Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,017,228 T→C G225G (GGT→GGC)  fliI → flagellum‑specific ATP synthase FliI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,017,2280TC100.0% 129.4 / NA 39G225G (GGT→GGC) fliIflagellum‑specific ATP synthase FliI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/17);  total (22/17)

TTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGTGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTA  >  NC_000913/2017094‑2017364
                                                                                                                                      |                                                                                                                                        
tttGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGa                                                                                                                                     >  1:71498/1‑140 (MQ=255)
    ccGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAggg                                                                                                                                 >  2:471293/1‑140 (MQ=255)
         tCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGc                                                                                                                            <  1:509161/140‑1 (MQ=255)
           cGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCAc                                                                                                                          >  2:446671/1‑140 (MQ=255)
                ttGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCa                                                                                                                     <  1:446671/140‑1 (MQ=255)
                     aaaaGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAgtggt                                                                                                                <  2:436812/140‑1 (MQ=255)
                             gctgctGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCg                                                                                                        >  1:449455/1‑140 (MQ=255)
                                   ggggATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCcgg                                                                                                  >  1:814144/1‑140 (MQ=255)
                                    gggATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCcggc                                                                                                 >  1:537650/1‑140 (MQ=255)
                                                   tACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCt                                                                                  >  2:64673/1‑140 (MQ=255)
                                                   tACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGCGCGTGCACCCTCCGTGGTGATTGCCGCTCCTGCGGATGTTTCTCCGCt                                                                                  >  2:313826/1‑140 (MQ=255)
                                                       cccGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGAGGTGATTCCCGATCGGGCGGAGGTTTCTCCGCTCCTg                                                                              >  1:394787/1‑140 (MQ=255)
                                                       cccGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGGGCACGCTCGGTGTTGATTGCCGCTCTGGCGAATGTTTCTCCGCTCCTg                                                                              >  2:266098/1‑140 (MQ=255)
                                                        ccGCGCCGCTGTCATTTGCGTGTGTTTGATTTGGGAACGCGGGCGCGATGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGc                                                                             <  2:394787/140‑1 (MQ=255)
                                                        ccGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGc                                                                             >  2:698162/1‑140 (MQ=255)
                                                           cgcCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCAAGCTCAGTGATGATTACCTCTCCGGCGGATGTTTCTCCGCACCTGCGaa                                                                          >  2:376580/1‑140 (MQ=255)
                                                              agATGTCATTGTCGTAGGTTTGTTTAGTGTACGTGGGTGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGc                                                                       <  1:233100/139‑1 (MQ=255)
                                                               gATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCa                                                                      <  1:138877/140‑1 (MQ=255)
                                                                   tCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGt                                                                  <  1:471293/140‑1 (MQ=255)
                                                                     aTTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTgc                                                                <  1:64673/140‑1 (MQ=255)
                                                                           gTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGACGCTCCGGGGGATGTTCCTCCGCTCGTGCGAATGCAGGGTgccgccct                                                          >  2:233100/1‑138 (MQ=255)
                                                                             gggTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGCATGTTTCGCCGCTCCTGCGAAGGCAGGGTGCCGCCTATg                                                        >  2:256464/1‑140 (MQ=255)
                                                                                      tGGTGAACGTGGGCGCGAAGTAGAATATTGTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCa                                               <  1:256464/140‑1 (MQ=255)
                                                                                        gTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCAtt                                             >  1:425056/1‑140 (MQ=255)
                                                                                         tGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTACTGCGAATGCAAGGCGCCGCCGATGCCACGCGCATTg                                            >  1:280660/1‑140 (MQ=255)
                                                                                          gAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCGTGCGAATGCAGAGTGCCGCCTGTGCCACGCGCATTGc                                           >  1:216703/1‑140 (MQ=255)
                                                                                                 ggCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGCGCCGCCTGTGCCACGCGCATTCCCGAAGAt                                    >  2:377886/1‑140 (MQ=255)
                                                                                                          tAAAAGATTTTATTGAGAACATGCTCGGCTCCGAAGGCCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGAt                           <  1:266098/140‑1 (MQ=255)
                                                                                                            aaaGATTTTATTGAGAACATCCGCGGCGCCGGAGGGCGTTCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCg                         <  2:280660/140‑1 (MQ=255)
                                                                                                              aGATTTTATTGAGAACATCCTCCGCACCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGTCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTg                       <  1:313826/140‑1 (MQ=255)
                                                                                                              aGATTTTATTGAGAACACCCTCTGCGCCGAAAGTCCTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTg                       <  2:367700/140‑1 (MQ=255)
                                                                                                                  tttATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCa                   >  2:558019/1‑140 (MQ=255)
                                                                                                                   ttATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAg                  >  1:188728/1‑140 (MQ=255)
                                                                                                                    tATTGAGAACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATACCACGCGCATTGCCGAGGATTGTCGCGATCGTGGTCAGc                 >  2:260995/1‑140 (MQ=255)
                                                                                                                        gagaACATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCAtgt             <  2:762328/140‑1 (MQ=255)
                                                                                                                           aaCATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCCTTGCCGACGATTTTTGCGATCCTGGTCAGCAtgtgct          >  1:367700/1‑138 (MQ=255)
                                                                                                                            aCATCCTCGGCGCCGTAGGGCGAGCTCGCACAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTg         <  1:376580/140‑1 (MQ=255)
                                                                                                                            aCATCCTCGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTg         <  2:537650/140‑1 (MQ=255)
                                                                                                                                  tCGGCGCCGAAGGGCATGCACGCTCCGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGAtt   <  1:260995/140‑1 (MQ=255)
                                                                                                                                   cGGCGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTa  <  2:188728/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TTTGCCGGGTCCGGCGTTGGTAAAAGTGTGCTGCTGGGGATGATGGCACGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGTGGGCGCGAAGTAAAAGATTTTATTGAGAACATCCTCGGTGCCGAAGGGCGTGCACGCTCAGTGGTGATTGCCGCTCCGGCGGATGTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTA  >  NC_000913/2017094‑2017364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑