Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,961,461	T→C	V467A (GTA→GCA)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,961,461	0	T	C	97.9%	164.0 / NA	48	V467A (GTA→GCA)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (34/13);  total (34/14)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTGGTTGCCCG  >  NC_000913/1961327‑1961596                                                                                                                                       |                                                                                                                                        cgcgGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGt                                                                                                                                    <  1:132896/140‑1 (MQ=255)             tGGTGCGGTGAAATACGCGGATATCGCCATAAACCGCCCCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTa                                                                                                                        <  1:396406/140‑1 (MQ=255)                tGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAg                                                                                                                     >  1:771289/1‑140 (MQ=255)                tGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAg                                                                                                                     >  1:632128/1‑140 (MQ=255)                    gTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGa                                                                                                                 >  1:652481/1‑140 (MQ=255)                                 aTCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAagag                                                                                                    >  2:135201/1‑140 (MQ=255)                                      tcCAAAAACCGCACCACGGACTACATTTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACt                                                                                               >  2:611365/1‑140 (MQ=255)                                                gCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCt                                                                                     >  2:2505/1‑140 (MQ=255)                                                gCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCt                                                                                     >  2:597747/1‑140 (MQ=255)                                                gCACCACGGACTACATCTTCGACTGGGACAACACGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCt                                                                                     >  2:406656/1‑140 (MQ=255)                                                 caccacGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTc                                                                                    <  2:632128/140‑1 (MQ=255)                                                          cTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTatca                                                                           <  1:629923/140‑1 (MQ=255)                                                            aCATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGGAAAGCAGAAATTGACGGAGAGCAACGGGCTGCAGCT‑CGGTTATCATcc                                                                        >  2:252562/1‑140 (MQ=255)                                                                  tCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGaa                                                                   >  1:647293/1‑140 (MQ=255)                                                                   cGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTatca                                                                           <  2:680556/131‑1 (MQ=255)                                                                   cGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTatca                                                                           >  1:680556/1‑131 (MQ=255)                                                                   cGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGa                                                                    <  1:484671/138‑1 (MQ=255)                                                                   cGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGa                                                                    >  2:484671/1‑138 (MQ=255)                                                                   cGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAg                                                                  >  1:55780/1‑140 (MQ=255)                                                                         ggACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTg                                                            >  2:543085/1‑140 (MQ=255)                                                                           acaacaTGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGaa                                                          <  1:597747/140‑1 (MQ=255)                                                                                            gAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCg                                         >  1:206390/1‑140 (MQ=255)                                                                                             aGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGc                                        <  2:652481/140‑1 (MQ=255)                                                                                                   aTACCGCGCCATACATGCAGTACGCATACACCCGTCCATTGTCCGTGTCCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCctgctg                                  <  2:233576/140‑1 (MQ=255)                                                                                                        gcgcCATACATGCAGTATGCATTCACGCGTGTATTATCCTTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGtt                             <  2:331244/140‑1 (MQ=255)                                                                                                         cgcCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATTATCCGTGAGGATCGTGAAGCGCAACTGGCAGCTCGCCTGATGCAGttt                            >  2:369440/1‑140 (MQ=255)                                                                                                         cgcCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGttt                            >  1:105104/1‑140 (MQ=255)                                                                                                         cgcCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGttt                            >  2:428062/1‑140 (MQ=255)                                                                                                         cgcCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGttt                            >  2:628535/1‑140 (MQ=255)                                                                                                         cgcCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGttt                            >  2:60950/1‑140 (MQ=255)                                                                                                          gcCATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTg                           >  1:581859/1‑140 (MQ=255)                                                                                                           ccATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATTGTGAAGCGCAAATGTCAGCTCACCTGCTGCAGTTTga                          >  1:233576/1‑140 (MQ=255)                                                                                                           ccATACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGCCAGCTCACCTGCTGCAGTTTga                          >  1:301471/1‑140 (MQ=255)                                                                                                             aTACATGCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCGACTGGCGGCTCGCTTGCTGCAGTTTgaag                        >  1:331244/1‑140 (MQ=255)                                                                                                                 aTGCAGTATGCATACCCGCGTGCATTGTCCGTGTTCCTTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCT‑CGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAAcc                   <  1:252562/140‑1 (MQ=255)                                                                                                                   gCAGTATGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAAccc                  >  1:509943/1‑140 (MQ=255)                                                                                                                        aTGCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCAcc             >  1:611404/1‑140 (MQ=255)                                                                                                                          gCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGt           >  1:663693/1‑140 (MQ=255)                                                                                                                          gCATACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGt           <  2:611404/140‑1 (MQ=255)                                                                                                                            aTACACGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTgg         <  2:206390/140‑1 (MQ=255)                                                                                                                              acacGCGTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGTTCGCCTGATGCTGTATGAAGATACCCTCACCGTGGtt       >  1:378699/1‑140 (MQ=255)                                                                                                                                 cgcgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTGGTTGcc    >  1:84414/1‑140 (MQ=255)                                                                                                                                 cgcgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCGCACCGTGGTTGcc    >  2:506706/1‑140 (MQ=255)                                                                                                                                 cgcgTGCATTGTCCGTGTTCC‑TAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGCGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTGGTTGccc   >  1:458559/1‑140 (MQ=255)                                                                                                                                   cgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCTTGCTGCAGTTTGAAGAAACCCTCACCGTGGTTGCCCg  >  1:245547/1‑140 (MQ=255)                                                                                                                                   cgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTgg         <  2:450306/133‑1 (MQ=255)                                                                                                                                   cgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTgg         >  1:450306/1‑133 (MQ=255)                                                                                                                                   cgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTGGTTGCCCg  >  2:85864/1‑140 (MQ=255)                                                                                                                                   cgTGCATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACGGGCAGCTCCCCTACTGCAGTTTGAAGAAACCCTCACCGTGGTTGCCCg  >  2:354202/1‑140 (MQ=255)                                                                                                                                    gtgcATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAAcc                   >  2:519468/1‑122 (MQ=255)                                                                                                                                    gtgcATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAAcc                   <  1:519468/122‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACGGACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATGCAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAACTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTGCTGCAGTTTGAAGAAACCCTCACCGTGGTTGCCCG  >  NC_000913/1961327‑1961596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑