Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,911	C→G	intergenic (‑79/+130)	pdeA ← / ← alaX	putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,911	0	C	G	100.0%	207.0 / NA	59	intergenic (‑79/+130)	pdeA/alaX	putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (36/23);  total (36/23)

AGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGA  >  NC_000913/2517774‑2518047                                                                                                                                          |                                                                                                                                         aGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCa                                                                                                                                        >  2:19294/1‑140 (MQ=255) aGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCa                                                                                                                                        >  1:86830/1‑140 (MQ=255)            tAGTGTGAATATCTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGTCTGTTGCCg                                                                                                                             <  2:752678/140‑1 (MQ=255)               tgtgAATATCTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGAtt                                                                                                                          >  1:588614/1‑140 (MQ=255)                        cTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCa                                                                                                                 >  2:647166/1‑140 (MQ=255)                        cTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCa                                                                                                                 >  2:85491/1‑140 (MQ=255)                        cTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCa                                                                                                                 >  1:405880/1‑140 (MQ=255)                         ttGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCaa                                                                                                                >  1:476174/1‑140 (MQ=255)                           gATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAatg                                                                                                              <  2:588614/140‑1 (MQ=255)                                tttttATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCt                                                                                                         >  1:594374/1‑140 (MQ=255)                                     aTCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCAc                                                                                                    >  2:58767/1‑140 (MQ=255)                                         ggTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAgg                                                                                                <  1:647166/140‑1 (MQ=255)                                         ggTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAgg                                                                                                <  1:558182/140‑1 (MQ=255)                                            tATGCTCCACAAACATGAGTACACTACTCTCACTGATCTGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAggcgg                                                                                             <  2:14840/140‑1 (MQ=255)                                                   cacaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAc                                                                                      >  2:136933/1‑140 (MQ=255)                                                    acaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                                                                     <  2:476174/140‑1 (MQ=255)                                                    acaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                                                                     <  2:462796/140‑1 (MQ=255)                                                    acaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                                                                     <  2:405880/140‑1 (MQ=255)                                                    acaAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                                                                     <  2:710464/140‑1 (MQ=255)                                                     caAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAccc                                                                                    >  2:102763/1‑140 (MQ=255)                                                             aGTACACCACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAg                                                                            >  2:152318/1‑140 (MQ=255)                                                              gTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                                                                           <  1:630858/140‑1 (MQ=255)                                                              gTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                                                                           <  2:696644/140‑1 (MQ=255)                                                              gTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                                                                           <  1:714719/140‑1 (MQ=255)                                                              gTACACTACTCTCACCGATCGGTGTTTTCGGGCACACTAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                                                                           <  1:235078/140‑1 (MQ=255)                                                                  actactCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                                                                     <  2:161970/126‑1 (MQ=255)                                                                  actactCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAAcc                                                                                     >  1:161970/1‑126 (MQ=255)                                                                      ctctCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                                                                           >  2:685851/1‑132 (MQ=255)                                                                      ctctCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGa                                                                           <  1:685851/132‑1 (MQ=255)                                                                      ctctCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTa                                                                   >  1:629999/1‑140 (MQ=255)                                                                      ctctCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTa                                                                   >  1:639993/1‑140 (MQ=255)                                                                       tctcACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTAc                                                                  >  1:516281/1‑140 (MQ=255)                                                                           aCTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACaaaa                                                              >  2:1070/1‑140 (MQ=255)                                                                           aCTGATCGGGGCTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACaaaa                                                              >  1:100562/1‑140 (MQ=255)                                                                            cTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAg                                                             >  1:730196/1‑140 (MQ=255)                                                                                tCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGc                                                         >  1:324276/1‑140 (MQ=255)                                                                                tCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGc                                                         >  1:723580/1‑140 (MQ=255)                                                                                tCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGc                                                         >  1:654498/1‑140 (MQ=255)                                                                                tCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCCTTCACCAGCCGGTACTCACCCGGACCTAGAATGTATTACAAAAGCGGc                                                         >  2:246515/1‑140 (MQ=255)                                                                                tCGGGCTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCAGCACTAAACCGTACGTAGACTCTATTACAAAAGCGGc                                                         >  1:296521/1‑140 (MQ=255)                                                                                     gTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCaaaaa                                                    >  2:8803/1‑140 (MQ=255)                                                                                         tCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCag                                                <  1:152318/140‑1 (MQ=255)                                                                                              cacacaAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACa                                           >  1:759772/1‑140 (MQ=255)                                                                                                cacaAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACaaa                                         >  1:745750/1‑140 (MQ=255)                                                                                                      taataaGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAAccc                                   <  1:102763/140‑1 (MQ=255)                                                                                                         taaGTTTATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCg                                <  1:85491/140‑1 (MQ=255)                                                                                                             tttATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGTAGAGACCAAAAACCCCCGCttt                            >  1:371931/1‑140 (MQ=255)                                                                                                             tttATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACaaaa                                        <  2:616352/128‑1 (MQ=255)                                                                                                             tttATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACaaaa                                        >  1:616352/1‑128 (MQ=255)                                                                                                             tttATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCttt                            >  1:536174/1‑140 (MQ=255)                                                                                                             tttATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCttt                            >  1:12414/1‑140 (MQ=255)                                                                                                               tATTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGc                          >  2:673700/1‑140 (MQ=255)                                                                                                                aTTCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCa                         >  1:494915/1‑140 (MQ=255)                                                                                                                 ttCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATCATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAg                        >  2:540139/1‑140 (MQ=255)                                                                                                                  tCGCATCGCGAAGCAAAGAGGGCGGCAGCCTGTTGGCGACTTTTCATCAAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGc                       <  2:296521/140‑1 (MQ=255)                                                                                                                  tCGCATCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGc                       <  1:8803/140‑1 (MQ=255)                                                                                                                       tCGCGAAGCAAACAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAggg                  >  2:463504/1‑140 (MQ=255)                                                                                                                              gCAAACAGGGGGGCACCCTGTTCCCCATTTTTCATCCTATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGaaa           <  1:246515/140‑1 (MQ=255)                                                                                                                                   cAGGGGGGCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTtgg      <  2:324276/140‑1 (MQ=255)                                                                                                                                       ggggCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGa  >  2:129908/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         AGCGTAAACGCTAGTGTGAATATCTTGATATTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGA  >  NC_000913/2517774‑2518047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑