Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,372	G→A	intergenic (‑241/+211)	iscR ← / ← trmJ	DNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,372	0	G	A	100.0%	105.3 / NA	36	intergenic (‑241/+211)	iscR/trmJ	DNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/16);  total (20/16)

GACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTG  >  NC_000913/2662246‑2662505                                                                                                                               |                                                                                                                                      gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGGCCTGGGGTGCTGGACAGTGTAGGCCCGATAGGACACATTACGCGTCGc                                                                                                                          >  2:217566/1‑140 (MQ=37) gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGc                                                                                                                          >  1:115521/1‑140 (MQ=255)  aCTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCa                                                                                                                         >  1:8796/1‑140 (MQ=255)    tGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATc                                                                                                                       >  1:478214/1‑140 (MQ=255)                 tATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGt                                                                                                          >  2:63770/1‑140 (MQ=255)                     tAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                      <  2:470567/140‑1 (MQ=255)                           tCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCTTCAGGCAACGGCTGTCGGATgcggc                                                                                                >  1:369988/1‑140 (MQ=255)                             aGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt                                                                                              >  1:153169/1‑140 (MQ=255)                             aGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt                                                                                              >  1:566051/1‑140 (MQ=255)                              gTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTg                                                                                             <  2:115521/140‑1 (MQ=255)                               tGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGa                                                                                            <  1:701780/140‑1 (MQ=255)                                  cATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACg                                                                                         <  1:63770/140‑1 (MQ=255)                                   aTTTTTTTTTCGTGGCCATTGTTCCTGTTGCAGTACGCGTCGTGAACGGCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGc                                                                                        <  2:397745/140‑1 (MQ=17)                                               tGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCt                                                                            >  1:525590/1‑140 (MQ=255)                                                ggCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                                           <  2:153169/140‑1 (MQ=255)                                                    tttGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGt                                                                       <  2:8796/140‑1 (MQ=255)                                                       gTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCt                                                                    <  2:478214/140‑1 (MQ=255)                                                          ccTGTTGTCGGATGCTGCGTGAACGACATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                                                 <  1:337949/140‑1 (MQ=255)                                                          ccTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                                                 <  1:749901/140‑1 (MQ=255)                                                          ccTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                                                 <  2:566051/140‑1 (MQ=255)                                                          ccTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                                                 <  2:520333/140‑1 (MQ=255)                                                              ttgtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGGGAACGCCTTAGCCGAAATACGGTTCTGTTCACt                                                             >  2:368308/1‑140 (MQ=14)                                                               tgtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTg                                                            >  1:599162/1‑140 (MQ=255)                                                                     aTGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGcc                                                      <  1:628697/140‑1 (MQ=255)                                                                      tGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCCTCAGGCAACGGCTGTCGGAGGCTGCGTGAACGTCTTCTCCGTCCTGCGGTCCTGTTCACTGTAGGccg                                                     >  2:291898/1‑139 (MQ=14)                                                                         ggcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGcc                                                      >  1:509246/1‑136 (MQ=255)                                                                         ggcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGcc                                                      <  2:509246/136‑1 (MQ=255)                                                                                        tCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcgcg                                   <  2:525590/140‑1 (MQ=255)                                                                                                              tGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCg             >  1:153657/1‑140 (MQ=14)                                                                                                              tGTAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCg             >  1:431047/1‑140 (MQ=14)                                                                                                                tAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGa           >  2:511917/1‑140 (MQ=18)                                                                                                                tAGGCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGa           >  1:642805/1‑140 (MQ=18)                                                                                                                   gCCTGATAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATgc        >  1:197899/1‑140 (MQ=25)                                                                                                                        aTAAGACACATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt   >  1:438372/1‑140 (MQ=255)                                                                                                                        aTAAGACACATTACGCGTCACATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGACAAGACGCATTACGCGTCGCATGAGTCAACGGATGTCGGATgcggcgg   >  1:380410/1‑139 (MQ=14)                                                                                                                         tAAGACACATTACGCGTCGCATTAGGCAACGGCTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTg  <  2:342572/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTG  >  NC_000913/2662246‑2662505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑