Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,285	C→T	A34T (GCA→ACA)	mepA ←	peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,285	0	C	T	100.0%	89.4 / NA	29	A34T (GCA→ACA)	mepA	peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

GGATAAACATCACCAGATCCGGGTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGCGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTT  >  NC_000913/2446150‑2446411                                                                                                                                        |                                                                                                                               ggATAAACATCACCAGATCCGGGTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTa                                                                                                                            >  1:15661/1‑140 (MQ=255)  gATAAACATCACCAGATCCGGGTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTAc                                                                                                                           >  1:606458/1‑140 (MQ=255)              cAGATCCGGGTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGtt                                                                                                               >  1:175928/1‑140 (MQ=255)                 aTCCGGGTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTggg                                                                                                            <  2:774216/140‑1 (MQ=255)                  tCCGGGTGACCGAAATAACGACTCTGATCGGAACGCATGTCCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGt                                                                                                           <  2:220491/140‑1 (MQ=255)                      ggTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTAtt                                                                                                       >  2:126860/1‑140 (MQ=255)                           ccGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTg                                                                                                  >  2:23755/1‑140 (MQ=255)                                                     cATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGgcgc                                                                        <  2:175928/140‑1 (MQ=255)                                                           cTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTgg                                                                  >  1:550668/1‑140 (MQ=255)                                                           cTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTgg                                                                  >  2:540938/1‑140 (MQ=255)                                                           cTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTgg                                                                  <  1:126860/140‑1 (MQ=255)                                                                                   cAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATcg                                          >  1:194522/1‑140 (MQ=255)                                                                                       gtgtCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGGGCTACTGGCAAGCCGAGCCAGCAGCGCAATCGCggg                                      >  2:450267/1‑139 (MQ=255)                                                                                       gtgtCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGt                                      >  1:69044/1‑140 (MQ=255)                                                                                         gtCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATcg                                          >  2:152722/1‑134 (MQ=255)                                                                                         gtCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATcg                                          <  1:152722/134‑1 (MQ=255)                                                                                             gcgcCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTAtt                                <  1:540938/140‑1 (MQ=255)                                                                                                       tACAGCCATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTAc                      <  2:606458/140‑1 (MQ=255)                                                                                                            ccATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTTCCAGCg                 <  2:583197/140‑1 (MQ=255)                                                                                                            ccATTAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCg                 >  2:176655/1‑140 (MQ=255)                                                                                                               ttAGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTgg              >  2:22388/1‑140 (MQ=255)                                                                                                                 aGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTg               >  1:725182/1‑137 (MQ=255)                                                                                                                 aGAAAAACTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTg               <  2:725182/137‑1 (MQ=255)                                                                                                                   aaaaaCTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAata          <  2:701819/140‑1 (MQ=255)                                                                                                                    aaaaCTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAatat         <  2:15661/140‑1 (MQ=255)                                                                                                                    aaaaCTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAatat         <  2:550668/140‑1 (MQ=255)                                                                                                                    aaaaCTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAatat         >  2:753734/1‑140 (MQ=255)                                                                                                                      aaCTGCCTATCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAAACGCGGTTTTATACAATTTTTAGCAGCGTGGAATATCa       >  1:324215/1‑140 (MQ=255)                                                                                                                               tCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCtt  <  1:711367/136‑1 (MQ=255)                                                                                                                               tCGATTGTGTGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCtt  >  2:711367/1‑136 (MQ=255)                                                                                                                                        |                                                                                                                               GGATAAACATCACCAGATCCGGGTGACCGAAATAGCGACGCTGATCGGTACGCATGACCTGATAATGTTCGGACTGTATCGGCAGCGTGTCAGCGCCGACAATACAGCCATTAGAAAAACTGCCTATCGATTGTGCGCTACCCGGCACAGGTTGGGTTATTTTTTGCCACGGCGTCGCTGCCAGGCTGGCGCTACTGGCAAGCAGAGCCAGCAGCGCAATCGCGGTTTTATTCATTTTTTACCAGCGTGGAATATCAGTCTT  >  NC_000913/2446150‑2446411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑