Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,352,275	T→C	I34V (ATA→GTA)	glpT ←	sn‑glycerol 3‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,352,275	0	T	C	100.0%	178.5 / NA	50	I34V (ATA→GTA)	glpT	sn‑glycerol 3‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

ATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTAT  >  NC_000913/2352154‑2352402                                                                                                                          |                                                                                                                                aTCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCa                                                                                                               >  1:678568/1‑140 (MQ=255)  tCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAg                                                                                                              >  2:590449/1‑140 (MQ=255)       aTCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCaa                                                                                                         >  2:798722/1‑140 (MQ=255)         ccccGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATc                                                                                                       <  1:612432/140‑1 (MQ=255)         ccccGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATc                                                                                                       <  1:798722/140‑1 (MQ=255)               aaGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGAt                                                                                                 <  2:589866/140‑1 (MQ=255)                aGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATa                                                                                                >  2:492/1‑140 (MQ=255)                   gCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGt                                                                                             >  1:674984/1‑140 (MQ=255)                                 aCCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcg                                                                               <  1:575195/140‑1 (MQ=255)                                    gcgTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCgg                                                                            >  1:512355/1‑140 (MQ=255)                                       tGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAg                                                                         >  1:97340/1‑140 (MQ=255)                                                  gCTCAACCAGATAAGTCATAGCAAGTGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              <  1:344322/140‑1 (MQ=255)                                                  gCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              <  1:492/140‑1 (MQ=255)                                                  gCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              <  1:28245/140‑1 (MQ=255)                                                  gCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              <  2:633131/140‑1 (MQ=255)                                                  gCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              >  2:540636/1‑140 (MQ=255)                                                  gCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              <  2:816270/140‑1 (MQ=255)                                                    tCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCACAATCGACCATACGTCCGATCGATCACCGCGGCAGGTAAGCGCGCttt                                                            >  2:255548/1‑140 (MQ=255)                                                       aCCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGTCAGCGCAATCGACGATAATTCGGATCAATCACCGCGGCAGCTAAGCGCGCTTtgtg                                                         >  1:337324/1‑140 (MQ=255)                                                        ccAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              <  1:714489/134‑1 (MQ=255)                                                        ccAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCt                                                              >  2:714489/1‑134 (MQ=255)                                                        ccAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTtgtgt                                                        >  2:508539/1‑140 (MQ=255)                                                            aTAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcg                                                    <  2:97340/140‑1 (MQ=255)                                                             tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                                   <  2:104819/140‑1 (MQ=255)                                                             tAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                                   <  1:115367/140‑1 (MQ=255)                                                                ggCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATCAGTCGGATGGATCTCCACGGCAGGTAAGCGCGCTTTGTGTGGCGCTgg                                                >  2:343235/1‑140 (MQ=255)                                                                ggCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGGTCTCCGCGGCAGGTAAGCGCGTTTTGTGTGGCGCTgg                                                >  1:391530/1‑140 (MQ=255)                                                                  cATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTTCGCGGCAGGTAAGCGCGATTTGTGTGGCGCTGGtt                                              >  2:247141/1‑140 (MQ=255)                                                                  cATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGtt                                              >  2:460567/1‑140 (MQ=255)                                                                      gCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaaa                                          >  1:663796/1‑140 (MQ=255)                                                                      gCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaaa                                          >  2:530575/1‑140 (MQ=255)                                                                       cAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTaaaa                                         <  1:558195/140‑1 (MQ=255)                                                                           cgcAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATAc                                     <  2:678568/140‑1 (MQ=255)                                                                           cgcAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATAc                                     <  1:37732/140‑1 (MQ=255)                                                                               aaGTTCTTACGAACCGAATAGTGAGTCGCGTAGCCAAGGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCaa                                 <  1:343235/140‑1 (MQ=255)                                                                               aaGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCaa                                 <  1:530575/140‑1 (MQ=255)                                                                               aaGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCaa                                 <  1:540636/140‑1 (MQ=255)                                                                                aGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCGGCGGCAGGCAAGAGGGGTTTGTGTGCCGCTGGGTTAAAATTACTCAAc                                >  2:380786/1‑140 (MQ=255)                                                                                     ttACGAACCAAATAGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGa                           >  2:628208/1‑140 (MQ=255)                                                                                                  aGTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGc              <  1:440809/140‑1 (MQ=255)                                                                                                   gTAAGCCGCATAGCCAGAGAATTCCCCCAGTTACAGTTGTCAGCGCAAGCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGcc             <  2:391530/140‑1 (MQ=255)                                                                                                   gTAAGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGcc             <  1:812179/140‑1 (MQ=255)                                                                                                     aaGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCg           <  2:663796/140‑1 (MQ=255)                                                                                                      aGCCGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGt          <  1:142159/140‑1 (MQ=255)                                                                                                         cGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGt       >  1:764602/1‑140 (MQ=255)                                                                                                         cGCATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTAGCGCTGGTATAAAAATTCTCAACATAGATAGCCTCCGTGGCCCGTggg       >  2:388526/1‑139 (MQ=255)                                                                                                           cATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGTGCTGGTTTCAAACTACGCAACATTGCTAGCCTCCGTGGCCCGTGGTCt     >  2:322582/1‑140 (MQ=255)                                                                                                           cATAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCt     >  2:84583/1‑140 (MQ=255)                                                                                                            aTAGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAATATACTCAACATTGAAAGCCTCAGTGGCCCGTGGTCtt    >  1:318995/1‑140 (MQ=255)                                                                                                              aGCCAAAGAATCCCCGCAGGAACATATGCCAGCGCAAACGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCtcat  <  1:388526/140‑1 (MQ=255)                                                                                                              aGCCAAAGAATACCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCttat  <  1:508539/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                ATCGAGATCCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTAT  >  NC_000913/2352154‑2352402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑