Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,361,874	T→C	Y113C (TAC→TGC)	yfaX ←	putative DNA‑binding transcriptional regulator YfaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,874	0	T	C	100.0%	88.0 / NA	26	Y113C (TAC→TGC)	yfaX	putative DNA‑binding transcriptional regulator YfaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

CCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGTAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCA  >  NC_000913/2361736‑2362003                                                                                                                                           |                                                                                                                                  ccTTCGATAATACTTTGCTGT‑CCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGcaa                                                                                                                                 >  1:493433/1‑140 (MQ=255)     cGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCGGATAGTGACTGATGGCTCCACATTCAAAATGcaataa                                                                                                                              >  1:227977/1‑140 (MQ=255)           taCTTTGCTGTACCGCTGCAGGTTGCCAGGCTAGCCGGCCTTTACCTACTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTg                                                                                                                        <  2:227977/140‑1 (MQ=255)           taCTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTg                                                                                                                        <  1:623046/140‑1 (MQ=255)                tGCTGTACCGCTGCACGTTGCCCGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGtt                                                                                                                   <  1:210098/140‑1 (MQ=255)                          cTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTg                                                                                                                        <  1:603509/125‑1 (MQ=255)                          cTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTg                                                                                                                        >  2:603509/1‑125 (MQ=255)                          cTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCAGATGACTCGACTCTCAAAATGCAATAAGCGCTGCCGTTATCAATAATc                                                                                                         >  2:404551/1‑140 (MQ=255)                              aGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCa                                                                                                     <  1:111353/140‑1 (MQ=255)                                  tGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAGAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTg                                                                                                 >  1:134324/1‑140 (MQ=255)                                   gCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAGAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGa                                                                                                <  2:134324/140‑1 (MQ=255)                                   gCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAACGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGa                                                                                                <  2:359655/140‑1 (MQ=255)                                             cAGGCATTTACCTATGCCGGAGTGAGAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGcc                                                                                      <  1:404551/140‑1 (MQ=255)                                                                     aTAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCa                                                              >  2:729916/1‑140 (MQ=255)                                                                              aaGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTc                                                     >  2:605665/1‑140 (MQ=255)                                                                                      ttCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGt                                             <  2:493433/140‑1 (MQ=255)                                                                                           tCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCg                                        >  2:18501/1‑140 (MQ=255)                                                                                           tCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCg                                        <  1:729916/140‑1 (MQ=255)                                                                                                     gcacgcTGATAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATc                              <  2:818003/140‑1 (MQ=255)                                                                                                              tAGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGATGCGTCAAACGCCGTCGAGCCAGTTCCCGGCGATCCATCTTGCTTAAc                     >  2:300137/1‑140 (MQ=255)                                                                                                               aGTGGCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACg                    <  1:18501/140‑1 (MQ=255)                                                                                                                   gCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATg                >  2:162634/1‑140 (MQ=255)                                                                                                                   gCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATg                >  2:409683/1‑140 (MQ=255)                                                                                                                   gCTGATGACTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATg                >  2:612860/1‑140 (MQ=255)                                                                                                                     tgatgaCTCCACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGAc              >  2:614671/1‑140 (MQ=255)                                                                                                                             ccACTTTCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGGCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGc      >  2:291165/1‑140 (MQ=255)                                                                                                                                 tttCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTcca  <  1:605665/140‑1 (MQ=255)                                                                                                                                 tttCAAAATGCAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTcca  <  1:409683/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                  CCTTCGATAATACTTTGCTGTACCGCTGCAGGTTGCCAGGCAAGCAGGCATTTACCTATTCCGGAGCGATAAAGCGAAAGGCTTTTTCCTTCATGGGAACGCACGCTGATAGTGGCTGATGACTCCACTTTCAAAATGTAATAAGCGCTGCCGTTATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCA  >  NC_000913/2361736‑2362003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑