Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,548	A→G	L33L (TTA→CTA)	btsS ←	high‑affinity pyruvate receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,548	0	A	G	100.0%	147.8 / NA	42	L33L (TTA→CTA)	btsS	high‑affinity pyruvate receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

TTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCG  >  NC_000913/2214413‑2214675                                                                                                                                        |                                                                                                                                ttACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGt                                                                                                                             <  1:626135/140‑1 (MQ=255)       cgcCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGaat                                                                                                                       >  1:424616/1‑140 (MQ=255)            aTCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATgg                                                                                                                  >  1:47051/1‑140 (MQ=255)                 aCGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGttt                                                                                                             <  2:47051/140‑1 (MQ=255)                  cGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGtttt                                                                                                            <  1:140038/140‑1 (MQ=255)                        ttGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGaataa                                                                                                                     >  2:52346/1‑125 (MQ=255)                        ttGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGaataa                                                                                                                     <  1:52346/125‑1 (MQ=255)                               tAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCAt                                                                                               >  2:676828/1‑140 (MQ=255)                               tAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCAt                                                                                               >  2:557677/1‑140 (MQ=255)                                                       aCCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATc                                                                       >  1:825016/1‑140 (MQ=255)                                                       aCCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTATTAGTCATTAACCATGAAATGACTAAAAAAACGCACATc                                                                       >  2:309529/1‑140 (MQ=255)                                                            gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACAt                                                                        >  2:820004/1‑134 (MQ=255)                                                            gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACAt                                                                        >  2:454351/1‑134 (MQ=255)                                                            gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACAt                                                                        <  1:454351/134‑1 (MQ=255)                                                            gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACAt                                                                        <  1:820004/134‑1 (MQ=255)                                                            gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATctgctg                                                                  >  2:820010/1‑140 (MQ=255)                                                            gTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCAGGCAATGGCTAAAAAAACGCACATctgctg                                                                  >  1:401307/1‑140 (MQ=255)                                                                       gCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCCATGACTAGAAAGACGCACATCTGCTGAAgcagcagca                                                       >  1:235261/1‑140 (MQ=255)                                                                           aagaTGGAAAAGACGATGTAGCCGAGAAATACATGTGGCTGACGAACCTTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAacac                                                   <  2:401307/140‑1 (MQ=255)                                                                           aagaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAacac                                                   <  2:637029/140‑1 (MQ=255)                                                                             gaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGCCTAAAAAAACGCACATATGCTAAAGCAGCAGCAACACCa                                                 >  2:302434/1‑140 (MQ=255)                                                                             gaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCa                                                 >  1:495867/1‑140 (MQ=255)                                                                             gaTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCa                                                 <  2:495867/140‑1 (MQ=255)                                                                              aTGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCTTGCAATGACTAAAAAATCGCACATCAGCTGGAGCAGCAGCAACACCAg                                                >  2:277855/1‑140 (MQ=255)                                                                                ggAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAacac                                                   <  2:431077/135‑1 (MQ=255)                                                                                ggAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAacac                                                   >  1:431077/1‑135 (MQ=255)                                                                                ggAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGAt                                              <  1:676828/140‑1 (MQ=255)                                                                                  aaaaGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTa                                            <  1:806800/140‑1 (MQ=255)                                                                                       aCGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATc                                       >  1:70506/1‑140 (MQ=255)                                                                                          aTGTAGCAGAGAAATCTATGCGCCACACGCACCGTGATCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTa                                    <  1:224053/140‑1 (MQ=255)                                                                                            gTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACa                                  >  2:523174/1‑140 (MQ=255)                                                                                                cAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAATGCACATCTTCTGAAGCGGCAGCAACGCCAGGTTAAAATCGTACATAcc                              >  2:224053/1‑140 (MQ=255)                                                                                                cAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACAt                                 >  2:111480/1‑137 (MQ=255)                                                                                                cAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACAt                                 <  1:111480/137‑1 (MQ=255)                                                                                                    gaAATTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCa                          <  2:825016/140‑1 (MQ=255)                                                                                                     aaaTTTATGCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCaa                         >  1:640824/1‑140 (MQ=255)                                                                                                             gCGGCAGACGAACCGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACt                 <  2:424616/140‑1 (MQ=255)                                                                                                                         ccGTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAAc     <  1:523174/140‑1 (MQ=255)                                                                                                                           gTGACCTGCATTAGCTGTATGAATAATGGTGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAAcgc   <  1:309529/140‑1 (MQ=255)                                                                                                                           gTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAAcgc   <  2:70506/140‑1 (MQ=255)                                                                                                                           gTGACCTGCATTAGCGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCCCAAACCACACTTCCCTTTAAAAcgc   >  1:361093/1‑140 (MQ=255)                                                                                                                           gTGACCTGCATTAGCGGCATGATTAATGGCGGTTTACTCGTTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAAcgc   <  2:262578/140‑1 (MQ=255)                                                                                                                            tGACCTGCATTAGCGGTATGAATAATGGCGTATTACTCAATAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAAcgcg  <  2:235261/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                TTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAACCAGGTGCCCATGATGCAGAAGATGGAAAAGACGATGTAGCAGAGAAATTTATGCGGCAGACGAACCGTGACCTGCATTAACGGTATGAATAATGGCGTTTTACTCATTAACCATGCAATGACTAAAAAAACGCACATCTGCTGAAGCAGCAGCAACACCAGATTAAAATCGTACATACCCGCAAACCACACTTCCCTTTAAAACGCG  >  NC_000913/2214413‑2214675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑