Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,059	A→G	E9E (GAA→GAG)	preT →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,059	0	A	G	100.0%	207.7 / NA	59	E9E (GAA→GAG)	preT	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/26);  total (33/26)

AATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACT  >  NC_000913/2233928‑2234189                                                                                                                                    |                                                                                                                                   aaTCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCCGGATGAGCTCACTcc                                                                                                                            <  2:60007/140‑1 (MQ=255)       ttCACAGTTTAAATTCCCCCGCACGCTTATCCTTAATGTCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCttt                                                                                                                      <  1:317408/140‑1 (MQ=255)       ttCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCttt                                                                                                                      <  1:718760/140‑1 (MQ=255)       ttCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCttt                                                                                                                      <  1:578426/140‑1 (MQ=255)       ttCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCttt                                                                                                                      <  1:805055/140‑1 (MQ=255)                      cccccGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCAGGCTTTTACGTCTTTACTGGc                                                                                                       >  1:776402/1‑140 (MQ=255)                          cGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCAGATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGAtt                                                                                                   >  2:471307/1‑140 (MQ=255)                                         aaTATCAGTACATTATTATTTGCTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGc                                                                                    <  2:496122/140‑1 (MQ=255)                                         aaTATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGGAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGc                                                                                    >  1:772250/1‑140 (MQ=255)                                         aaTATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCtctc                                                                                      <  1:446647/138‑1 (MQ=255)                                         aaTATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCtctc                                                                                      >  2:446647/1‑138 (MQ=255)                                         aaTATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGc                                                                                    >  1:660222/1‑140 (MQ=255)                                         aaTATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGc                                                                                    <  1:61625/140‑1 (MQ=255)                                               aGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                              >  1:423915/1‑140 (MQ=255)                                               aGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                              >  2:81589/1‑140 (MQ=255)                                               aGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                              >  2:679597/1‑140 (MQ=255)                                               aGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                              >  2:748072/1‑140 (MQ=255)                                               aGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTtta                                                                              <  1:574697/140‑1 (MQ=255)                                                 tACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTttatt                                                                            >  2:702867/1‑140 (MQ=255)                                                      tattattTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTc                                                                       >  2:9639/1‑140 (MQ=255)                                                         tattTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCacg                                                                    >  1:447364/1‑140 (MQ=255)                                                           tttACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCacgac                                                                  >  1:588465/1‑140 (MQ=255)                                                             tACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGc                                                                >  1:782762/1‑140 (MQ=255)                                                                  aaCGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGCACCTTCTCGCTGCTTATCGTGTCACGACGCTCCCt                                                           >  2:354149/1‑140 (MQ=255)                                                                  aaCGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCacgac                                                                  <  1:590059/133‑1 (MQ=255)                                                                  aaCGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCacgac                                                                  >  2:590059/1‑133 (MQ=255)                                                                  aaCGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCt                                                           <  2:811433/140‑1 (MQ=255)                                                                          ccTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAg                                                   <  1:702867/140‑1 (MQ=255)                                                                                 tACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGcc                                            <  1:748072/140‑1 (MQ=255)                                                                                     tataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGc                                        >  1:205822/1‑140 (MQ=255)                                                                                     tataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGc                                        >  2:30382/1‑140 (MQ=255)                                                                                     tataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGc                                        <  1:471307/140‑1 (MQ=255)                                                                                     tataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGc                                        >  1:803154/1‑140 (MQ=255)                                                                                      ataGCATTAAGGAAGATCACATGCCGGAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGca                                       <  2:772250/140‑1 (MQ=255)                                                                                      ataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCGCGACGCTCCCTGTAGTCAGGCTTGCCCGGca                                       <  2:79994/140‑1 (MQ=255)                                                                                      ataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGca                                       <  2:660222/140‑1 (MQ=255)                                                                                      ataGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGca                                       <  2:445174/140‑1 (MQ=255)                                                                                       taGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGcac                                      >  2:572051/1‑140 (MQ=255)                                                                                        aGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGTCTCTCGCTGTTTATTATGTCACGACGCTCCCTTTAGTCAGGCTTGCCCGGcaca                                     >  2:99702/1‑140 (MQ=255)                                                                                        aGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGcc                                            >  1:184707/1‑133 (MQ=255)                                                                                        aGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGcc                                            <  2:184707/133‑1 (MQ=255)                                                                                             tAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCg                                >  2:56842/1‑140 (MQ=255)                                                                                               aGGAAGATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAt                              >  2:183841/1‑140 (MQ=255)                                                                                                    gATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCggg                         >  1:478246/1‑140 (MQ=255)                                                                                                    gATCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATATCACGACGATCCGTGTGGTCAGGCTTGCCCCGCACAGACCGATCCggg                         >  2:270343/1‑140 (MQ=255)                                                                                                    gATCACATGCCGCAACAAAATTATCTGGATGAGCCCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCggg                         >  2:681237/1‑140 (MQ=255)                                                                                                     aTCACATGCCGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCgggg                        >  1:17916/1‑140 (MQ=255)                                                                                                             ccGCAACAAAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTAt                <  2:205822/140‑1 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             <  2:478246/140‑1 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             <  2:782762/140‑1 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             >  1:509932/1‑140 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             >  2:769565/1‑140 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             >  2:697640/1‑140 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGGCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             <  1:490223/140‑1 (MQ=255)                                                                                                                caacaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGGCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCg             >  2:490223/1‑140 (MQ=255)                                                                                                                  acaaAATTATCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCt           <  1:753752/140‑1 (MQ=255)                                                                                                                  acaaAATTATCGGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCt           <  1:56842/140‑1 (MQ=255)                                                                                                                      aaTTATCTGGATGAGTTCACTCCGGTTTTGACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAAt       <  1:354149/140‑1 (MQ=255)                                                                                                                      aaTTATCTGGATGAGGTCACTCGGGATTTTACGTCTTGACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAAt       <  1:270343/140‑1 (MQ=255)                                                                                                                           tCTGGATGAGCTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACt  >  1:680055/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                   AATCTTTTCACAGTTTAAATTCCCCCGCACGCTTAGCCTTAATATCAGTACATTATTATTTACTAAACGCTCGCCTTAATTACCTATAGCATTAAGGAAGATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACT  >  NC_000913/2233928‑2234189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑