Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,060,312	A→G	I201I (ATT→ATC)	cbl ←	DNA‑binding transcriptional activator Cbl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,060,312	0	A	G	100.0%	186.9 / NA	54	I201I (ATT→ATC)	cbl	DNA‑binding transcriptional activator Cbl
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

TGCTCGGCAACTAATCCGATCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTG  >  NC_000913/2060178‑2060446                                                                                                                                       |                                                                                                                                       tGCTCGGCAACTAATCCGATCCTAACCCCCAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATccc                                                                                                                                   <  1:300283/140‑1 (MQ=255) tGCTCGGCAACTAATACGATCCCAAGCCCCAGAGCAATATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATccc                                                                                                                                   <  1:335096/140‑1 (MQ=255)   cTCGGCAACTAATCCGATCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAAGCTTTGCGGGCAAATGCATCATCAATACGTGGGCGCCCCGTGATCCCCt                                                                                                                                 >  2:335096/1‑140 (MQ=255)    tCGGCAACTAATCCGATCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTg                                                                                                                                <  2:62915/140‑1 (MQ=255)     cGGCAACTAATCCGATCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGt                                                                                                                               >  1:823520/1‑140 (MQ=255)        cAACTAATCCGATCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCgg                                                                                                                            >  1:44893/1‑140 (MQ=255)                    tCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAAc                                                                                                                >  1:508158/1‑140 (MQ=255)                       caagcccaagAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGc                                                                                                             <  1:501971/140‑1 (MQ=255)                        aagcccaagAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGcc                                                                                                            >  1:537686/1‑140 (MQ=255)                              aagAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCg                                                                                                      >  2:692894/1‑140 (MQ=255)                                gagCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCt                                                                                                    <  1:128890/140‑1 (MQ=255)                                         aGGTTTTAATGACATCAGAATCTTGAGCACTTAATACTATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTcc                                                                                           <  1:349229/140‑1 (MQ=255)                                           gTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTTGGTAAGTGATTAACAGCCACTTCGCTATTGATTCCAg                                                                                         >  1:266680/1‑140 (MQ=255)                                                aaTGACATCAGAATCCTGCGCACTTAATACAATTTCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCa                                                                                    <  2:387809/140‑1 (MQ=255)                                                aaTGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCa                                                                                    <  2:513614/140‑1 (MQ=255)                                                aaTGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCa                                                                                    >  1:194137/1‑140 (MQ=255)                                                aaTGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCATGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCa                                                                                    >  1:577859/1‑140 (MQ=255)                                                  tGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAAt                                                                                  >  2:200560/1‑140 (MQ=255)                                                      aTCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTg                                                                              >  1:749055/1‑140 (MQ=255)                                                      aTCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTg                                                                              >  2:818336/1‑140 (MQ=255)                                                            aTCCTGCGCACTTAACACAATATCTGCCATCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAAttt                                                                        <  1:295827/140‑1 (MQ=255)                                                                tGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGt                                                                    <  1:599264/140‑1 (MQ=255)                                                                agacagcTTAATACAATATCTGTCAGCAAACCTTTCCGGTCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGt                                                                    <  2:289648/134‑1 (MQ=255)                                                                  cgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTAGCTATTGGTTCGAGGGTCAATAGTGAAATTTGCGTCa                                                                  >  1:217072/1‑140 (MQ=255)                                                                     aCTTAATACAATATCTGCCAGCCAACCTTTGCGGGCAAACGCGTCATCTATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAgg                                                               <  2:217072/140‑1 (MQ=255)                                                                       ttAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGga                                                             >  1:774831/1‑140 (MQ=255)                                                                       ttAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGga                                                             <  1:105073/140‑1 (MQ=255)                                                                         aaTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCa                                                                  <  1:15539/133‑1 (MQ=255)                                                                         aaTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCa                                                                  >  2:15539/1‑133 (MQ=255)                                                                         aaTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGgatg                                                           >  1:823329/1‑140 (MQ=255)                                                                          aTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGgatga                                                          <  2:194137/140‑1 (MQ=255)                                                                          aTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGgatga                                                          <  1:692894/140‑1 (MQ=255)                                                                          aTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCATGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGgatga                                                          <  2:577859/140‑1 (MQ=255)                                                                            aCAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATc                                                        >  1:823212/1‑140 (MQ=255)                                                                                 atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTTTTGATTCCGGCGGCGATGGTGAAATGTGCGGGAAGGGATGATCGtgtg                                                   >  2:403605/1‑140 (MQ=255)                                                                                 atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTCTTGATTCCAGCGACAAATGTGAAATATGCGGGAAGGGATGATCGtgtg                                                   >  2:382526/1‑140 (MQ=255)                                                                                 atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGtgtg                                                   >  2:28193/1‑140 (MQ=255)                                                                                 atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGtgtg                                                   >  2:611592/1‑140 (MQ=255)                                                                                 atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGAGTCAATGGTGAAATATGCGTCAAGGGATGATctgtct                                                   >  1:289648/1‑135 (MQ=255)                                                                                   cTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGga                                                             >  1:429252/1‑128 (MQ=255)                                                                                   cTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGga                                                             <  2:429252/128‑1 (MQ=255)                                                                                    tGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGgatg                                                           >  2:647643/1‑129 (MQ=255)                                                                                    tGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGgatg                                                           <  1:647643/129‑1 (MQ=255)                                                                                    tGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGaa                                                <  1:28193/140‑1 (MQ=255)                                                                                     gCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAAc                                               >  1:20927/1‑140 (MQ=255)                                                                                          cAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATAGTGAAATATGCGTCAAAGGATCATCGTGTGGAACAAGCa                                          >  2:264130/1‑140 (MQ=255)                                                                                          cAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAG‑GATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCaa                                         >  1:651401/1‑140 (MQ=255)                                                                                                    cGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTg                                <  2:563792/140‑1 (MQ=255)                                                                                                           aTGCGTCATCAATACGTGAGCGCCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGa                         <  2:823329/140‑1 (MQ=255)                                                                                                                              gcgcCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGcgacga      >  2:614484/1‑140 (MQ=255)                                                                                                                               cgcCCCGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAg     <  2:20927/140‑1 (MQ=255)                                                                                                                                 ccccGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCTAACAATGGAGCCAACGCAACCAAGGGAAGGGGACGAGCt   >  2:380206/1‑140 (MQ=255)                                                                                                                                  cccGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCTATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTCTTGTGCCAACGAAACCACGGGAAGGCGACGAGCTg  >  2:367646/1‑140 (MQ=255)                                                                                                                                  cccGTGATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTg  <  1:200560/140‑1 (MQ=255)                                                                                                                                  cccGTGATCCCCTGTAGGTAAGCGACTAAGGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAATGAAACCACGGGAAGGCGACGAGCTg  <  1:382526/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGCTCGGCAACTAATCCGATCCCAAGCCCAAGAGCAACATAGGTTTTAATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTG  >  NC_000913/2060178‑2060446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑