Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,818,463	A→G	intergenic (‑189/+10)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,463	0	A	G	100.0%	114.1 / NA	36	intergenic (‑189/+10)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

ATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGAC  >  NC_000913/2818326‑2818595                                                                                                                                          |                                                                                                                                     aTCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTTCCGCCACTTCTGCCATACCTTGATCGCTGAAAGTGCg                                                                                                                                    >  1:234340/1‑140 (MQ=17) aTCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCg                                                                                                                                    >  1:668833/1‑140 (MQ=255)   cAAGTAAGAGATGGTGCATCCGGGAGGATCACTCGCCCTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTa                                                                                                                                  <  1:302587/140‑1 (MQ=255)             aTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTg                                                                                                                        <  2:668833/140‑1 (MQ=255)               ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCa                                                                                                                      >  2:48729/1‑140 (MQ=255)                gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCCGCGGGTATTACCGCCACCTCTTCCACACCTTCATCGCTGTAAGTGCGTAGAAAATGGTGCAt                                                                                                                     >  2:166283/1‑140 (MQ=18)                gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGCTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGACACCTCTTACATACCTACATCGCTGAAAGACCGTAGAATATGGTGCAt                                                                                                                     >  2:323624/1‑140 (MQ=18)                     tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATTGTGCATCCggg                                                                                                                >  1:74734/1‑140 (MQ=255)                     tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCggg                                                                                                                >  1:570612/1‑140 (MQ=255)                     tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCggg                                                                                                                >  1:536487/1‑140 (MQ=255)                      ccGGGAGGATTACTCTCCTTGCGGCTCGCTCTTCGGGGCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGgga                                                                                                               <  1:323624/140‑1 (MQ=255)                        gggAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGggagg                                                                                                             <  1:166283/140‑1 (MQ=255)                            gacagaCTCGCCTTGAGGCTCGTCCATCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTc                                                                                                         <  1:388840/135‑1 (MQ=18)                                          gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGTATATGGTGCATCCGAGAGGATTCGAACCTCCGACCGc                                                                                           >  1:267525/1‑140 (MQ=255)                                          gCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCTAACCTCCGACCGc                                                                                           >  1:55356/1‑140 (MQ=255)                                           cGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                          <  1:99799/140‑1 (MQ=255)                                               tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCAAACCTCCGACCGCTCGGt                                                                                      <  1:409996/140‑1 (MQ=255)                                                    cTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                 <  1:706426/140‑1 (MQ=255)                                                         ggCCGTTGCTAAAGCCACGTTACCCTCCCGGGTGCCTTCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                            <  1:249825/140‑1 (MQ=18)                                                         ggCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                            <  1:48729/140‑1 (MQ=255)                                                            cGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                         <  2:536487/140‑1 (MQ=255)                                                                   aaaGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCTAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                  <  2:55356/140‑1 (MQ=255)                                                                   aaaGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATc                                                                  <  2:570612/140‑1 (MQ=255)                                                                        aaCGTTATCCTCCCTGGTGCCTACGGTTATTTCCGCCACTTCTTCCATCCCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                             <  2:267525/140‑1 (MQ=17)                                                                          cGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                           >  1:637574/1‑140 (MQ=255)                                                                               tcctccCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                      >  2:92609/1‑140 (MQ=255)                                                                                cctccCTGGTACCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGAAGCGGAGTACTCTGTCCAGCTGAGCTACg                                                     >  1:264254/1‑140 (MQ=18)                                                                                     cTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTGCGTAGCCAAGTTCTATATCCAGCTGAGCTACGGATGc                                                >  1:239767/1‑140 (MQ=17)                                                                                         tGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAAt                                            >  2:565199/1‑140 (MQ=255)                                                                                               cGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGt                                      <  2:637574/140‑1 (MQ=255)                                                                                                  ttattaCCGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAGTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAg                                   >  2:618221/1‑140 (MQ=255)                                                                                                         cGCCACCTCTTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGa                            >  2:71146/1‑140 (MQ=255)                                                                                                                 ctTCCATACCTTCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGGTACGGATACAAATGTCGGTGAGGCGGGGATTCGAAcc                    >  1:222202/1‑140 (MQ=255)                                                                                                                            tCATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGc         >  1:192114/1‑140 (MQ=255)                                                                                                                             cATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTTGAACCCCGGATGCAGCt        >  1:696029/1‑140 (MQ=255)                                                                                                                             cATCGCTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGGTTGAACCCCGGGTGGAggt        >  2:515215/1‑138 (MQ=18)                                                                                                                                  cTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGa   <  1:695044/140‑1 (MQ=255)                                                                                                                                  cTGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGa   <  1:565199/140‑1 (MQ=255)                                                                                                                                   tGAAAGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGAc  <  2:74734/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     ATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAAAGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGAC  >  NC_000913/2818326‑2818595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑