Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,009,080	T→C	S268S (AGT→AGC)	yedE →	putative selenium transporter YedE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,009,080	0	T	C	100.0%	19.4 / NA	7	S268S (AGT→AGC)	yedE	putative selenium transporter YedE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/6);  total (1/6)

GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAA  >  NC_000913/2008945‑2009203                                                                                                                                        |                                                                                                                            ggCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCa                                                                                                                         <  1:9469/140‑1 (MQ=255)    aaTGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGGGCGCCATCg                                                                                                                      <  2:202394/140‑1 (MQ=255)      tGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCATCggg                                                                                                                    <  1:795534/140‑1 (MQ=255)         tGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCATCGGGATc                                                                                                                 <  1:819985/140‑1 (MQ=255)           tttGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCATCGGGATCtt                                                                                                               <  1:799677/140‑1 (MQ=255)            ttGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGGGGGCCACCGGGGTCttt                                                                                                              >  2:795534/1‑139 (MQ=255)                                     tGAACGTGCGCAAATCTGTTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGGGGGCCAACCGGGGGTTCCGTTACGTAAAGTTAGGGGTTTaaa                                                                                     >  1:795113/1‑139 (MQ=255)                                          gTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCATCGAGGTCTTCGGTTACGTAAAGTTGGGCGTTGAACCCaaa                                                                                >  1:228677/1‑140 (MQ=255)                                          gTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGAGCCAACTGGGCCTTCAGGTACGTACAATTAGGGGTTGTACCCcaa                                                                                >  1:707448/1‑140 (MQ=255)                                           tGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGGGCCACCGGGGTCTTCAGTTACGTAAAGTTAGGGGTTGTACCCaaaa                                                                               >  1:476608/1‑140 (MQ=255)                                           tGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCATCGGGGTCTTCAGTTACGTAAAGTTAGGCGTTGAACCCaaaa                                                                               >  1:419520/1‑140 (MQ=255)                                             cgcAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGGGAGCGCCAACCGGAGCTTCCATTTCGTACAGCTAGGCGGTGAACCCcaaaat                                                                             >  2:434782/1‑138 (MQ=255)                                                                aGCGTTCCGCGATATGTGGATCGCCGGACGTTCCCTTATGGCGAAAGCTATCATTATCGGTATGGCGGTGAGCGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAAcg                                                          <  2:228677/140‑1 (MQ=255)                                                                    ttCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGGGCCATCGGGGTCTTCAGTTACGGACAGTTAGGGGTATAAACCAAAAACATGTGGGGGGGGGCAAACGCggg                                                      >  2:317851/1‑139 (MQ=255)                                                                      ccGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGCGCCCTCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAAACCAAAATCATGTGGGCGGGGCCCAACGCGGGaa                                                    >  2:713723/1‑140 (MQ=255)                                                                                                                  tCATTATCGGTATGGCGGTGAGCGCCCTCGGGGGCTTCAGTTACGTACAGTTTGGGGTTGAAACCAAAAACATTGGGGCGGGGGCAAACGCGGGGATTGGTGGGGTTCTGTTTTGTTTTTGCATCCTGCTGGCTggcggg        >  2:606748/1‑139 (MQ=255)                                                                                                                        tCGGTATGGTGGTGAGCGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGaa  >  2:123897/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                            GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACTGTTTGGTTTTGGCATCGTGCTGGCTGGCGGCTGCGAA  >  NC_000913/2008945‑2009203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑