Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,621,871	G→A	P225P (CCG→CCA)	purM →	phosphoribosylformylglycinamide cyclo‑ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,621,871	0	G	A	100.0%	130.4 / NA	41	P225P (CCG→CCA)	purM	phosphoribosylformylglycinamide cyclo‑ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/19);  total (22/19)

GCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGA  >  NC_000913/2621736‑2622005                                                                                                                                        |                                                                                                                                        gCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACCGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAAccc                                                                                                                                     <  1:357988/140‑1 (MQ=255)      ttGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCAtt                                                                                                                                <  2:637927/140‑1 (MQ=255)        gCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTa                                                                                                                              <  1:444898/140‑1 (MQ=255)          aCTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCGTTAGCCGTTCATCTACTGGCACCAACCCGCATTTACg                                                                                                                            >  1:292325/1‑140 (MQ=255)              ggTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGaa                                                                                                                        <  1:427169/140‑1 (MQ=255)                ttCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGt                                                                                                                      <  1:579771/140‑1 (MQ=255)                   cAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAg                                                                                                                   >  1:206964/1‑140 (MQ=255)                        gTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTg                                                                                                              >  2:593984/1‑140 (MQ=255)                        gTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGCCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTg                                                                                                              >  2:601994/1‑140 (MQ=255)                          ccACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTgta                                                                                                            <  2:675295/140‑3 (MQ=255)                          ccACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGCTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGa                                                                                                            >  1:105989/1‑140 (MQ=255)                                   aaCGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCATCCCGCATGTACGTGTAGTCAGAGCTGGAGttgattga                                                                                                   >  1:220661/1‑140 (MQ=255)                                   aaCGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGttgattga                                                                                                   >  1:502538/1‑140 (MQ=255)                                     cGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGaaa                                                                                                 <  1:601994/140‑1 (MQ=255)                                          aTTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCGCCAACCCGCATTGACGAGAAGTCAGGGCTGGAGTTGATTGAAAAGGTc                                                                                            >  2:321225/1‑140 (MQ=255)                                           ttCGCTGGTGCGCAAAATTCTTCAAGTCAGCCGTTCTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCg                                                                                           <  2:220661/140‑1 (MQ=255)                                             cGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGAt                                                                                         <  1:593984/140‑1 (MQ=255)                                                tGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGAtgtg                                                                                      >  2:6826/1‑140 (MQ=255)                                                  gTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGAGCTGGAGTTGATTGAAAAGGTCGATGTGCa                                                                                    >  1:749300/1‑140 (MQ=255)                                                   tGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCAt                                                                                   <  1:136817/140‑1 (MQ=255)                                                                       aGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACcgg                                                               >  2:693812/1‑140 (MQ=255)                                                                       aGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGAGTGAAAAGGTCGATGTGAATGCTATTGCGCACCTGACcgg                                                               >  2:324030/1‑140 (MQ=255)                                                                                 aTCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTc                                                     >  2:605169/1‑140 (MQ=255)                                                                                 aTCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTc                                                     >  1:777283/1‑140 (MQ=255)                                                                                      cAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCT‑GCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGaa                                              >  2:17500/1‑140 (MQ=255)                                                                                                      aTGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTa                               <  2:502538/140‑1 (MQ=255)                                                                                                      aTGGTAAGCCATTAGCCGATCAGCTGCTGGCCCCATCCCTCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTa                               <  2:244349/140‑1 (MQ=255)                                                                                                       tGGTAAGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTAt                              <  2:519752/140‑1 (MQ=255)                                                                                                         gTAAGCCATTAGCCGTTCATCTACTTGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTg                            <  1:324030/140‑1 (MQ=255)                                                                                                         gTAAGCCATTAGCCGATCATCTGCTGGCACCAACCGGCAATTACGTGAGGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTg                            <  2:326529/140‑1 (MQ=255)                                                                                                           aaGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGcc                          <  2:474917/140‑1 (MQ=255)                                                                                                            aGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCTGGGGAAAACATTCCGCGCGTATTGcc                          >  2:669476/1‑140 (MQ=255)                                                                                                            aGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCa                         >  2:173675/1‑140 (MQ=255)                                                                                                            aGCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCa                         >  1:89592/1‑140 (MQ=255)                                                                                                             gCCATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGAGGCGGCTACT‑GTGAGAACATTACGCGCGTATTGCCAg                        >  1:244349/1‑140 (MQ=255)                                                                                                               cATTAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGat                      <  1:605169/140‑1 (MQ=255)                                                                                                                  tAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGataat                   >  1:156561/1‑140 (MQ=255)                                                                                                                  tAGCCGATCATCTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGataat                   >  2:99555/1‑140 (MQ=255)                                                                                                                        atcatcTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAg             >  2:480439/1‑140 (MQ=255)                                                                                                                         tcatcTGCTGGCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAgg            <  2:105989/140‑1 (MQ=255)                                                                                                                                   gCACCAACCCGCATTTAGGTGAAGTCAGTGCTGGAATTGTTTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGtgattga  <  2:292325/140‑1 (MQ=255)                                                                                                                                   gCACCAACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGtgattga  >  1:152316/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GCTGATTGCACTCGGTTCCAGCGGTCCACACTCGAACGGCTATTCGCTGGTGCGCAAAATTCTTGAAGTCAGCGGTTGTGATCCGCAAACCACCGAACTTGATGGTAAGCCATTAGCCGATCATCTGCTGGCACCGACCCGCATTTACGTGAAGTCAGTGCTGGAGTTGATTGAAAAGGTCGATGTGCATGCCATTGCGCACCTGACCGGCGGCGGCTTCT‑GGGAAAACATTCCGCGCGTATTGCCAGATAATACTCAGGCAGTGATTGA  >  NC_000913/2621736‑2622005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑