Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,618	T→C	E514E (GAA→GAG)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,618	0	T	C	100.0%	111.3 / NA	34	E514E (GAA→GAG)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

ACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATG  >  NC_000913/2457486‑2457753                                                                                                                                     |                                                                                                                                        aCGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCtt                                                                                                                                  <  2:492414/140‑1 (MQ=255)       tcCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCATATTTCACTGGCGAGGCATCAATGTGTTCTACCCGCTCACCTTGGGTcc                                                                                                                            >  2:332083/1‑139 (MQ=255)       tcCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCa                                                                                                                            >  1:421543/1‑140 (MQ=255)        ccATAAGCGGCTTCCGGTACAGCAACTATTTTAGTCCTGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCaa                                                                                                                           <  1:248099/140‑1 (MQ=255)              gCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCg                                                                                                                     >  2:330755/1‑140 (MQ=255)                   ttCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAg                                                                                                                >  1:133068/1‑140 (MQ=255)                   ttCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAg                                                                                                                <  2:744238/140‑1 (MQ=255)                    tCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTCCCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGc                                                                                                               <  2:312971/140‑1 (MQ=255)                              gAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCTCTCACCTAGGGTCAACATGCGTATAGCTTCATTAATg                                                                                                     >  1:368757/1‑140 (MQ=255)                              gAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATg                                                                                                     >  1:409165/1‑140 (MQ=255)                                   atTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAgg                                                                                                >  2:96302/1‑140 (MQ=255)                                   atTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAgg                                                                                                >  2:796169/1‑140 (MQ=255)                                       tAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCt                                                                                            <  1:96302/140‑1 (MQ=255)                                       tAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCt                                                                                            <  2:162771/140‑1 (MQ=255)                                            ccGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCTCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGAt                                                                                       >  2:670513/1‑140 (MQ=255)                                                            ccTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTaaaaa                                                                       <  1:504409/140‑1 (MQ=255)                                                                aTCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCgg                                                                   >  2:540330/1‑140 (MQ=255)                                                                 tCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGc                                                                  >  2:566593/1‑140 (MQ=255)                                                                 tCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGc                                                                  >  1:692637/1‑140 (MQ=255)                                                                        gTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCAGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTca                                                           <  1:82808/140‑1 (MQ=255)                                                                         ttGGATTGGGCCTACCCTAAAACCAAATTTCTCTATCGCCGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcac                                                          <  2:368757/140‑1 (MQ=255)                                                                            gATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcacgca                                                       >  2:184928/1‑140 (MQ=255)                                                                            gATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTcacgca                                                       >  2:725635/1‑140 (MQ=255)                                                                               tGGGCCTACCGGAAAACCAAATCTCACTAGCGCTGAATCAATGTGCTCAACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGa                                                    <  2:369624/140‑1 (MQ=255)                                                                                     tACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTg                                              <  1:801817/140‑1 (MQ=255)                                                                                             aaCCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTa                                      >  2:187519/1‑140 (MQ=255)                                                                                             aaCCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACCTAACAACAGGCTTTGTAACGCCCGACAATTGGCGTTTTa                                      >  2:247555/1‑140 (MQ=255)                                                                                                           aGCGCGGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGc                        <  2:692637/140‑1 (MQ=255)                                                                                                             cgcgGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCa                      >  1:501166/1‑140 (MQ=255)                                                                                                              gcgGCATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAg                     <  2:501166/140‑1 (MQ=255)                                                                                                                  cATCAATGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGtttt                 <  1:566593/140‑1 (MQ=255)                                                                                                                       aTGTGCTCTACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACtgt            <  1:670513/140‑1 (MQ=255)                                                                                                                            ctctACCCGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAg       >  2:512682/1‑140 (MQ=255)                                                                                                                                 cccGCTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTCCGGCAGTTTGACTGTGGTAGCGATg  >  1:266399/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGCTAAGATGCGATTGACGTAAAAACCGGCTTTGTCACGCACGACAATTGGCGTTTTACCCTGTTTTTTCGCCAGTTTTACTGTGGTAGCGATG  >  NC_000913/2457486‑2457753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑