Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,136,843	A→G	V134A (GTC→GCC)	wza ←	outer membrane polysaccharide export protein Wza

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,136,843	0	A	G	100.0%	90.3 / NA	27	V134A (GTC→GCC)	wza	outer membrane polysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

TAGGCCTTTTGTGAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCC  >  NC_000913/2136723‑2136977                                                                                                                         |                                                                                                                                       taGGCCTTTTGTGAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTa                                                                                                                     >  2:121024/1‑140 (MQ=255)          tgtgAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGt                                                                                                            <  1:482650/140‑1 (MQ=255)                                       cACCTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGAGCCATCCGGCTGTGCCCAGTTGCCGgtgt                                                                               >  1:235649/1‑140 (MQ=255)                                         ccTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATTTACGGGTAGAACATAGTGCCGTCAGTCTGTTCCCAGTTGCCGGTGTCg                                                                             >  2:262300/1‑140 (MQ=255)                                         ccTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCg                                                                             >  1:576705/1‑140 (MQ=255)                                               ggTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAg                                                                       <  1:575053/140‑1 (MQ=255)                                                  cAGCGATGTACGTCGCTAAGCGTCCGGTAATATCACTCCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTg                                                                    <  1:318635/140‑1 (MQ=255)                                                  cAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTg                                                                    <  1:599478/140‑1 (MQ=255)                                                   aGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGc                                                                   >  1:536837/1‑140 (MQ=255)                                                               cGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCgg                                                       >  1:760449/1‑140 (MQ=255)                                                               cGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCgg                                                       <  1:121024/140‑1 (MQ=255)                                                                    aGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGc                                                  >  2:548145/1‑140 (MQ=255)                                                                       gcCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCgtg                                               >  1:178266/1‑140 (MQ=255)                                                                         ccGGTAATATCACTGCGGATTTCAGTGAGCGTTTTCCAGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCgtggt                                             <  1:262300/140‑1 (MQ=255)                                                                               atatCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTc                                       <  2:649105/140‑1 (MQ=255)                                                                                   cACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCggg                                   <  1:548145/140‑1 (MQ=255)                                                                                    aCTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGt                                  <  2:536837/140‑1 (MQ=255)                                                                                       gCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGt                                  >  2:24439/1‑137 (MQ=255)                                                                                       gCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGt                                  <  1:24439/137‑1 (MQ=255)                                                                                       gCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGAt                               >  1:597921/1‑140 (MQ=255)                                                                                       gCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGAt                               >  1:96679/1‑140 (MQ=255)                                                                                         ggATTTCAGCGAGCGTTATCCCCACTACGTGCGCCCTGCTGATATACGTGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATcc                             <  2:235649/140‑1 (MQ=255)                                                                                                 gcgagcgTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGt                     <  1:76430/140‑1 (MQ=255)                                                                                                 gcgagcgTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGt                     <  2:576705/140‑1 (MQ=255)                                                                                                  cgagcgTTTTCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGCCCGGCGGCGGTGGTGAGTTCCGGGTGATCCGAGACGGTg                    >  2:379890/1‑140 (MQ=255)                                                                                                           tCCCGACTACGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGa           <  2:96679/140‑1 (MQ=255)                                                                                                                    cGTGGGCCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTccccc  >  1:680679/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                       TAGGCCTTTTGTGAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATGTACGTCGCTAAGCGCCCGGTAATATCACTGCGGATTTCAGCGAGCGTTTTCCCGACTACGTGGACCTTGCCGATATACGGGTAAAACATAGTGCCGTCAGGCTGTACCCAGTTGCCGGTGTCGCTGGAGCTGCGGTACTGACCGGCTGGCGTGGTGAGTTCCGGGTGATCCCAGACGGTGACATTAAGAACGTCCCCC  >  NC_000913/2136723‑2136977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑