Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,134	A→G	H122R (CAC→CGC)	mprA →	DNA‑binding transcriptional repressor MprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,134	0	A	G	100.0%	114.2 / NA	35	H122R (CAC→CGC)	mprA	DNA‑binding transcriptional repressor MprA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

GTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGA  >  NC_000913/2811005‑2811262                                                                                                                                  |                                                                                                                                 gtgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTg                                                                                                                        <  1:236014/140‑1 (MQ=255)  tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCGTCTGCAATTAACAGAAAAAGGTCGCGAGTTTTTgc                                                                                                                       >  2:363893/1‑140 (MQ=255)  tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgc                                                                                                                       >  2:353355/1‑140 (MQ=255)   gCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcg                                                                                                                      <  1:50191/140‑1 (MQ=255)    ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                     <  2:492070/140‑1 (MQ=255)            tcatcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGtttt                                                                                                             >  1:517500/1‑140 (MQ=255)              atcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                           <  1:353355/140‑1 (MQ=255)                 ccGTACCAACGCGACTCGTATTCCCCATGAACTGGAAGAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                        <  1:256414/140‑1 (MQ=255)                 ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                        >  2:449272/1‑140 (MQ=255)                 ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                        <  1:442466/140‑1 (MQ=255)                   gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                      <  2:691246/140‑1 (MQ=255)                   gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                      <  2:420114/140‑1 (MQ=255)                   gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                      <  2:517500/140‑1 (MQ=255)                     aCCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAATGGTAGCGAGTTTATGCGAGAGGTTTTACCACCgca                                                                                                    >  2:256414/1‑140 (MQ=255)                          cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                               >  2:1009/1‑140 (MQ=255)                           gcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATaa                                                                                              <  1:1009/140‑1 (MQ=255)                              acgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActg                                                                                           >  2:562473/1‑140 (MQ=255)                                gcgTATTGCCGATGATCTGGAAAAACGCGTTTGGATCTAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                         <  1:358652/140‑1 (MQ=255)                                gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                         <  1:449272/140‑1 (MQ=255)                                     ttGCCGATGAACTGGAACAACGTGGTTGGATTGTAGGTCGTGAAAGCGCTAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                    <  2:373510/140‑1 (MQ=255)                                            tGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGATTTTGCGCAAGGCTTTACCACCGCAGCATACCTGCCTGCATCAActct                                                                             >  2:308310/1‑140 (MQ=255)                                                cTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTAGCGCGAGGTTTTACCAACGCAGCATAACTGGCTGCATCAACTCTGGTc                                                                         >  2:211990/1‑140 (MQ=255)                                                        aCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCATCGCAGCATAACTGCCTACATCGACTCTGGTCCGCGCTCa                                                                 >  2:322865/1‑140 (MQ=255)                                                        aCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCa                                                                 >  2:150964/1‑140 (MQ=255)                                                         cgcgGTTGGATCGAACTGCGTGAAAGCGATATCCATCGCTGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAg                                                                <  2:371333/140‑1 (MQ=255)                                                           cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTCCAACAACTCAGGTCCGCGCTCAGca                                                              >  1:371333/1‑140 (MQ=255)                                                            ggTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGcac                                                             <  1:562473/140‑1 (MQ=255)                                                                                     aTAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCaa                                    >  2:583362/1‑140 (MQ=255)                                                                                           aTCGCCGCTGCCTGCATCTGCACTTCACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCAcc                              <  1:308310/140‑1 (MQ=255)                                                                                           aTCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTC‑GGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCAccc                             <  1:755798/140‑1 (MQ=255)                                                                                                         cATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCtctc                <  2:14434/140‑1 (MQ=255)                                                                                                          aTCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc               >  2:821763/1‑140 (MQ=255)                                                                                                               cAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGtct          >  2:433650/1‑140 (MQ=255)                                                                                                                  ttAACGGAAAAAGGTCGCGAGTTTTCGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGa       <  1:363893/140‑1 (MQ=255)                                                                                                                       ggAAAAAGGTCGCGAGTTTTTGCGCCAGGTTTTACCATCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa  <  1:354111/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 GTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGA  >  NC_000913/2811005‑2811262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑