Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,188,888	T→C	N675D (AAT→GAT)	yehB ←	putative fimbrial usher protein YehB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,188,888	0	T	C	100.0%	190.7 / NA	55	N675D (AAT→GAT)	yehB	putative fimbrial usher protein YehB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/29);  total (26/29)

CTATCGCTTTGCGACACATCCAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATTCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATGCCCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCATTCACTGTCGCAAC  >  NC_000913/2188752‑2189023                                                                                                                                         |                                                                                                                                        cTATCGCTTTGCGACACATCCAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATccat                                                                                                                                      >  1:184332/1‑140 (MQ=255) cTATCGCTTTGCGACACATCCAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATccat                                                                                                                                      <  2:776361/140‑1 (MQ=255)    tCGCTTTGCGACACATCCAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATcac                                                                                                                                   >  2:819938/1‑140 (MQ=255)        tttGCGACACATCCATCATCAGATGATTTTCCCGATATGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCa                                                                                                                               <  1:306485/140‑1 (MQ=255)                  aTCCAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCgg                                                                                                                     >  2:422896/1‑140 (MQ=255)                  aTCCAGCACCAGGTGAGGTTCCCGATAAGGCTTTATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCgg                                                                                                                     <  2:392800/140‑1 (MQ=255)                    ccAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGaa                                                                                                                   >  1:17604/1‑140 (MQ=255)                     cAGCATCAGGTGATTCTCCCGACAACGTGCCATTCCGCCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGaaa                                                                                                                  <  1:233003/140‑1 (MQ=255)                         aTCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACg                                                                                                              >  1:60378/1‑140 (MQ=255)                           cAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGt                                                                                                            >  1:408861/1‑140 (MQ=255)                            aGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGtt                                                                                                           >  1:575519/1‑140 (MQ=255)                            aGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGtt                                                                                                           >  1:578318/1‑140 (MQ=255)                              gTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCg                                                                                                         >  1:775470/1‑140 (MQ=255)                                       ccGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTaa                                                                                                <  2:489557/140‑1 (MQ=255)                                        cGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAAc                                                                                               <  2:131171/140‑1 (MQ=255)                                        cGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAAc                                                                                               <  2:60378/140‑1 (MQ=255)                                        cGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAAc                                                                                               <  2:408861/140‑1 (MQ=255)                                                    aTTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGCTTCTGAATGACGGTTCCCCAGTTTAACGCCACCCGACCa                                                                                   >  2:258482/1‑140 (MQ=255)                                                          tcgtATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGAc                                                                             >  2:633827/1‑140 (MQ=255)                                                          tcgtATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGAc                                                                             >  1:578028/1‑140 (MQ=255)                                                             tataTCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGAGGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAAt                                                                          >  1:393033/1‑140 (MQ=255)                                                               tatCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATGc                                                                        <  2:578318/140‑1 (MQ=255)                                                                     cacTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATGcccctga                                                                  <  1:819938/140‑4 (MQ=255)                                                                       cTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCCCCCAACCAGGCACCAAT‑CCCCCTaaaa                                                               >  1:507226/1‑140 (MQ=255)                                                                         ccATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAaca                                                             >  1:196670/1‑140 (MQ=255)                                                                          cATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGCTTATCGCTACCCGACCTGGCGGCAATG‑CCCCTGAAacac                                                            >  2:323584/1‑140 (MQ=255)                                                                          cATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAacac                                                            <  2:575519/140‑1 (MQ=255)                                                                          cATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGACAGATTAACGCAACCCGACCGGGCGTCAATG‑CCCATGAAacac                                                            >  2:382109/1‑140 (MQ=255)                                                                                ggTTTGTTGTGCGATCTTTTTGGCCGTTGGCATAAGCTTCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTc                                                      <  1:347611/140‑1 (MQ=255)                                                                                  tttGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCa                                                    >  1:85343/1‑140 (MQ=255)                                                                                    tgttgtGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  <  2:184332/140‑1 (MQ=255)                                                                                    tgttgtGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  >  2:203754/1‑140 (MQ=255)                                                                                    tgttgtGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  <  1:639311/140‑1 (MQ=255)                                                                                    tgttgtGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCAGACCAGGGGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  >  1:397623/1‑140 (MQ=255)                                                                                    tgttgtGCGATATTTATGCCCATTGTCATAAGCATCTCTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  <  1:258482/140‑1 (MQ=255)                                                                                     gttgtGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGcc                                                 <  1:419301/140‑1 (MQ=255)                                                                                       tgttccataaTTTTGCCCCTTGGCATAAGCAACTTTAATTCCTTGCGCCTCCCACACCGCAAACGGTTCCGAAAGGCGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTg                                               <  2:507226/134‑1 (MQ=255)                                                                                        gtgCGATATTTTTGCCCATTGATATAAGCATCTTTAACTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGt                                              <  1:323584/140‑1 (MQ=255)                                                                                          gCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  >  1:806833/1‑134 (MQ=255)                                                                                          gCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGc                                                  <  2:806833/134‑1 (MQ=255)                                                                                          gCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCg                                            >  2:824345/1‑140 (MQ=255)                                                                                                tttttGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGt                                      >  1:626477/1‑140 (MQ=255)                                                                                                  tttGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTAc                                    <  2:85343/140‑1 (MQ=255)                                                                                                   ttGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACt                                   <  1:688061/140‑1 (MQ=255)                                                                                                   ttGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACt                                   <  1:824345/140‑1 (MQ=255)                                                                                                      cccATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCga                                <  1:660126/140‑1 (MQ=255)                                                                                                      cccATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCga                                <  2:184956/140‑1 (MQ=255)                                                                                                       ccATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCgac                               <  1:637345/140‑1 (MQ=255)                                                                                                        cATTGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCgact                              <  2:650541/140‑1 (MQ=255)                                                                                                          ttGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCgactga                            >  2:548742/1‑140 (MQ=255)                                                                                                          ttGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCga                                >  1:665765/1‑136 (MQ=255)                                                                                                          ttGACATAAGCATCTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCga                                <  2:665765/136‑1 (MQ=255)                                                                                                                 aaGCATCTGTAATTCCGGGCGCGTCCATCACCGCAAACGATTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAAc                     <  2:397623/140‑1 (MQ=255)                                                                                                                       cTTTAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCAt               >  2:743826/1‑140 (MQ=255)                                                                                                                         ttAATTCCTGGCGCATCCATCACAGTAAAGGTTTCGGCAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCATTc             <  1:320588/140‑1 (MQ=255)                                                                                                                          tAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCATTCa            <  2:666838/140‑1 (MQ=255)                                                                                                                          tAATTCCTGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCATTCa            <  2:775470/140‑1 (MQ=255)                                                                                                                                 tGGCGCATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGATTGACTATAACTGCCATTCACTGTCGc     >  2:504402/1‑140 (MQ=255)                                                                                                                                    cgcATCCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATG‑CCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCATTCACTGTCGCAAc  <  1:743826/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTATCGCTTTGCGACACATCCAGCATCAGGTGATTTTCCCGATAAGGTGTCATTCCGTCGTATATCACCACTCCATTACGGTTTGTTGTGCGATATTTTTGCCCATTGACATAAGCATCTTTAATTCCTGGCGCATTCATCACAGCAAACGTTTCGGAAAGACGGTTCGCCAGATTAACGCCACCCGACCAGGCGACAATGCCCCCTGAAACACTGGCTCCAGCCTGTCGATAAGTACTCGACTGACTATAACTGCCATTCACTGTCGCAAC  >  NC_000913/2188752‑2189023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑