Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,412,679 A→G *152Q (TAA→CAA)  yfbV ← UPF0208 membrane protein YfbV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,6790AG98.1% 174.1 / NA 52*152Q (TAA→CAA) yfbVUPF0208 membrane protein YfbV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (30/21);  minor base C (0/1);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTT  >  NC_000913/2412544‑2412810
                                                                                                                                       |                                                                                                                                   
catcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGAATGACTTCTTTTATATGCGTAATCAGGGGGCAATTGCaaa                                                                                                                                 >  2:30920/1‑140 (MQ=255)
    ttGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAatcat                                                                                                                             <  2:144181/140‑1 (MQ=255)
      gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAAtcatca                                                                                                                           >  2:682048/1‑140 (MQ=255)
         cTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCa                                                                                                                                   >  1:590571/1‑129 (MQ=255)
         cTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAgg                                                                                                                        >  2:643191/1‑140 (MQ=255)
          tGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGa                                                                                                                       <  1:682048/140‑1 (MQ=255)
                 aCAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGa                                                                                                                       >  1:501140/1‑133 (MQ=255)
                 aCAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGa                                                                                                                       <  2:501140/133‑1 (MQ=255)
                    aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATc                                                                                                             <  2:79451/140‑1 (MQ=255)
                    aaaTAAGACGTTGCGCGTTGGTCATTTCCATCGTTTACTACTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATc                                                                                                             <  2:333644/140‑1 (MQ=255)
                     aaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATAAGGGGTCGATTGCATATCATCCAGGAAAGTTTTATcc                                                                                                            >  1:404068/1‑140 (MQ=255)
                          gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAGAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGa                                                                                                                       <  2:492394/124‑1 (MQ=255)
                          gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAGAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGa                                                                                                                       >  1:492394/1‑124 (MQ=255)
                               tGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGAAAAGGTTTATCCAGTTGTTTGa                                                                                                  >  2:350966/1‑140 (MQ=255)
                                 cgcgTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAg                                                                                                >  2:533634/1‑140 (MQ=255)
                                             ttCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAAGAAAGTTTTATACATTTGTTTTAAGGCGCGCTTAAGc                                                                                    >  1:333548/1‑140 (MQ=255)
                                                       gACTCCTGTATCACTCTCCTACCGTGAAAAAGAAGCAGGGTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTa                                                                          <  2:234959/140‑1 (MQ=255)
                                                       gACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTTTCCAGTTGTTTGAAGGCGCGCCTAAGCGTGTCAGCTa                                                                          >  1:234959/1‑140 (MQ=255)
                                                       gACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTa                                                                          <  2:421440/140‑1 (MQ=255)
                                                       gACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTa                                                                          >  1:421440/1‑140 (MQ=255)
                                                         cTCCTGTATCACTCTACTACGGGGAAAAAAAAGAATGCTGAGTATGCCGTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAAt                                                                        <  2:333548/140‑1 (MQ=255)
                                                               tATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTgg                                                                  >  1:559698/1‑140 (MQ=255)
                                                                    ctctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATTCAGTTGTTTGAAGGCGGGCTTAAGCCTGTCAGCTAATGCCTGGTAATc                                                             >  2:359291/1‑140 (MQ=255)
                                                                    ctctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATc                                                             >  1:627880/1‑140 (MQ=255)
                                                                    ctctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATc                                                             >  2:559153/1‑140 (MQ=255)
                                                                    ctctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATc                                                             >  2:100815/1‑140 (MQ=255)
                                                                      ctactaCGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAg                                                           >  2:567043/1‑140 (MQ=255)
                                                                       tactacGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg                                                          >  1:148020/1‑140 (MQ=255)
                                                                        actacGGTGAAAAAAACGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGc                                                         <  2:499128/140‑1 (MQ=255)
                                                                        actacGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGc                                                         <  1:700210/140‑1 (MQ=255)
                                                                               tGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCtt                                                  >  1:825874/1‑140 (MQ=255)
                                                                                 aaaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAATCGTGTCAGCTATTGACTGGTAGTCAGGCTTGCCTTCa                                                >  1:214592/1‑140 (MQ=255)
                                                                                 aaaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCa                                                >  1:155719/1‑140 (MQ=255)
                                                                                    aaaaaGAAGGCTGAGTCTGCCTTCTTTTATTGGCGTAATGAGGGGTCACTTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACg                                             <  2:404068/140‑1 (MQ=255)
                                                                                    aaaaaGAAGGCTGAGGATGCCTTCTTTTATATGCGTACTCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACg                                             <  1:350966/140‑1 (MQ=255)
                                                                                         gaagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGc                                        >  1:538838/1‑140 (MQ=255)
                                                                                          aagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGcc                                       <  1:643191/140‑1 (MQ=255)
                                                                                              cTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAaca                                   <  1:559153/140‑1 (MQ=255)
                                                                                               tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac                                  <  2:825874/140‑1 (MQ=255)
                                                                                               tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac                                  <  2:627880/140‑1 (MQ=255)
                                                                                               tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAacac                                  <  2:180434/140‑1 (MQ=255)
                                                                                                 aGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGACAGCTAATTCCTGGTAATCAGGCTTACCTTGAACGGGTGCCAACACCt                                >  2:338568/1‑140 (MQ=255)
                                                                                                    aTGCCTTCTTTTATATGCGTAAACAGGGGTCGATTCCAAGTCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTc                             <  2:310356/140‑1 (MQ=255)
                                                                                                         ttcttTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGAc                        <  2:148020/140‑1 (MQ=255)
                                                                                                           cttTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCACCACCTGTCCAGACTc                      >  1:310356/1‑140 (MQ=255)
                                                                                                           cttTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTc                      >  1:55867/1‑140 (MQ=255)
                                                                                                           cttTTATATGCGTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTc                      >  1:466408/1‑140 (MQ=255)
                                                                                                                      gTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTAc           >  2:151568/1‑140 (MQ=255)
                                                                                                                      gTAATCAGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTAc           >  1:39798/1‑140 (MQ=255)
                                                                                                                          tCAGGGGTCAATTGCAACTCATCAAGGAAAGTTTTCTCCTGTTGTTTGCAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACcgcg       <  2:214592/140‑1 (MQ=255)
                                                                                                                            aGGGGTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGaa     <  2:155719/140‑1 (MQ=255)
                                                                                                                              gggTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGGCAACACCGGTCAAGACTCCTGCAATTTACCGCGAACt   >  1:316671/1‑140 (MQ=255)
                                                                                                                               ggTCAATTGCAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACtt  >  1:671883/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGCTTGCCTTCAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTT  >  NC_000913/2412544‑2412810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑