Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,791	T→C	L27P (CTG→CCG)	yegS →	lipid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,791	0	T	C	100.0%	292.2 / NA	82	L27P (CTG→CCG)	yegS	lipid kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/37);  total (45/37)

ACCTTTTGTTTTCTGCTCTACACTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCTGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGC  >  NC_000913/2168655‑2168915                                                                                                                                         |                                                                                                                             aCCTTTTGTTTTCTGCTCTACACTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGtt                                                                                                                           >  1:181673/1‑140 (MQ=255)             cTGCTCTACACTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGaggaagg                                                                                                               <  2:535982/140‑1 (MQ=255)                ctctACACTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAAt                                                                                                            >  1:542932/1‑140 (MQ=255)                ctctACACTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCCTGCGCGATGCAGTTATGCCGTGGCGTGAGGAAGGAAt                                                                                                            >  1:330878/1‑140 (MQ=255)                     cacTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGagga                                                                                                                  >  2:153540/1‑129 (MQ=255)                     cacTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGagga                                                                                                                  <  1:153540/129‑1 (MQ=255)                     cacTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGa                                                                                                       >  2:594967/1‑140 (MQ=255)                          tCTACAGACCAATTATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGa                                                                                                          >  2:16900/1‑132 (MQ=255)                          tCTACAGACCAATTATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGa                                                                                                          <  1:16900/132‑1 (MQ=255)                          tCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCAt                                                                                                  >  1:110584/1‑140 (MQ=255)                          tCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCAt                                                                                                  >  1:726675/1‑140 (MQ=255)                          tCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCAt                                                                                                  >  2:486024/1‑140 (MQ=255)                          tCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCAt                                                                                                  >  2:597536/1‑140 (MQ=255)                          tCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGACGTAGCGTGAGGACGGAATGACGATCCAt                                                                                                  >  1:251394/1‑140 (MQ=255)                             aCAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCAtgtg                                                                                               >  2:82538/1‑140 (MQ=255)                               aGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCg                                                                                             >  2:729376/1‑140 (MQ=255)                               aGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCg                                                                                             >  1:498909/1‑140 (MQ=255)                               aGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTACGTGAGGAAGGAAAGACGATCCATGTGCg                                                                                             >  1:264283/1‑140 (MQ=255)                               aGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAAGTATTCCGCTGCGTGAGAAAGGAATGACGATCCATGTGCg                                                                                             >  2:344408/1‑140 (MQ=255)                                 aCCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCggg                                                                                           >  1:186458/1‑140 (MQ=255)                                 aCCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCggg                                                                                           >  1:151882/1‑140 (MQ=255)                                 aCCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCggg                                                                                           <  1:729376/140‑1 (MQ=255)                                  ccAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGt                                                                                          >  1:31337/1‑140 (MQ=255)                                  ccAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGt                                                                                          <  2:181673/140‑1 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGt                                                                                          >  2:600527/1‑138 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGt                                                                                          <  1:600527/138‑1 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  2:588844/1‑140 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  2:666314/1‑140 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  1:803697/1‑140 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  1:413603/1‑140 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  1:556824/1‑140 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  1:136777/1‑140 (MQ=255)                                    aaTCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCa                                                                                        >  1:180393/1‑140 (MQ=255)                                      tCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCAcc                                                                                      <  1:805129/140‑1 (MQ=255)                                        aTAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTg                                                                                    >  1:570689/1‑140 (MQ=255)                                            aGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGgag                                                                                <  2:413603/140‑1 (MQ=255)                                             ggCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGgaga                                                                               >  1:127501/1‑140 (MQ=255)                                                 caTACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAgg                                                                           >  2:142781/1‑140 (MQ=255)                                                 caTACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAgg                                                                           <  2:556824/140‑1 (MQ=255)                                                 caTACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAgg                                                                           >  2:62307/1‑140 (MQ=255)                                                 caTACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAgg                                                                           <  1:62307/140‑1 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    <  2:570689/140‑1 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    >  2:561711/1‑140 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    <  2:745436/140‑1 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    >  2:503746/1‑140 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    >  2:808654/1‑140 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    <  1:561711/140‑1 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    >  2:9638/1‑140 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    >  2:107959/1‑140 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGcc                                                                    <  1:503746/140‑1 (MQ=255)                                                        tCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGAAGATCCATCTGCAGGTCACCTGCGAGAAAGGCGATGcc                                                                    >  1:338181/1‑140 (MQ=255)                                                          aTGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGc                                                                  <  1:808654/140‑1 (MQ=255)                                                            ggCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCAc                                                                <  2:151882/140‑1 (MQ=255)                                                            ggCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCAc                                                                >  2:787242/1‑140 (MQ=255)                                                            ggCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCAc                                                                <  2:726675/140‑1 (MQ=255)                                                            ggCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCAc                                                                <  1:142781/140‑1 (MQ=255)                                                            ggCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATACATGTGCGGGTGACATGGGAGCAAGGCGATGCCGCAc                                                                >  1:291567/1‑140 (MQ=255)                                                             gaaGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  2:201431/138‑1 (MQ=255)                                                             gCAGAATTTCCCGCCTGCTTACTGACTCTCAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  1:344408/140‑1 (MQ=255)                                                             gCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  2:407181/140‑1 (MQ=255)                                                             gCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  1:9638/140‑1 (MQ=255)                                                             gCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  2:110584/140‑1 (MQ=255)                                                             gCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTAAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  1:611417/140‑1 (MQ=255)                                                             gCAGAATTTCCCGCCAGCTTACAGATTCTTAATGGCATAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACg                                                               <  1:294829/140‑1 (MQ=255)                                                                 aaTTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCAGGTGAGGGACACCTGGGAGAAAGGCGATGTCGCACGatat                                                           >  2:318586/1‑140 (MQ=255)                                                                  aTTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATg                                                          <  2:186458/140‑1 (MQ=255)                                                                        cGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAgagg                                                    <  2:617532/140‑1 (MQ=255)                                                                                               gCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCa                             <  1:588844/140‑1 (MQ=255)                                                                                                  aaaGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACg                          <  1:486024/140‑1 (MQ=255)                                                                                                         tGACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTg                   >  1:812124/1‑140 (MQ=255)                                                                                                          gACAATCTACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGc                  <  1:594967/140‑1 (MQ=255)                                                                                                                 tACCCTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCggtggt           <  2:498909/140‑1 (MQ=255)                                                                                                                     cTTGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCg       <  2:31337/140‑1 (MQ=255)                                                                                                                       tGCGCGAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGGAAAGGTGATAGCCGGTGGTGGCGAt     >  1:279289/1‑140 (MQ=255)                                                                                                                         cgcgAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATgg   >  1:790752/1‑140 (MQ=255)                                                                                                                         cgcgAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATgg   <  1:107959/140‑1 (MQ=255)                                                                                                                         cgcgAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATgg   <  1:666314/140‑1 (MQ=255)                                                                                                                         cgcgAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATgg   >  1:373094/1‑140 (MQ=255)                                                                                                                          gcgAAGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGc  <  1:597536/140‑1 (MQ=255)                                                                                                                             aaGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCg       >  1:418124/1‑132 (MQ=255)                                                                                                                             aaGCAATTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCg       <  2:418124/132‑1 (MQ=255)                                                                                                                                  aTTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGc        <  1:421520/126‑1 (MQ=255)                                                                                                                                  aTTATGCCGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGc        >  2:421520/1‑126 (MQ=255)                                                                                                                                         |                                                                                                                             ACCTTTTGTTTTCTGCTCTACACTATCTACAGACCAATCATAAAGGCACATACGATCATGGCAGAATTTCCCGCCAGCTTACTGATTCTTAATGGCAAAAGTACTGACAATCTACCCTTGCGCGAAGCAATTATGCTGTTGCGTGAGGAAGGAATGACGATCCATGTGCGGGTCACCTGGGAGAAAGGCGATGCCGCACGATATGTAGAGGAGGCCCGGAAGTTTGGCGTCGCAACGGTGATTGCCGGTGGTGGCGATGGC  >  NC_000913/2168655‑2168915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑