| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 300,444 | T→C | D164G (GAC→GGC) | paoC ← | aldehyde dehydrogenase: molybdenum cofactor‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 300,444 | 0 | T | C | 97.7% | 141.8 / NA | 44 | D164G (GAC→GGC) | paoC | aldehyde dehydrogenase: molybdenum cofactor‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (23/20); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTCGCGCCTGTTCGAAGGT > NC_000913/300309‑300580 | gAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCACATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCAcc < 1:162263/140‑1 (MQ=255) gTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGAc > 2:752570/1‑140 (MQ=255) cAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCAGCGCCGCGGAGGTGAAAGCCCCTTCAAAGCCACCGACGtt > 2:273213/1‑140 (MQ=255) gCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGc < 1:123750/140‑1 (MQ=255) gCTTATTTCCATCCCAGACGGCAATCGAGACATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGc < 1:372236/140‑1 (MQ=255) tttCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTc > 2:734204/1‑140 (MQ=255) tttCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTc > 2:435665/1‑140 (MQ=255) tttCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTc > 1:726731/1‑140 (MQ=255) gACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCcggcggtt < 1:752570/140‑3 (MQ=255) aTCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGAc > 2:744094/1‑140 (MQ=255) cGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACgg > 1:492973/1‑140 (MQ=255) cGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACgg > 1:520779/1‑140 (MQ=255) cTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGt < 1:435665/140‑1 (MQ=255) ccATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTcc > 1:476152/1‑140 (MQ=255) cATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTATCTTCCGGCGGCTGGTTGACGACCTGTTTTTCGTCCg > 1:345511/1‑140 (MQ=255) cATCGCCATATGGCTCTGGTCCGGGGCCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTGTGTCGGGCGTGTATTCGGGCGGCTGTTTGACGGCCTGTTTTTCGTccc > 2:298722/1‑139 (MQ=255) aTCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGc > 1:790689/1‑140 (MQ=255) cGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCa < 2:726731/140‑1 (MQ=255) cGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCa < 2:37403/140‑1 (MQ=255) ccATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCTGGCGTGTCCTCCTGCGGCTGATTGAGGGCCTGTTTTTCGTCCGCCAgg > 2:247588/1‑140 (MQ=255) ggCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTaa < 2:492973/140‑1 (MQ=255) ggCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTaa < 2:520779/140‑1 (MQ=255) gCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAg < 2:162033/140‑1 (MQ=255) gCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAg < 1:734204/140‑1 (MQ=255) ctctGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGc > 1:93637/1‑140 (MQ=255) ctctGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGc > 1:574854/1‑140 (MQ=255) ctctGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCA‑GGAGTAAGCt > 2:704685/1‑140 (MQ=255) ctGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTc < 2:591128/140‑1 (MQ=255) tCCGGGGTCGTGTAGATAGCATTAAGCTTTACCGCAGGGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCttt < 1:344999/140‑1 (MQ=255) cGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTttat < 2:93637/140‑1 (MQ=255) cGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTttat < 2:574854/140‑1 (MQ=255) ggtagCATCAATCTTTACCGCACCGGAGGGGAAAGCCACGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAgt < 1:275375/140‑1 (MQ=255) agCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGtgtg > 2:463341/1‑140 (MQ=255) cATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGTCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGcc > 1:360610/1‑140 (MQ=255) tCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGAGAGTAAGATCCTTTATTACGGGGATAGTGTGCCTGCAcc > 2:211715/1‑140 (MQ=255) aGCGGAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGgccg > 1:650577/1‑140 (MQ=255) gAGGTGAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCcgccgc > 2:433202/1‑140 (MQ=255) gAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTcgcg < 1:433202/140‑1 (MQ=255) gAAAGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCA‑GGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTCgcgc < 1:704685/140‑1 (MQ=255) aaaGCCCCGTCAAAGCCGCCGAAGTTTTTGTCGGGCGTGGCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTCgcgc < 2:345511/140‑1 (MQ=255) aaaGCCCCGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTCgcgc > 2:692857/1‑140 (MQ=255) cccGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTcg > 2:33494/1‑132 (MQ=255) cccGTCAAAGCCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTcg < 1:33494/132‑1 (MQ=255) aaGCCACCGACGTTTTTGTCGGCCGCGTCGTCCGGCGCCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTCGCGCCTGTTCGAAGGt < 1:211715/140‑1 (MQ=255) | GAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATTGACGGCCTGTTTTTCGTCCGCCAGGGAGTAAGCTCCTTTATTACGGCGATAGTGTGCCTGCACCAGCGAGGCCGCCGCTCGCGCCTGTTCGAAGGT > NC_000913/300309‑300580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |