Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,269,340 C→T R163C (CGT→TGT)  lpxT → Kdo2‑lipid A phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,269,3400CT97.8% 145.3 / NA 46R163C (CGT→TGT) lpxTKdo2‑lipid A phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/26);  minor base G (0/1);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGCCCAACATTGACTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGT  >  NC_000913/2269205‑2269471
                                                                                                                                       |                                                                                                                                   
gCCAGCCCAACATTGACTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTa                                                                                                                                 >  1:188315/1‑140 (MQ=255)
          cATTGACTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCaaa                                                                                                                       >  2:735696/1‑140 (MQ=255)
           aTTGACTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAg                                                                                                                      <  2:564514/140‑1 (MQ=255)
               aCTTTTACCGATGTTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGc                                                                                                                  >  1:89139/1‑140 (MQ=255)
                cTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCa                                                                                                                 <  2:206963/140‑1 (MQ=255)
                cTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCa                                                                                                                 <  1:65537/140‑1 (MQ=255)
                    tACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCaa                                                                                                                        >  2:500255/1‑129 (MQ=255)
                    tACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCaa                                                                                                                        <  1:500255/129‑1 (MQ=255)
                          tgttAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCg                                                                                                       <  2:89139/138‑1 (MQ=255)
                          tatTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCg                                                                                                       <  1:735696/140‑1 (MQ=255)
                           atTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGc                                                                                                      <  2:619385/140‑1 (MQ=255)
                            ttAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGcc                                                                                                     <  1:580380/140‑1 (MQ=255)
                              aaCCGCGTCAGCGAACTGCTCTATGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCt                                                                                                   <  1:368942/140‑1 (MQ=255)
                                    gTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTtattat                                                                                             >  1:793824/1‑140 (MQ=255)
                                       aGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTAtttt                                                                                          >  2:491626/1‑140 (MQ=255)
                                              tGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAGAGTTGCAAGCCCTATCGCCCTTATTATTTTTGTGGtt                                                                                   >  1:353765/1‑140 (MQ=255)
                                                      gTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCAtt                                                                           >  2:443097/1‑140 (MQ=255)
                                                        tCCCACGAAAGATGCCTCACGATATAGCTTTCCCGTCGATCACGGCATAATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTc                                                                         <  2:211142/140‑1 (MQ=255)
                                                         cccACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTcc                                                                        <  2:56597/140‑1 (MQ=255)
                                                         cccACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTcc                                                                        <  2:17292/140‑1 (MQ=255)
                                                         cccACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTcc                                                                        >  1:405838/1‑140 (MQ=255)
                                                         cccACGAAAGATGCCCCACGAGGTATCTTCCCCGGCGTTCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTcc                                                                        <  1:211602/140‑1 (MQ=255)
                                                          ccACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGACTTTTCGGCCTTATTAGTTTTATGGTTTTTGCATTTccc                                                                       >  2:211602/1‑140 (MQ=255)
                                                           cACGAAAGATGCCTCACGAGATAGCTTTCCCGGGGATCACGGCATGATCCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCa                                                                      <  2:360889/140‑1 (MQ=255)
                                                            aCGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCa                                                                             <  2:762256/132‑1 (MQ=255)
                                                            aCGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCa                                                                             >  1:762256/1‑132 (MQ=255)
                                                             cGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCaga                                                                    <  1:700860/140‑1 (MQ=255)
                                                               aaaGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGt                                                                  >  1:41538/1‑140 (MQ=255)
                                                                 aGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCACTTATTAGTTTGGTGGTTTTTACATTTCCCAGAGTaa                                                                >  1:211142/1‑140 (MQ=255)
                                                                          aCGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGgcg                                                       <  2:634541/140‑1 (MQ=255)
                                                                            gagaTAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGca                                                     <  2:793824/140‑1 (MQ=255)
                                                                            gagaTAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTGTGTGGTTTTTGTATTTCCCAGAGTAATGATTGGCGca                                                     >  1:391293/1‑140 (MQ=255)
                                                                              gaTAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGcaca                                                   <  1:763818/140‑1 (MQ=255)
                                                                                 aGCTTTGCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTTGCGCACACTg                                                >  2:818688/1‑140 (MQ=255)
                                                                                 aGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTg                                                <  2:188315/140‑1 (MQ=255)
                                                                                      tCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCACAGCGTCATGATTGGCGCACACTGGttca                                           >  1:324499/1‑138 (MQ=255)
                                                                                            cGATCACGGCATGATGCCGCTTGTTTTTTCGGCGTCCATGTGGTGTTAGTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGG‑TTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGAcat                                    <  2:353765/140‑1 (MQ=255)
                                                                                              aTCACGGCATGATGCTGCTTATCTTTTCGGCATTCGTGTGGTGTTATTGCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTCACTGAcatc                                   <  2:324499/140‑1 (MQ=255)
                                                                                                             tgctTATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATg                    >  1:524160/1‑140 (MQ=255)
                                                                                                              gctTATTTTTTCGGCCTTCATGTGGGGTTTTTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGa                   <  1:325201/140‑1 (MQ=255)
                                                                                                                 tATTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGTGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCg                >  1:768837/1‑140 (MQ=255)
                                                                                                                  aTTTTTTCGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAAGGATTGGCGCACAGTGGTTTACTGACCTCATTGTCGGTTCGATGACCGt               >  2:325201/1‑140 (MQ=255)
                                                                                                                         cGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGAcc                 >  2:809469/1‑131 (MQ=255)
                                                                                                                         cGGCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGAcc                 <  1:809469/131‑1 (MQ=255)
                                                                                                                          ggCATTCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGTGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGa       <  2:768837/140‑1 (MQ=255)
                                                                                                                          ggCATTCAAGTGGTGTTTTTTCGGCAAAGTGGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGa       <  2:391293/140‑1 (MQ=255)
                                                                                                                               tCATGTGGTGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGt  <  1:491626/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GCCAGCCCAACATTGACTTTTACCGATATTAACCGCGTCAGCGAACTGCTCTCTGTTCCCACGAAAGATGCCTCACGAGATAGCTTTCCCGGCGATCACGGCATGATGCTGCTTATTTTTTCGGCATTCATGTGGCGTTATTTCGGCAAAGTTGCAGGCCTTATCGCCCTTATTATTTTTGTGGTTTTTGCATTTCCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGT  >  NC_000913/2269205‑2269471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑