Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,019	T→C	F279L (TTT→CTT)	dld →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,019	0	T	C	100.0%	184.5 / NA	54	F279L (TTT→CTT)	dld	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/24);  total (30/24)

TGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGTTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGCGGAAAAATACGGCA  >  NC_000913/2222889‑2223151                                                                                                                                   |                                                                                                                                     tGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCg                                                                                                                             >  1:29729/1‑140 (MQ=255)             gCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGTGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGc                                                                                                                 <  1:125389/140‑1 (MQ=255)                               cGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGaaa                                                                                               >  2:426245/1‑140 (MQ=255)                               cGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGaaa                                                                                               >  2:207968/1‑140 (MQ=255)                               cGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGaaa                                                                                               >  2:558783/1‑140 (MQ=255)                                 aTCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGTAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATc                                                                                             >  1:481740/1‑140 (MQ=255)                                 aTCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATc                                                                                             >  1:203859/1‑140 (MQ=255)                                 aTCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATc                                                                                             >  1:306340/1‑140 (MQ=255)                                 aTCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATc                                                                                             >  1:414913/1‑140 (MQ=255)                                 aTCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATc                                                                                             >  2:795115/1‑140 (MQ=255)                                 aTCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTGTATATCGTCACCAACCAGCCGGAAGTGCTGACCGAAATc                                                                                             >  2:279557/1‑140 (MQ=255)                                   ccTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATccg                                                                                           <  2:203859/140‑1 (MQ=255)                                       aTCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGt                                                                                       >  2:18708/1‑140 (MQ=255)                                           gTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCata                                                                                   >  2:96673/1‑140 (MQ=255)                                              aTTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTc                                                                                >  2:55880/1‑140 (MQ=255)                                                    aTCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTa                                                                          >  1:809766/1‑140 (MQ=255)                                                     tcttctGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTaa                                                                         >  2:41958/1‑140 (MQ=255)                                                     tcttctGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTaa                                                                         >  1:437195/1‑140 (MQ=255)                                                      cttctGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAAc                                                                        >  2:799052/1‑140 (MQ=255)                                                        tctGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACtt                                                                      >  1:191393/1‑140 (MQ=255)                                                         ctGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTc                                                                     >  1:581867/1‑140 (MQ=255)                                                             ttGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGaaa                                                                 >  2:791004/1‑140 (MQ=255)                                                                   cGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGc                                                           >  2:117309/1‑140 (MQ=255)                                                                          cTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGc                                                    <  1:55880/140‑1 (MQ=255)                                                                            ggCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCg                                                  <  2:414913/140‑1 (MQ=255)                                                                            ggCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCg                                                  <  2:111296/140‑1 (MQ=255)                                                                            ggCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCg                                                  <  2:757432/140‑1 (MQ=255)                                                                               ggTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCgggg                                               <  1:207968/140‑1 (MQ=255)                                                                               ggTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCgggg                                               <  1:18708/140‑1 (MQ=255)                                                                               ggTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCgggg                                               <  2:581867/140‑1 (MQ=255)                                                                               ggTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCgggg                                               <  1:558783/140‑1 (MQ=255)                                                                                gTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGa                                              >  2:566892/1‑140 (MQ=255)                                                                                     tggaGTACGTCTTGATACCTTCGAAGCGGAATAAAATCATCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAatata                                         <  2:301947/137‑1 (MQ=255)                                                                                       cAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATg                                       <  2:768473/140‑1 (MQ=255)                                                                                          tACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCAc                                    <  1:795115/140‑1 (MQ=255)                                                                                           aCGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCAcc                                   >  1:658204/1‑140 (MQ=255)                                                                                            cGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCg                                  >  2:679185/1‑140 (MQ=255)                                                                                              tCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCggg                                <  1:426245/140‑1 (MQ=255)                                                                                              tCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCggg                                <  2:809766/140‑1 (MQ=255)                                                                                              tCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCggg                                <  1:634610/140‑1 (MQ=255)                                                                                              tCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCggg                                <  2:191393/140‑1 (MQ=255)                                                                                                ttGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGat                              <  2:437195/140‑1 (MQ=255)                                                                                                ttGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGat                              <  1:740161/140‑1 (MQ=255)                                                                                                         tCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCGGGTTGCCGGGGGATGTATGCAACGGGATATCTACGat                     >  1:228632/1‑140 (MQ=255)                                                                                                         tCGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGat                     >  1:26743/1‑140 (MQ=255)                                                                                                          cGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCggg                                >  2:640059/1‑128 (MQ=255)                                                                                                          cGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCggg                                <  1:640059/128‑1 (MQ=255)                                                                                                          cGAAGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCAAAAATCTGACGGGTGCAGGGGAATATATGCGCCGGGATATCTACGata                    >  2:290108/1‑140 (MQ=255)                                                                                                             aGCGGAAAAAAATCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTg                 <  1:16424/140‑1 (MQ=255)                                                                                                             aGCGGAAAAAAATCACCAGGTGCTTTATAACGGCACCCACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGATTATATGCACCGGGATATCTACGATATTg                 <  1:279557/140‑1 (MQ=255)                                                                                                                  aaaaaaaTCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGc                <  2:827981/136‑1 (MQ=255)                                                                                                                  aaaaaaaTCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGc                >  1:827981/1‑136 (MQ=255)                                                                                                                         tCAGCAGGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGCGGAAAAATACg     <  1:96673/140‑1 (MQ=255)                                                                                                                            gcagGTGCTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATACACCGGGCTATCTATGATATTGCGGAAAAATACGGCa  >  1:256263/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     TGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCTGGCGGTCTTTGCAGTACGTCTTGATACCTTCGAAGCGGAAAAAAATCAGCAGGTGTTTTATATCGGCACCAACCAGCCGGAAGTGCTGACCGAAATCCGCCGTCATATTCTGGCTAACTTCGAAAATCTGCCGGTTGCCGGGGAATATATGCACCGGGATATCTACGATATTGCGGAAAAATACGGCA  >  NC_000913/2222889‑2223151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑