               ggATTTCATCGCCCCCGAGATTCGCGTCAACCCAGCTTATTTCACCGCCCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCggg   <  2:231030/140‑1 (MQ=255)                                                                                                                gATTTCATCGCCCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGa  <  2:423665/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                  CTCTTCCAGCGCCACCAGCAGTTCTTTAGCACGTACAATACCGATGATTTCATCCAGTTCACCGCGACATACCGGGAACAGACTGTGCGGTGAAGAGAGCAGTTGCTCGCGGATTTCATCGACCCCGAGATTAGCGTCAACCCAGCTTATTTCACCGCGCGGCGTCATGATCCCGCGCAGAGAACGCGACGCCAGCGTCAGTACGCCGTTAATCATGTAACGTTCTTCTTCGGCAAATGCACCTTCCGGGA  >  NC_000913/1900509‑1900759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑