Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,825	T→C	intergenic (‑234/+436)	yobF ← / ← yebO	DUF2527 domain‑containing protein YobF/uncharacterized protein YebO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,825	0	T	C	100.0%	277.4 / NA	80	intergenic (‑234/+436)	yobF/yebO	DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/45);  total (35/45)

ATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAA  >  NC_000913/1907694‑1907961                                                                                                                                    |                                                                                                                                         aTACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGcc                                                                                                                                  >  1:578433/1‑140 (MQ=255) aTACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGcc                                                                                                                                  <  1:590936/140‑1 (MQ=255)     gTCTTCCACTTTTTTCGCACACCTACGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTg                                                                                                                              <  1:390724/140‑1 (MQ=255)        ttCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGga                                                                                                                           >  1:622857/1‑140 (MQ=255)         tCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgag                                                                                                                          <  2:648687/140‑1 (MQ=255)         tCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgag                                                                                                                          <  2:13600/140‑1 (MQ=255)          ccACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         >  2:745611/1‑140 (MQ=255)          ccACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         <  2:110092/140‑1 (MQ=255)          ccACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         <  1:492071/140‑1 (MQ=255)          ccACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         <  2:707460/140‑1 (MQ=255)          ccACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         <  1:154976/140‑1 (MQ=255)          ccACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         <  1:87997/140‑1 (MQ=255)          ccACTTTTTTTGCACACTTATGAAATGGAATACGCGCTGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGgaga                                                                                                                         <  1:391438/140‑1 (MQ=255)            aCTTTTTTTGCACTCTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAGCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAg                                                                                                                       <  1:453931/140‑1 (MQ=255)            aCTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAg                                                                                                                       <  2:118709/140‑1 (MQ=255)            aCTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAg                                                                                                                       <  1:58169/140‑1 (MQ=255)            aCTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAg                                                                                                                       <  1:598831/140‑1 (MQ=255)             cTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGt                                                                                                                      >  1:721278/1‑140 (MQ=255)                        cacTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAgt                                                                                                           <  1:745611/140‑1 (MQ=255)                                    ggAATACGCGCCGATATATCATTGTTCTTTACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATc                                                                                               <  1:271742/140‑1 (MQ=255)                                     gagtccgcgGCGATATATCCTTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGCTCAATTGCTCCAGGCCAGGGTGAGAAGTTGTTACAGAGTGCGTTCAATATca                                                                                              >  2:401169/6‑140 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTTTTACATAGTGCGTGCAATATca                                                                                              >  1:320605/1‑140 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAgtgcgtgc                                                                                                     >  2:584104/1‑133 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAgtgcgtgc                                                                                                     <  1:584104/133‑1 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATca                                                                                              >  1:616107/1‑140 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATca                                                                                              >  1:134588/1‑140 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATca                                                                                              >  1:519242/1‑140 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATca                                                                                              >  1:507410/1‑140 (MQ=255)                                     gAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATca                                                                                              >  1:20926/1‑140 (MQ=255)                                         aCGCGCCGATATATCATTGTGCTTAACCTTGTCAGTTCATGCAGATACGTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACAtt                                                                                          <  2:320605/140‑1 (MQ=255)                                              ccGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTg                                                                                     <  2:622857/140‑1 (MQ=255)                                              ccGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTg                                                                                     <  2:626015/140‑1 (MQ=255)                                                         ttGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGATAAGTTATTCCATCGTGCGTGCAATAACACAATTTTTGCTATGCAATGa                                                                          >  2:338876/1‑140 (MQ=255)                                                         ttGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGa                                                                          >  1:345987/1‑140 (MQ=255)                                                           gtgCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAAt                                                                        >  2:710888/1‑140 (MQ=255)                                                           gtgCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAAt                                                                        >  1:124266/1‑140 (MQ=255)                                                            tgCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATa                                                                       >  2:781618/1‑140 (MQ=255)                                                              cTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATaaa                                                                     >  1:414604/1‑140 (MQ=255)                                                              cTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATaaa                                                                     >  2:730692/1‑140 (MQ=255)                                                               ttAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATaaaa                                                                    <  1:781618/140‑1 (MQ=255)                                                                 aaCCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAg                                                                  >  2:95840/1‑140 (MQ=255)                                                                    cTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTta                                                               <  2:578433/140‑1 (MQ=255)                                                                      tGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTTTGCaa                                                                             <  2:819017/124‑1 (MQ=255)                                                                      tGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCaa                                                                             >  1:819017/1‑124 (MQ=255)                                                                      tGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTtata                                                             >  1:542760/1‑140 (MQ=255)                                                                      tGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCGATGCAATGAATAAAAAGTtata                                                             >  1:348461/1‑140 (MQ=255)                                                                        ccAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTGTTGATATGCAATGAATAAAAAGTTATATc                                                           >  1:303593/1‑140 (MQ=255)                                                                         cAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCa                                                          <  2:507410/140‑1 (MQ=255)                                                                            ttCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACtt                                                       >  2:498873/1‑140 (MQ=255)                                                                              cAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACtttt                                                     <  2:134588/140‑1 (MQ=255)                                                                              cAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGATCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACtttt                                                     <  1:365882/140‑1 (MQ=255)                                                                                        cTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATaaaa                                           <  1:710888/140‑1 (MQ=255)                                                                                           aaaCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAAcag                                        >  2:496887/1‑140 (MQ=255)                                                                                            aaCTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAAcagt                                       <  2:124266/140‑1 (MQ=255)                                                                                                 gCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGtt                                  >  2:719586/1‑140 (MQ=255)                                                                                                   gTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTaa                                <  2:616107/140‑1 (MQ=255)                                                                                                   gTATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTaa                                <  1:730692/140‑1 (MQ=255)                                                                                                    tATTTTCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAAc                               <  2:414604/140‑1 (MQ=255)                                                                                                      ttttCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                             <  1:719586/140‑1 (MQ=255)                                                                                                        ttCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCt                           <  2:422967/140‑1 (MQ=255)                                                                                                        ttCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCt                           <  2:649244/140‑1 (MQ=255)                                                                                                        ttCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCt                           <  1:95840/140‑1 (MQ=255)                                                                                                        ttCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCt                           <  2:519242/140‑1 (MQ=255)                                                                                                        ttCTAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCt                           <  2:542760/140‑1 (MQ=255)                                                                                                           tAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                             >  2:775219/1‑135 (MQ=255)                                                                                                           tAACATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACgg                             <  1:775219/135‑1 (MQ=255)                                                                                                            aaCATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTaa                                >  1:165578/1‑131 (MQ=255)                                                                                                            aaCATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTaa                                <  2:165578/131‑1 (MQ=255)                                                                                                             aCATAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaa                      <  1:496887/140‑1 (MQ=255)                                                                                                             aCATAGTTCAATAAGTTCAGTTCCTACAGCCCAGTGGAAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaa                      <  1:401169/140‑1 (MQ=255)                                                                                                               aTAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaatt                    <  2:345987/140‑1 (MQ=255)                                                                                                                tAGTTCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTattaatta                   >  1:639444/1‑140 (MQ=255)                                                                                                                   ttCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTAccc                >  1:2247/1‑140 (MQ=255)                                                                                                                   ttCAATAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTAccc                >  1:346411/1‑140 (MQ=255)                                                                                                                    tCAATAAGTTCAGTTGCCCCAGGCCAGTGGGTGAACTTATTACATAGTACGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCt               <  2:264482/140‑1 (MQ=255)                                                                                                                    tCAATAAGTTCAGTTACTCCAGGCCTGTGGGAGAAGTGATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCt               <  1:338876/140‑1 (MQ=255)                                                                                                                        tAAGTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAag           >  2:515708/1‑140 (MQ=255)                                                                                                                        tAAGATCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATAACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAag           >  2:316133/1‑140 (MQ=255)                                                                                                                           gTTCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAgaga        <  2:721278/140‑1 (MQ=255)                                                                                                                            ttCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGTCTATTAATTACCCTAAAGAGaa       >  1:264482/1‑140 (MQ=255)                                                                                                                             tCAGTTGCTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAGCAGTCAGATAAGGGGTATTAAATACCCTAAAGAGAAg      >  1:247424/1‑140 (MQ=255)                                                                                                                                gttgctCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCa   <  2:14636/140‑1 (MQ=255)                                                                                                                                 ttgctCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCaa  >  2:553613/1‑140 (MQ=255)                                                                                                                                 ttgctCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCaa  <  2:639444/140‑1 (MQ=255)                                                                                                                                 ttgctCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCaa  <  1:641354/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         ATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCATTGTGCTTAACCTTGCCAGTTCAGGCAGATACTTAAACTGGCGTATTTTCTAACATAGTTCAATAAGTTCAGTTGTTCCAGGCCAGTGGGAGAAGTTATTACATAGTGCGTGCAATATCACATTTTTTGCTATGCAATGAATAAAAAGTTATATCACTTTTTCTCATAAAACAGTCAGTTAACGGCTATTAATTACCCTAAAGAGAAGTCAA  >  NC_000913/1907694‑1907961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑