Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,030,935	A→C	R461R (CGT→CGG)	dcm ←	DNA‑cytosine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,030,935	0	A	C	100.0%	93.5 / NA	28	R461R (CGT→CGG)	dcm	DNA‑cytosine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/11);  total (17/11)

GCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCC  >  NC_000913/2030806‑2031070                                                                                                                                  |                                                                                                                                        gCCAGGCCCTGCCCGGTTAACAGACTGGCGAGCCGCTTCTCTATCGCCGTATCACGCGTGGCCATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGCCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCa                                                                                                                               >  2:177650/1‑140 (MQ=255)     ggCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGc                                                                                                                           <  1:579526/140‑1 (MQ=255)          tGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGtt                                                                                                                      >  2:104312/1‑140 (MQ=255)                gTTAACAGACTGGCTAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGAttt                                                                                                                <  1:430008/140‑1 (MQ=255)                gTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGCGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGAttt                                                                                                                >  2:516046/1‑140 (MQ=255)                      aGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCt                                                                                                          >  2:410392/1‑140 (MQ=255)                      aGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCt                                                                                                          <  2:444720/140‑1 (MQ=255)                          gggCGAGGCGCTTCTCTGCCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAg                                                                                                      <  2:609111/139‑1 (MQ=255)                           ggCGAGGCGCTTCTCTGCCGCCTTATCCCGCTGAGTAATCGCGCGCATGTTTTTGCTGCGAGTGCCCTTGTCGTGAACGTCGGCCTTGTTGTGCGTTTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGc                                                                                                     <  2:368093/140‑1 (MQ=255)                           ggCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGc                                                                                                     >  2:77019/1‑140 (MQ=255)                           ggCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGc                                                                                                     >  2:157320/1‑140 (MQ=255)                               aGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGTAACGCCAGCGCGTGTTTGATTATTGGCTCAAGCAGtt                                                                                                 >  1:248223/1‑140 (MQ=255)                                               ccGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCaaa                                                                                 >  1:801595/1‑140 (MQ=255)                                               ccGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTGTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCaaa                                                                                 >  1:318227/1‑140 (MQ=255)                                                 gTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCCCCGCCTGTTTGATTTTTGGCTCAAGGAGTTGTGCCACCGCGGCAAAGa                                                                               >  2:312564/1‑140 (MQ=255)                                                     cACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCgg                                                                           <  1:104312/140‑1 (MQ=255)                                                       cgcgTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCa                                                                         <  1:429722/140‑1 (MQ=255)                                                            ggCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGacc                                                                    >  1:22291/1‑140 (MQ=255)                                                               aaTCGCGCGCATATTTTTGCTGTGAGTGGCCGTATTGTGCACGTCGGCAATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGaccacc                                                                 <  2:318227/140‑1 (MQ=255)                                                                       gcATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTAcc                                                         <  2:603713/140‑1 (MQ=255)                                                                                tgctgcGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCg                                                <  2:22291/140‑1 (MQ=255)                                                                                   tgcGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGGTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTa                                                           >  2:453135/1‑126 (MQ=255)                                                                                   tgcGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGGTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTa                                                           <  1:453135/126‑1 (MQ=255)                                                                                        gTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCt                                        <  1:410392/140‑1 (MQ=255)                                                                                               tATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTc                                 >  2:586348/1‑140 (MQ=255)                                                                                                      aaCGTCGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAAcc                          >  1:647721/1‑140 (MQ=255)                                                                                                           cGGCCATGTTGTGCCTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAAt                     >  2:567994/1‑140 (MQ=255)                                                                                                                        ccTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCt        >  2:46145/1‑140 (MQ=255)                                                                                                                        ccTCTTGCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCt        >  2:777519/1‑140 (MQ=255)                                                                                                                              gCTGCCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTccc  >  2:538737/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        GCCAGGCCCTGCCCGGTTAACAGACTGGCGAGGCGCTTCTCTATCGCCGTATCACGCGTGGCAATCGCGCGCATATTTTTGCTGCGAGTGGCCTTATCGTGAACGTCGGCCATGTTGTGCCTCTTGCTGACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCC  >  NC_000913/2030806‑2031070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑