Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,208,064	A→G	D33G (GAC→GGC)	yehP →	VWA domain‑containing protein YehP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,208,064	0	A	G	100.0%	147.6 / NA	42	D33G (GAC→GGC)	yehP	VWA domain‑containing protein YehP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/24);  total (18/24)

AGGCACTGGCATCGCTGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGACAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCAACAGTATTCACAC  >  NC_000913/2207931‑2208199                                                                                                                                      |                                                                                                                                        agGCACTGGCATCGCTGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGc                                                                                                                                   >  2:549400/1‑140 (MQ=255) agGCACTGGCATCGCTGCAAAACTGGGGAGTTTTCCATATCTGAACTGAACGATATTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGc                                                                                                                                   >  2:132042/1‑140 (MQ=255)   gCACTGGCATCGCTGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGccc                                                                                                                                 <  1:606269/140‑1 (MQ=255)   gCACTGGCATCGCTGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGccc                                                                                                                                 <  2:203125/140‑1 (MQ=255)             cGCTGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGa                                                                                                                       >  2:798726/1‑140 (MQ=255)               cTGCAAAACTGGGGAGTTTTCCATGCCTGAACTGAACTATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGAcc                                                                                                                     <  1:277240/140‑1 (MQ=255)                tGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCa                                                                                                                    <  1:20749/140‑1 (MQ=255)                    aaaCTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCAcgcg                                                                                                                <  1:549400/140‑1 (MQ=255)                         ggggAGTTTTCCATGTCCGAACAGAACGATCCTCTAACCCCCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGa                                                                                                           <  2:215291/140‑1 (MQ=255)                             aGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTgg                                                                                                       <  1:798726/140‑1 (MQ=255)                                        tCTGAACTGAACGATGTTCTGAGCAACCGCGAGCTACTACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGgcg                                                                                            <  1:227561/140‑1 (MQ=255)                                        tCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGgcg                                                                                            <  2:537805/140‑1 (MQ=255)                                        tCTGAACTGAACGATCTTCTGACCAACCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGgcg                                                                                            <  1:315811/140‑1 (MQ=255)                                        tCTGAACTGAACGATATTCTGACCACCCGTGAGCTACGACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGgcg                                                                                            <  1:253628/140‑1 (MQ=255)                                           gaactgaacGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGa                                                                                         <  2:44181/140‑1 (MQ=255)                                                 aacGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGTAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGa                                                                                   >  1:43032/1‑140 (MQ=255)                                                 aacGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGa                                                                                   >  1:198711/1‑140 (MQ=255)                                                           tGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAg                                                                         >  2:819048/1‑140 (MQ=255)                                                                accCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCCCGCGCTGGCGTGGCTGAATGGGCGCAATCCAGAACGGCTCCAGCgtgg                                                                    >  2:301056/1‑140 (MQ=255)                                                                accCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCgtgg                                                                    >  2:567808/1‑140 (MQ=255)                                                                  ccGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCgtggtg                                                                  >  2:795772/1‑140 (MQ=255)                                                                  ccGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCAGTAAGGGCGCGAACCTGAACGGCTCCAGCgtggtg                                                                  >  2:271572/1‑140 (MQ=255)                                                                      gAGCTACAACGCTGGTGATTAATTCGTGGGGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACg                                                              <  1:301056/140‑1 (MQ=255)                                                                           aCAACGCTGGCGATTTATTCTTGGCGAAGCGGCAGAAGCGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCg                                                         <  1:271572/140‑1 (MQ=255)                                                                            cAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGAGCGATCCGGAATGGATCCAGCGTGGTGAACGTTCCgg                                                        >  1:273832/1‑140 (MQ=255)                                                                            cAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTACGGGCGCGATCCTGAATGGCTCCAGCGTGGTGAACGTTCCgg                                                        >  1:325461/1‑140 (MQ=255)                                                                                  tGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATTCTGAACGGCTCGAGCGTGGTGCACGTTCCGGTGGAtt                                                  >  1:384416/1‑140 (MQ=255)                                                                                  tGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCAGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGAtt                                                  >  2:323721/1‑140 (MQ=255)                                                                                    gCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATTCTGGAAGGCTCCAGCGCGGTGCACGTTCCGGTGGATTAg                                                >  2:355975/1‑140 (MQ=255)                                                                                            ttCTTGGCGAAGCGGGAGAAACCACTCTTTGTGGGCTTGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCa                                        <  1:251974/140‑1 (MQ=255)                                                                                            ttCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCa                                        <  1:550182/140‑1 (MQ=255)                                                                                                   cGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTca                                 <  1:751901/140‑1 (MQ=255)                                                                                                      aGCGGCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGAGAACGTTACGGGGGTTCAGGTGGCTCAAATCTcacca                              >  1:260798/1‑140 (MQ=255)                                                                                                         ggCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCAccc                           <  1:795772/140‑1 (MQ=255)                                                                                                         ggCAGAAACGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCAccc                           <  1:132042/140‑1 (MQ=255)                                                                                                              aaaCGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAg                      <  1:819048/140‑1 (MQ=255)                                                                                                              aaaCGACGCTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAg                      <  1:563074/140‑1 (MQ=255)                                                                                                                      cTTTGTGGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCaa              <  2:198711/140‑1 (MQ=255)                                                                                                                          gtgGGCTGGATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCAACAGt          >  2:636849/1‑140 (MQ=255)                                                                                                                             ggCTGGATGGCAACGCCCGGCATATAGACCAAGCGCTGGCGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCAACAGTAtt       <  2:212491/140‑1 (MQ=255)                                                                                                                                 ggATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCGAATCTCAGCACGCCAGAGTGGGTCAACAGTATTcaca   >  1:212491/1‑140 (MQ=255)                                                                                                                                  gATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCAACAGTATTCacac  >  1:643164/1‑140 (MQ=255)                                                                                                                                  gATGGCAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCAACAGTATTCacac  <  2:643164/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AGGCACTGGCATCGCTGCAAAACTGGGGAGTTTTCCATGTCTGAACTGAACGATCTTCTGACCACCCGTGAGCTACAACGCTGGCGATTAATTCTTGGCGAAGCGGCAGAAACGACGCTTTGTGGGCTGGATGACAACGCCCGGCAGATAGACCACGCGCTGGAGTGGCTGTATGGGCGCGATCCTGAACGGCTCCAGCGTGGTGAACGTTCCGGTGGATTAGGTGGCTCAAATCTCACCACCCCTGAGTGGATCAACAGTATTCACAC  >  NC_000913/2207931‑2208199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑