Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,342	G→A	I734I (ATC→ATT)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,342	0	G	A	100.0%	117.6 / NA	37	I734I (ATC→ATT)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

ACATGGAGTCTTTACCCACCGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAG  >  NC_000913/2693206‑2693474                                                                                                                                         |                                                                                                                                     aCATGGAGTCTTTACCCACCGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCATCACCAGGATGGACTGCCGCCGCCAGCCAGTTGGCGGAAAGTTTAATg                                                                                                                                   >  2:251950/1‑140 (MQ=255)      gAGTCTTTACCCACCGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGttt                                                                                                                              >  1:171121/1‑140 (MQ=255)            ttACCCACCGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCACCATCCATTTGTCGAAAAGTTAAATGCGTTTGATATc                                                                                                                        >  1:267226/1‑140 (MQ=255)                ccACCGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCAGCCGCCGCAATCGAGTAGGCGGAAACTTTAATGCGTTTGATATCGCCa                                                                                                                    >  2:289522/1‑140 (MQ=255)                   ccGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCCGTTGGCGAAAAGTTAAATGCGATTGATATCGCCAAtt                                                                                                                 >  1:300313/1‑140 (MQ=255)                     gggATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGGTGGCGGAAAGTGTAATGCGTTGGATATCGCCAATTtg                                                                                                               >  1:229992/1‑140 (MQ=255)                      ggATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTtgt                                                                                                              >  2:462846/1‑140 (MQ=255)                        aTCGTCAGGCCCAGCTCCGGACCAACCTCCTCGCCCATGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTgtgt                                                                                                            <  1:251950/140‑1 (MQ=255)                             cAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTgcgg                                                                                                       >  1:693407/1‑140 (MQ=255)                               ggCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTgcggcg                                                                                                     <  2:171121/140‑1 (MQ=255)                                gCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGACTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGa                                                                                                    <  2:487395/140‑1 (MQ=255)                                                     ttCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTATGATATCGGCAATTAGAGTTGCGGCGATGTACGTTAACGTTTCaccga                                                                               >  2:400238/1‑140 (MQ=255)                                                         cccACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGGGATGTTGGTTAACGCTTCACCGACCGc                                                                           <  2:756967/140‑1 (MQ=255)                                                                  ttAACGGCTTCATACAGGCCCGGATCTTCGCCAGGGTTGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGc                                                                  <  1:289522/140‑1 (MQ=255)                                                                          ttCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggcgg                                                          >  1:614624/1‑140 (MQ=255)                                                                            cATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGgcggcg                                                        >  1:549453/1‑140 (MQ=255)                                                                                   gCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCca                                                 >  1:166162/1‑140 (MQ=255)                                                                                   gCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCca                                                 >  1:649458/1‑140 (MQ=255)                                                                                   gCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCca                                                 >  1:699428/1‑140 (MQ=255)                                                                                        cATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                                            >  2:722797/1‑140 (MQ=255)                                                                                        cATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCagcagc                                            >  1:24627/1‑140 (MQ=255)                                                                                                    ggTGGCCTGCCGCCGCCATCCACTTGGCGGACAGTTTAACGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCa                                <  2:300313/140‑1 (MQ=255)                                                                                                     gTGGCCTGCCGCCGCTATCCAGTTGGCGGTAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCAc                               <  1:400238/140‑1 (MQ=255)                                                                                                     gTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCAc                               >  1:425797/1‑140 (MQ=255)                                                                                                        gCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCt                            <  2:693407/140‑1 (MQ=255)                                                                                                             ccgccgccATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCa                       >  1:714639/1‑140 (MQ=255)                                                                                                              cgccgccATCCAGTTGGGGGAAAGTTTAATGAGTTAGATCTCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCaa                      <  2:267226/140‑1 (MQ=255)                                                                                                                    cATCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCa                <  1:722797/140‑1 (MQ=255)                                                                                                                     aTCCAGTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCAt               <  1:462846/140‑1 (MQ=255)                                                                                                                          gTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCtt          <  2:649458/140‑1 (MQ=255)                                                                                                                          gTTGGCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCtt          <  2:650658/140‑1 (MQ=255)                                                                                                                             ggCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCAc       >  2:137553/1‑140 (MQ=255)                                                                                                                             ggCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCAc       <  2:714639/140‑1 (MQ=255)                                                                                                                             ggCGGAAAGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCAc       >  2:75203/1‑140 (MQ=255)                                                                                                                               cGGAAAGTTTAATGCGTCTGATCTCGCCAATCTGTCTTGCGGCGATGTGGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCg     <  2:229992/140‑1 (MQ=255)                                                                                                                                  aaaGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgtag  <  2:699428/140‑1 (MQ=255)                                                                                                                                  aaaGTTTAATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgtag  <  2:166162/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     ACATGGAGTCTTTACCCACCGGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAG  >  NC_000913/2693206‑2693474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑