Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,982,680	G→A	G287G (GGC→GGT)	araH ←	arabinose ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,982,680	0	G	A	100.0%	62.2 / NA	25	G287G (GGC→GGT)	araH	arabinose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

CAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCA  >  NC_000913/1982555‑1982813                                                                                                                              |                                                                                                                                      cAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGAt                                                                                                                         >  2:509606/1‑140 (MQ=255)           gTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGccacc                                                                                                               <  1:658120/140‑1 (MQ=255)           gTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGccacc                                                                                                               <  1:762255/140‑1 (MQ=255)             ttCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCcaccac                                                                                                             >  1:818777/1‑140 (MQ=255)                                   cGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCa                                                                                       <  1:528957/140‑1 (MQ=255)                                   cGAAGATCACCGCTGCCAGCAGAATTAAGTCGCGAACTACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATCCCCGCCACCACATATGAGATTTTTCCGATGCCa                                                                                       <  1:348774/140‑1 (MQ=255)                                            ccGCTGCCAGCAGGATTAAGCCGCGAACAACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCaga                                                                              >  2:663206/1‑140 (MQ=255)                                                  ccAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACg                                                                        >  1:128757/1‑140 (MQ=255)                                                    agcagGATTAAGCGGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCGCGGTACCTAAAATTAAGATCCCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGcc                                                                      <  1:468266/140‑1 (MQ=255)                                                                   cgAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACg                                                            <  1:119444/135‑1 (MQ=255)                                                                   cgAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACg                                                            >  2:119444/1‑135 (MQ=255)                                                                   cgAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGc                                                       >  1:117046/1‑140 (MQ=255)                                                                   cgAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGc                                                       >  1:664590/1‑140 (MQ=255)                                                                     aaCCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCgg                                                     <  2:818777/140‑1 (MQ=255)                                                                     aaCCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCgg                                                     <  2:772106/140‑1 (MQ=255)                                                                               tGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAAt                                           <  1:509606/140‑1 (MQ=255)                                                                               tGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAAt                                           <  1:431319/140‑1 (MQ=255)                                                                                gcgcGAAAGGAGAAATATTAACCAGGTCCATGGTGTTTTCCACGGTCCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATc                                          <  1:741838/140‑1 (MQ=255)                                                                                  gcgAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAAt                                           >  2:46267/1‑137 (MQ=255)                                                                                  gcgAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAAt                                           <  1:46267/137‑1 (MQ=255)                                                                                    gAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACTGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCt                                      <  2:323495/140‑1 (MQ=255)                                                                                                    aGCAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATATCAATCAGCTGATAACCAATCGACGt                      >  1:337338/1‑140 (MQ=255)                                                                                                      cAGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCa                    >  2:673760/1‑140 (MQ=255)                                                                                                       aGGTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCAt                   <  1:207405/140‑1 (MQ=255)                                                                                                         gTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTg                 >  1:720584/1‑140 (MQ=255)                                                                                                         gTTCATGGCGTTTTCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTg                 >  2:7010/1‑140 (MQ=255)                                                                                                            cATGGCGTTTTCCAGGGTCCTTAAAATTAAGATCCCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCt              <  2:720584/140‑1 (MQ=255)                                                                                                                   ttttCCACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACt       >  2:590553/1‑140 (MQ=255)                                                                                                                        cACGGTACCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCa  <  2:128757/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                      CAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTTCAGAGAAACGCCACCTAAAACGCAGGCGGAGATAACAATCAGCTCATAACCAATCGACGTCATTGGCTGCCCACTGGTCA  >  NC_000913/1982555‑1982813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑