| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,329,418 | T→C | Q127R (CAA→CGA) | yfaQ ← | tandem DUF2300 domain‑containing protein YfaQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,329,418 | 0 | T | C | 96.2% | 85.8 / NA | 26 | Q127R (CAA→CGA) | yfaQ | tandem DUF2300 domain‑containing protein YfaQ |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (12/13); minor base G (0/1); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGAC > NC_000913/2329299‑2329553 | tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCcac < 2:784864/128‑1 (MQ=255)tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCcac > 1:784864/1‑128 (MQ=255)tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGCTGCCAGTATCGCCCCCACACATCTGCGCCa > 1:508052/1‑140 (MQ=255) tAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCAAGTATCGCCCCCCCACGTCTGCGCCCATATACAACCGCTGCGGCGCaa > 2:349815/1‑140 (MQ=255) aaTGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCaaa < 2:39854/140‑1 (MQ=255) aTGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCaaaa < 1:635458/140‑1 (MQ=255) aTCAAGCAGCGATTTCACCGTTTCCCAGGCTTCTGGCCTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga < 1:218428/140‑1 (MQ=255) tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGCTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATagag < 2:508052/140‑1 (MQ=255) aaGCAGCGATTTAACCGTTACCGAGGTTTGTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGcc < 1:349815/140‑1 (MQ=255) agcagcGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga > 1:34851/1‑136 (MQ=255) agcagcGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga < 2:34851/136‑1 (MQ=255) gcagcGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCca > 2:735783/1‑140 (MQ=255) aCCGTTACCGAGGTTTCTGGCTCCACCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTAc < 1:305481/140‑1 (MQ=255) tACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAgcg > 1:767318/1‑140 (MQ=255) tACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAgcg < 2:576998/140‑1 (MQ=255) aCCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAAGATGCAACCACTGGGGCCCAAAATAGCGCCCACAGGAACGTACCAgcgc > 2:305481/1‑140 (MQ=255) ccGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGcgcg < 1:22793/140‑1 (MQ=255) gTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATc < 2:467116/140‑1 (MQ=255) cAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGGCCACAGGGACGGACGAGCGCGGTATCGCGCGTTATGc > 1:230228/1‑140 (MQ=255) aGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct > 2:764422/1‑140 (MQ=255) aGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGc > 2:2381/1‑139 (MQ=255) aGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGc < 1:2381/139‑1 (MQ=255) agaCGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGGACCAGCGTGGTATCGCGCGTTATGCTCTCCCCAGCCt > 1:309006/1‑140 (MQ=255) cGCTAACCACGCAGGGGCCTGACGCGGTTGCCAGTATGGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac < 1:285044/140‑1 (MQ=255) gCAGGTGCCTGACGCTGTTGCCAGTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAAc > 1:482554/1‑140 (MQ=255) gTATCGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGac < 1:735783/140‑1 (MQ=255) | TGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTCCGCGAC > NC_000913/2329299‑2329553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |