Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,101,186	G→A	T28I (ACC→ATC)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,101,186	0	G	A	100.0%	251.3 / NA	75	T28I (ACC→ATC)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/40);  total (35/40)

TAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTCGCGGCCT  >  NC_000913/2101053‑2101315                                                                                                                                      |                                                                                                                                  tAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAAc                                                                                                                             >  1:407808/1‑140 (MQ=255)   aCAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCa                                                                                                                           <  2:820359/140‑1 (MQ=255)   aCAGGATGCGACGAGGCGTTTCCAGAGATTCCACATACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCa                                                                                                                           <  2:329504/140‑1 (MQ=255)     aGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACg                                                                                                                         <  2:407808/140‑1 (MQ=255)        aTGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTTCGTCTTCTGACGTGAAAGGTTGAAAATAGAAACGATATAAcctgtctc                                                                                                                      >  2:276858/1‑134 (MQ=255)        aTGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCt                                                                                                                      >  2:582952/1‑140 (MQ=255)        aTGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCt                                                                                                                      >  1:129386/1‑140 (MQ=255)        aTGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGAGAAGGGTTGAAACTAGACACAATATAACCACGGCt                                                                                                                      >  2:305540/1‑140 (MQ=255)         tGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCtt                                                                                                                     >  2:796927/1‑140 (MQ=255)           cgacgaGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTc                                                                                                                   >  2:70408/1‑140 (MQ=255)            gacgaGGCGTTTCCACAGATTCGACGAACACTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCg                                                                                                                  <  1:380788/140‑1 (MQ=255)              cgaGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGCACGTTTGGAAATAGAGCCGATATAACCACGCCTTTCGAt                                                                                                                >  2:403757/1‑140 (MQ=255)                       tCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAgcgc                                                                                                       <  1:70408/140‑1 (MQ=255)                            agaTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGt                                                                                                  >  2:761942/1‑140 (MQ=255)                              aTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCg                                                                                                                  <  2:797435/122‑1 (MQ=255)                              aTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCg                                                                                                                  >  1:797435/1‑122 (MQ=255)                              aTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTg                                                                                                <  1:801494/140‑1 (MQ=255)                              aTTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTg                                                                                                <  1:747948/140‑1 (MQ=255)                                tCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCg                                                                                              >  1:424105/1‑140 (MQ=255)                                tCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCg                                                                                              >  2:302926/1‑140 (MQ=255)                                      aaCTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCAt                                                                                        <  1:422111/140‑1 (MQ=255)                                      aaCTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCAt                                                                                        <  2:424105/140‑1 (MQ=255)                                       aCTCTTTCACCGTATCGTAAGGAACTAGTATCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATc                                                                                       <  1:296472/140‑1 (MQ=255)                                       aCTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCAt                                                                                        >  2:573638/1‑139 (MQ=255)                                       aCTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCAt                                                                                        <  1:573638/139‑1 (MQ=255)                                                   tataGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCg                                                                           >  1:580844/1‑140 (MQ=255)                                                   tataGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCCCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCg                                                                           >  2:660107/1‑140 (MQ=255)                                                   tataGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGACTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCg                                                                           >  1:452710/1‑140 (MQ=255)                                                      aGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACt                                                                        <  2:64578/140‑1 (MQ=255)                                                         aaGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACg                                                                     >  2:746237/1‑140 (MQ=255)                                                         aaGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACg                                                                     <  1:761942/140‑1 (MQ=255)                                                          aGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGc                                                                    <  1:576588/140‑1 (MQ=255)                                                           ggAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGcc                                                                   >  1:9261/1‑140 (MQ=255)                                                             aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCCAGGTTGCTTCCAATCACTGCCTTACCGACTACGCCGa                                                                 >  1:304790/1‑140 (MQ=255)                                                             aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGa                                                                 >  1:623010/1‑140 (MQ=255)                                                             aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGa                                                                 >  2:669603/1‑140 (MQ=255)                                                             aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGa                                                                 >  1:141170/1‑140 (MQ=255)                                                             aaCCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTCCGTCCCATGACTGACATACCGACTACGCCGa                                                                 >  2:249002/1‑140 (MQ=255)                                                               ccAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATc                                                               >  1:643917/1‑140 (MQ=255)                                                               ccAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCAATGCCATACCGACTACGCCGATc                                                               >  1:363005/1‑140 (MQ=255)                                                                cAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGATTGCGTCGCATGACGGCCATAGCGACTACGCCGATCt                                                              >  1:211424/1‑140 (MQ=255)                                                                  gTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGTGTCCCATCACTGCCATACCGACTACGCCGATCTGt                                                            >  2:345029/1‑140 (MQ=255)                                                                  gTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCCTCACTGCCATACCAACTACGCCGATCTGt                                                            >  1:311999/1‑140 (MQ=255)                                                                      cTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCt                                                        <  1:137214/140‑1 (MQ=255)                                                                       ttGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCTTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCtt                                                       <  1:669603/140‑1 (MQ=255)                                                                       ttGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCtt                                                       <  2:141170/140‑1 (MQ=255)                                                                         gCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTgg                                                     <  2:9261/140‑1 (MQ=255)                                                                         gCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTgg                                                     <  1:467194/140‑1 (MQ=255)                                                                         gCCTGGATTTTCGGCAATCACTCCTTCCGTCCTCTGACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTgg                                                     <  2:311999/140‑1 (MQ=255)                                                                          ccTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCTTCACTGCTATACCGGCTACGCAGATCTGTTGCTTGGa                                                    >  1:301377/1‑140 (MQ=255)                                                                          ccTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGa                                                    <  1:493543/140‑1 (MQ=255)                                                                          ccTGGATTTTCGGCAATCACTTCTTCCGTCTCCTCCCGGGAACGGTTGTAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGa                                                    <  2:211424/140‑1 (MQ=255)                                                                            tGGATTTTCGGCAATGACTTCTTCCATCTACTCACGGCAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACa                                                  <  1:347702/140‑1 (MQ=255)                                                                            tGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACa                                                  <  2:452710/140‑1 (MQ=255)                                                                            tGGATTTTCGGCAATCACTTCTTCCGTCTCCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACa                                                  <  1:403757/140‑1 (MQ=255)                                                                            tGGATTTTCGGCAATAACTTCTTCCTTCTACTCACGGTAACGGTTGAAAATAGAGACAATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACa                                                  <  1:305540/140‑1 (MQ=255)                                                                            tGGATTTTCGGCAATAACTTCTCCCGTCTTCTCACGGTAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACa                                                  <  1:296608/140‑1 (MQ=255)                                                                              gATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACAtt                                                <  1:302926/140‑1 (MQ=255)                                                                                ttttCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTAc                                              <  1:796927/140‑1 (MQ=255)                                                                                      gCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTc                                        <  2:84593/140‑1 (MQ=255)                                                                                        aaTCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCt                                      >  1:84836/1‑140 (MQ=255)                                                                                          tCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGt                                    <  2:363005/140‑1 (MQ=255)                                                                                                    cGTCTTCTCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGAt                          <  1:821578/140‑1 (MQ=255)                                                                                                      tcttctCACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCa                        >  2:604403/1‑140 (MQ=255)                                                                                                          ctcACGGGAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACcgc                    >  2:744309/1‑140 (MQ=255)                                                                                                                ggAACGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGaa              <  1:452969/140‑1 (MQ=255)                                                                                                                  aaCGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATg            >  2:7308/1‑140 (MQ=255)                                                                                                                    cGGTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCt          >  2:472091/1‑140 (MQ=255)                                                                                                                     ggTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTc         <  1:650147/140‑1 (MQ=255)                                                                                                                     ggTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGAGTGATCACCGCGCTGAATGCTc         <  2:623010/140‑1 (MQ=255)                                                                                                                      gTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTcg        <  1:582952/140‑1 (MQ=255)                                                                                                                      gTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTcg        <  2:626193/140‑1 (MQ=255)                                                                                                                      gTTGAAAATAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTcg        <  1:660107/140‑1 (MQ=255)                                                                                                                      gTTGAAAATAGAGACGACATAAACACGGCTTGCGAGGTTGAGCGCAAGATTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTcg        <  2:304790/140‑1 (MQ=255)                                                                                                                            aaTAGAGACGATATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTCGCGGCCt  >  2:166242/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                  TAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGCTTTCGATGTTGAGCGCAAGGTTGCGTCCCATCACTGCCATACCGACTACGCCGATCTGTTGCTTGGACATTACATACTCCTGTCAGGTGTGATCACCGCGCTGAATGCTCGCGGCCT  >  NC_000913/2101053‑2101315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑