Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,424	T→C	G11G (GGT→GGC)	srlE →	sorbitol‑specific PTS enzyme IIBC1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,424	0	T	C	100.0%	84.8 / NA	28	G11G (GGT→GGC)	srlE	sorbitol‑specific PTS enzyme IIBC1 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/13);  total (15/13)

CTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGA  >  NC_000913/2826296‑2826550                                                                                                                                 |                                                                                                                               ctcccGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGggcgg                                                                                                                     <  2:91066/137‑1 (MQ=255)      gcgGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGc                                                                                                                <  1:746856/140‑1 (MQ=255)       cgGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGGTCGAAAAAGTAACGGGCGGATGGGGCGGCGCGCt                                                                                                               >  1:511534/1‑140 (MQ=255)       cgGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCt                                                                                                               >  1:421866/1‑140 (MQ=255)             ggTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCt                                                                                                         <  2:582504/140‑1 (MQ=255)                  ccGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAACAAGTAACGGGCGGCTGTGGCGGCCCGCTTGAGCTGAAAg                                                                                                    >  2:343008/1‑140 (MQ=255)                   cGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGc                                                                                                   >  2:444220/1‑140 (MQ=255)                                 ccATTTTTGAGAAAAAGGTGGGAATTCAACTTGAACAAACAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaa                                                                                     <  2:313219/140‑1 (MQ=255)                                    tttttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCACTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                                  <  1:47499/140‑1 (MQ=255)                                      tttGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATc                                                                                <  1:674642/140‑1 (MQ=255)                                         gagaAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTc                                                                             <  1:115901/140‑1 (MQ=255)                                           aaaaaaGATGGGCATTCAACTTGAACAAAAAGTTCCCCTGGCAGGAGCAACATCATGACGCATATTCGGACCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAATCGTCta                                                                           <  2:546369/139‑1 (MQ=255)                                                   ggggAATTCAACTTGAACAAAAAGTTCCCCTGGCAGGAGCAACATCATGACGCATATTCGGATCAAAAAAGGAACGGGCGGCGGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCAccg                                                                   <  2:421866/139‑1 (MQ=255)                                                          tCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAATATCATGACGCATATTCGGATCGAAAAAGGAAGGGGCGGCTGGGGCGG‑CCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAcc                                                           >  1:555373/1‑140 (MQ=255)                                                          tCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAc                                                            >  2:791639/1‑140 (MQ=255)                                                                        aaGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGAttg                                              >  2:668728/1‑140 (MQ=255)                                                                            cccccgggCAGGAGCAACATCATGACGCATACTCGCATAGAAAAAGGAGGGGGCGGGGGGGGGGCCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGa                                          <  2:305018/134‑1 (MQ=255)                                                                                   gCAGGAGAAACATCATGACGAATATACGGATCGAAAATGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTAAAAGCCACGCGGGAAAAAAAAATCGTCTATACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTg                                   <  1:343008/140‑1 (MQ=255)                                                                                        aGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcaca                              >  2:519071/1‑140 (MQ=255)                                                                                             catcatGACGCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATGTCACCGCCGGTATCCGGCCTGCGATAGTTGGCAAACTGGCACAGCTTa                         >  2:358048/1‑140 (MQ=255)                                                                                               tcatGACGCATATTCGGATCAAAAAAGGAACGGGCGGCGGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTAct                       <  1:446036/140‑1 (MQ=255)                                                                                                      gCATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGATGATAAGCTGGCAGAGCTTACtggctggc                >  1:245924/1‑140 (MQ=255)                                                                                                       cATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCa               >  1:411901/1‑140 (MQ=255)                                                                                                       cATATTCGGATCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTACGATTGTAGACAAAGTGGCACTGCTTACTGGCTGGCa               >  1:362806/1‑140 (MQ=255)                                                                                                                agcaaaaagGGAAGGGGGGGCGGGGGCGGCCCCCTTGAGCGGAAAGCCAGGCAGGAAAAAAAAATCGTCAATACCTCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa      <  2:61427/136‑1 (MQ=255)                                                                                                                aTCGAAAAAGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa      <  1:791639/140‑1 (MQ=255)                                                                                                                aTCGAAAAAGGAACGGGCGGCGGGGGCGGCCCGCTTGAGCTGAAAGCCACGCGGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa      <  1:780438/140‑1 (MQ=255)                                                                                                                    aaaaaGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGa  >  2:107302/1‑140 (MQ=255)                                                                                                                    aaaaaGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGa  >  1:754149/1‑140 (MQ=255)                                                                                                                    aaaaaGGAACGGGCGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGa  >  1:52956/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                               CTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGA  >  NC_000913/2826296‑2826550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑