Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,408,713	G→A	V385I (GTC→ATC)	alaA →	glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,408,713	0	G	A	100.0%	93.7 / NA	29	V385I (GTC→ATC)	alaA	glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

GCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTGTCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCGGAGAT‑TGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATA  >  NC_000913/2408578‑2408849                                                                                                                                        |                                                                                                                                           gCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCga                                                                                                                                        <  1:717442/140‑1 (MQ=255)       cGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGatat                                                                                                                                  <  2:191905/140‑1 (MQ=255)           ttAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAg                                                                                                                              <  2:778782/140‑1 (MQ=255)           ttAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAg                                                                                                                              <  1:495799/140‑1 (MQ=255)                 ttCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGGTACGGCGTATCGAGGATATCGAGCTGTCt                                                                                                                        >  1:322704/1‑140 (MQ=255)                      gacgaTCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAg                                                                                                                   >  1:51831/1‑140 (MQ=255)                            cAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGtt                                                                                                             >  2:714292/1‑140 (MQ=255)                                aaaTGGTGTTGGATTTCCTGTTGCAGGAATAAGTTCTTTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTcgcg                                                                                                         <  1:316926/140‑1 (MQ=255)                                aaaTGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTcgcg                                                                                                         <  2:51831/140‑1 (MQ=255)                                   tGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGt                                                                                                      <  1:134278/140‑1 (MQ=255)                                      tgtTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGCCGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTc                                                                                                   >  1:787813/1‑140 (MQ=255)                                      tgtTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTc                                                                                                   >  2:452912/1‑140 (MQ=255)                                       gtTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTcc                                                                                                  >  1:655588/1‑140 (MQ=255)                                       gtTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTcc                                                                                                  <  1:817697/140‑1 (MQ=255)                                        ttGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGACTGTGAGCATGTTCGCGCGTTTCCt                                                                                                 >  1:378734/1‑140 (MQ=255)                                         tGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCtt                                                                                                <  2:86758/140‑1 (MQ=255)                                          ggATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCttt                                                                                               <  2:697929/140‑1 (MQ=255)                                           gATTTCCTATTGCAGGAAAAAGGTCTGTTGGGGCAGGGGGCGGCATTCTACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTc                                                                                              <  2:378734/140‑1 (MQ=255)                                              ttCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTgg                                                                                           <  2:99197/140‑1 (MQ=255)                                                ccTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGtt                                                                                         >  1:685370/1‑140 (MQ=255)                                                                         gTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCa                                                               <  1:459480/140‑1 (MQ=255)                                                                          tGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCAc                                                              >  2:153510/1‑140 (MQ=255)                                                                             aaGGGACGGCATTCAGCTGGCCGTGCCCGCATCACTTACGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGATCAAGTTCGCGCGTATCCTTTCTGGTTATCATCAGCTGTA‑ATCGTAATTTCACTGc                                                           <  1:272474/140‑1 (MQ=255)                                                                             aaGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGc                                                           <  2:421784/140‑1 (MQ=255)                                                                                ggACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTGTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCgg                                                        >  1:335335/1‑140 (MQ=255)                                                                                   cGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCGgaga                                                     >  2:780010/1‑140 (MQ=255)                                                                                                                        tGTCACGCTACCGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCGGAGAT‑TGCATCCGGCAGCTTTATCCCGCCACAATGACCtga               <  1:714292/140‑1 (MQ=255)                                                                                                                            ccGCTACCGCGTATCGATGATATCGAGCTGTGTTTAAGCCAGTTCGCGCGTTTCCTTTCTGGTTATCACCAGCTGTA‑ATCTTAATTTCACTGCCGGAGAT‑TGCATCCGGCAGCGTTATCCCGCCACAATGACCTgatgat           <  2:335335/139‑1 (MQ=255)                                                                                                                            ccGCTACCGCGTATCGACGATATCGAGCTGTTTTTTAGCAAGTTCGCGTGTTTCCTTTCTGTTTATCATCAGCTGTA‑ATCTTAATTTCCCTGCCGGAGAT‑TGCATCCGGCAGCGTTATCCCGCCACAATGACCTgatgat           <  2:345013/139‑1 (MQ=255)                                                                                                                             ggCTACCGCGTATCGATGATATCGAGTTGTTTTTAAGCAAGTTCGCGGGTTTCTTTTCTGGTTATCATCAGCTGTA‑ATTTTAATTTCCCTGCCGGAGAT‑TGCATCGGGCCGCGTTCTCCCGCCACAATGACCTGatgatg          <  2:451186/139‑1 (MQ=255)                                                                                                                                  ccGCGTATCGATGATATCGAGTTGTTTTTGAGCAAGTTCGCGCTTTTCCTTTCAGGTTATCATCAGCTTTA‑ATCTTAATTTCACTGCGGGAGAT‑TGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGtcatc     <  2:685370/140‑1 (MQ=255)                                                                                                                                  ccGCGTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCGGAGAT‑TGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGtcatc     >  1:677925/1‑140 (MQ=255)                                                                                                                                    gcgTATCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCGGAGAT‑TGCATCCGGCGGCGTTATCCCGACACAATGACATGATGATGTcatcat   >  1:352313/1‑140 (MQ=255)                                                                                                                                     cgTTTCGATGATATCGGGCTGTCTTGGAGCAAGTGCGCGCGTTTCCTTTGTGGTTCTC‑‑CAGCGTTAGATGTTTATTTGAGCCGGGGGGATGTGGACCAGGCATTCTTCTGCCGCCACAATGACCTGATGATGTCATCATa  <  2:787813/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                           GCCAAACGCTTTAACATTCACGACGATCAGAAAATGGTGTTGGATTTCCTGTTGCAGGAAAAAGTTCTGTTGGTGCAAGGGACGGCATTCAACTGGCCGTGGCCGGATCACTTCCGCATTGTCACGCTACCGCGTGTCGATGATATCGAGCTGTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTA‑ATCTTAATTTCACTGCCGGAGAT‑TGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATA  >  NC_000913/2408578‑2408849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑