Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,384	A→G	V169V (GTA→GTG)	sanA →	DUF218 domain‑containing protein SanA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,384	0	A	G	100.0%	168.2 / NA	48	V169V (GTA→GTG)	sanA	DUF218 domain‑containing protein SanA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

GTACGCTGGATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACC  >  NC_000913/2233248‑2233517                                                                                                                                         |                                                                                                                                      gTACGCTGGATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTAtgccgtgccg                                                                                                                                    <  1:626415/140‑1 (MQ=255)        ggATTCCATCGTGCGTACACGCAAAGTTTTCGATTCTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGa                                                                                                                             <  1:686228/140‑1 (MQ=255)          aTTCCATCGTGCGTACACGCAACGTTTTCGATACTAAGGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGaaa                                                                                                                           <  1:403021/140‑1 (MQ=255)                tCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATg                                                                                                                     <  2:764829/140‑1 (MQ=255)                tCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATg                                                                                                                     <  2:437051/140‑1 (MQ=255)                   tGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATgccgtgccgt                                                                                                                                   >  1:182547/1‑123 (MQ=255)                   tGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATgccgtgccgt                                                                                                                                   <  2:182547/123‑1 (MQ=255)                   tGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTg                                                                                                                  >  1:595742/1‑140 (MQ=255)                        acacGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGt                                                                                                             >  1:31946/1‑140 (MQ=255)                                    ttCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAg                                                                                                              <  2:744908/127‑1 (MQ=255)                                    ttCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAg                                                                                                              >  1:744908/1‑127 (MQ=255)                                    ttCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGa                                                                                                 >  1:595229/1‑140 (MQ=255)                                            tAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCAAAAGATATGCTCTCAGTACGTATTCGTGAATTTgccg                                                                                         >  1:352275/1‑140 (MQ=255)                                                     cATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCg                                                                                <  1:142676/140‑1 (MQ=255)                                                     cATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCg                                                                                >  2:173938/1‑140 (MQ=255)                                                         attatCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTgc                                                                            <  2:595742/140‑1 (MQ=255)                                                         attatCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTgc                                                                            <  1:503701/140‑1 (MQ=255)                                                           tatCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTgcgc                                                                          >  2:126011/1‑140 (MQ=255)                                                              cACCCAACGTTTCCATTGTGAGCGATCATCATTTATTTCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCgctgg                                                                       <  1:220864/140‑1 (MQ=255)                                                                cccAACGTTTCCACTGTGAGCGGGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCgctggct                                                                     <  1:389704/140‑1 (MQ=255)                                                                cccAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTgcg                                                                           >  1:41075/1‑134 (MQ=255)                                                                cccAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTgcg                                                                           <  2:41075/134‑1 (MQ=255)                                                                cccAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCgctggct                                                                     <  1:545344/140‑1 (MQ=255)                                                                    aCGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCgctggct                                                                     <  1:641627/136‑1 (MQ=255)                                                                    aCGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCgctggct                                                                     >  2:641627/1‑136 (MQ=255)                                                                     cGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTAAGTACGTAGTCGAGTAAGTGCCGCCCGTTTCAGTGCGCTTGCTGACCt                                                                >  2:389704/1‑140 (MQ=255)                                                                        ttCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTta                                                             <  1:461467/140‑1 (MQ=255)                                                                                tgAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTa                                                     >  1:667573/1‑140 (MQ=255)                                                                                  agcgagcATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTaaa                                                   >  1:54192/1‑140 (MQ=255)                                                                                  agcgagcATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTaaa                                                   >  1:750234/1‑140 (MQ=255)                                                                                          ttatttatTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAAcc                                           >  1:741608/1‑140 (MQ=255)                                                                                          ttatttatTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAAcc                                           >  2:107085/1‑140 (MQ=255)                                                                                                   gcgcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGtttttt                                  >  2:585210/1‑140 (MQ=255)                                                                                                   gcgcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGtttttt                                  >  1:479842/1‑140 (MQ=255)                                                                                                     gcTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAg                                <  1:748248/140‑1 (MQ=255)                                                                                                        gCATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGc                             <  2:479842/140‑1 (MQ=255)                                                                                                         cATATGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGcc                            >  1:405602/1‑140 (MQ=255)                                                                                                          atatGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCg                           <  2:31946/140‑1 (MQ=255)                                                                                                            atGGGGATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCt                         <  2:405602/140‑1 (MQ=255)                                                                                                              ggggATTCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTgg                       >  2:440045/1‑140 (MQ=255)                                                                                                                   ttCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCt                  >  1:129546/1‑140 (MQ=255)                                                                                                                   ttCAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCt                  >  1:461083/1‑140 (MQ=255)                                                                                                                     cAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCt                         <  1:803063/131‑1 (MQ=255)                                                                                                                     cAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCt                         >  2:484453/1‑131 (MQ=255)                                                                                                                     cAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCt                         <  1:484453/131‑1 (MQ=255)                                                                                                                     cAGGCTCAGTGTTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCt                         >  2:803063/1‑131 (MQ=255)                                                                                                                              tgtTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCgg           >  2:537592/1‑136 (MQ=255)                                                                                                                              tgtTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCgg           <  1:537592/136‑1 (MQ=255)                                                                                                                               gtTATGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATg      <  2:750234/140‑1 (MQ=255)                                                                                                                                  aTGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCTTTTTTTAGGGCGGCGGGTCCCGATTCCGGCTATGCAc   >  1:236406/1‑140 (MQ=255)                                                                                                                                  aTGCCGTGCCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCAc   <  1:126011/140‑1 (MQ=255)                                                                                                                                   tgccgtgccgTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGTTGGTCCCTATTCCGGCTATGCAcc  <  2:595229/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTACGCTGGATTCCATCGTGCGTACACGCAAAGTTTTCGATACTAATGATTTCATTATTATCACCCAACGTTTCCACTGTGAGCGAGCATTATTTATTGCGCTGCATATGGGGATTCAGGCTCAGTGTTATGCCGTACCGTCACCGAAAGATATGCTGTCAGTACGTATTCGTGAATTTGCCGCCCGTTTCGGTGCGCTGGCTGACCTTTATATTTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACC  >  NC_000913/2233248‑2233517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑