Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,102	T→C	V301A (GTG→GCG)	yagF →	CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,102	0	T	C	100.0%	62.7 / NA	19	V301A (GTG→GCG)	yagF	CP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/11);  total (8/11)

CGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTG  >  NC_000913/283970‑284238                                                                                                                                     |                                                                                                                                         cgcTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCACCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCGCTCCGATAAAGTCAACGAAAACGCGATGGCGATCCAc                                                                                                                                   >  1:305686/1‑140 (MQ=255)  gcTGGCGCCGCCCGGCAAGGCGCTGGGGTTGGAGATCACCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCAcg                                                                                                                                  <  1:367701/140‑1 (MQ=255)     ggCGCGGTCCGGGCGGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACgcgg                                                                                                                               <  1:813979/140‑1 (MQ=255)       cgcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGGAACCGCGATAGCGATCCAcctgtcg                                                                                                                             >  1:355340/1‑134 (MQ=255)       cgcCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACgcggcg                                                                                                                             >  1:663401/1‑140 (MQ=255)                          ggggcgggcgACCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAAtt                                                                                                          <  2:46845/138‑1 (MQ=255)                                   gaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACtgctgc                                                                                                 >  1:530476/1‑140 (MQ=255)                                   gaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACtgctgc                                                                                                 >  2:150955/1‑140 (MQ=255)                                   gaTCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACtgctgc                                                                                                 >  2:158908/1‑140 (MQ=255)                                       gcccgccAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACAt                                                                                             >  2:580650/1‑140 (MQ=255)                                                          cgGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCAcgcg                                                                          <  2:663401/140‑1 (MQ=255)                                                                    gCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCa                                                                <  2:530476/140‑1 (MQ=255)                                                                    gCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCa                                                                <  2:435158/140‑1 (MQ=255)                                                                             ccGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCgg                                                       <  1:158908/140‑1 (MQ=255)                                                                                cgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACg                                                    <  1:150955/140‑1 (MQ=255)                                                                                                   atCCTCTCCGATAAAGCCATCGTAAGCGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCAt                                 <  1:373606/140‑1 (MQ=255)                                                                                                        ctcCGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAcc                            >  2:482419/1‑140 (MQ=255)                                                                                                          ccGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGa                                        <  1:760259/126‑1 (MQ=255)                                                                                                          ccGATAAAGCCATCGAAAACGCGATGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGa                                        >  2:760259/1‑126 (MQ=255)                                                                                                                                  tGGCGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGtg  <  1:695657/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         CGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTG  >  NC_000913/283970‑284238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑