Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,828,221	T→G	V162G (GTC→GGC)	srlD →	sorbitol‑6‑phosphate 2‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,828,221	0	T	G	100.0%	154.1 / NA	44	V162G (GTC→GGC)	srlD	sorbitol‑6‑phosphate 2‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/25);  total (19/25)

TCCTGTGTGCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAA  >  NC_000913/2828086‑2828356                                                                                                                                        |                                                                                                                                        tCCTGTGTGCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCggg                                                                                                                                     <  2:705149/140‑1 (MQ=255)      tgtgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGAc                                                                                                                                >  2:98760/1‑140 (MQ=255)        tgCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTc                                                                                                                              >  2:572505/1‑140 (MQ=255)         gcgcGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCa                                                                                                                             >  1:502625/1‑140 (MQ=255)          cgcgTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCaa                                                                                                                            >  1:537169/1‑140 (MQ=255)                  ttttCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGgc                                                                                                                    >  1:78708/1‑140 (MQ=255)                     tCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGACTGACTCAATCACTGGCGCt                                                                                                                 >  1:708793/1‑140 (MQ=255)                        cgTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGa                                                                                                              <  1:490615/140‑1 (MQ=255)                             gatgatCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTgg                                                                                                         <  2:812461/140‑1 (MQ=255)                                    cgcgACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTa                                                                                                  <  2:561616/140‑1 (MQ=255)                                    cgcgACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTa                                                                                                  <  2:537169/140‑1 (MQ=255)                                       gACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACgg                                                                                               >  2:27365/1‑140 (MQ=255)                                         cGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCa                                                                                             <  2:727677/140‑1 (MQ=255)                                            gATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTa                                                                                          <  1:572505/140‑1 (MQ=255)                                              ttCAGGGGCGCATTATTAAGATAAACTCGAACTCCCGCATAGTGGGCATCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACg                                                                                        <  2:314073/140‑1 (MQ=255)                                               tCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACgg                                                                                       <  2:9301/140‑1 (MQ=255)                                               tCAGGGGCGCATTATCCAGATCGACCCGAAATCCGGCGAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACgg                                                                                       <  1:362525/140‑1 (MQ=255)                                                   gggCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATAACTGGCGCTGGATCTGGCGGATGACGGCATTACGGTGCa                                                                                   >  2:362525/1‑140 (MQ=255)                                                        cATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTAAATCACTGTCGCTGGATCTGGCGGAATACGCCATTACGGTGCATTCAc                                                                              >  2:354480/1‑140 (MQ=255)                                                          ttattCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTg                                                                            <  1:824430/140‑1 (MQ=255)                                                                   tCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGt                                                                   <  2:78708/140‑1 (MQ=255)                                                                     aaCTCGAAATCCGGCAAAGTGGACAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTaa                                                                 >  1:156802/1‑140 (MQ=255)                                                                      aCTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCAATGATGCTCGGTAAc                                                                <  2:502625/140‑1 (MQ=255)                                                                       cTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAAcc                                                               >  2:688670/1‑140 (MQ=255)                                                                            aaTCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACctgctg                                                          >  1:616636/1‑140 (MQ=255)                                                                                     aaGTGGGCAGCAAACTCAACTCTGGATACAGCGCAGCAAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCg                                                 <  1:268614/140‑1 (MQ=255)                                                                                     aaGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCg                                                 <  2:652580/140‑1 (MQ=255)                                                                                     aaGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCg                                                 <  2:616636/140‑1 (MQ=255)                                                                                         gggCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGt                                             <  1:49939/140‑1 (MQ=255)                                                                                                aaaCACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTc                                      <  2:647876/140‑1 (MQ=255)                                                                                                 aaCACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCa                                     >  2:9732/1‑140 (MQ=255)                                                                                                  acacAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCAc                                    >  1:704652/1‑140 (MQ=255)                                                                                                      aaCTCTGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGtt                                <  2:156802/140‑1 (MQ=255)                                                                                                        ctctGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGc                              <  1:768502/140‑1 (MQ=255)                                                                                                           tGGCTACAGCGCAGCGAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCcac                           >  2:740926/1‑140 (MQ=255)                                                                                                                 cagcgcagcgAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATAcg                     <  1:9732/140‑1 (MQ=255)                                                                                                                  agcgcagggAAATTTGGTGTCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATa                       >  2:472765/1‑137 (MQ=255)                                                                                                                  agcgcagcgAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATa                       <  1:472765/137‑1 (MQ=255)                                                                                                                  agcgcagcgAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATAcgc                    >  2:455181/1‑140 (MQ=255)                                                                                                                       agcgAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCCCCGATGTTCCAGTCACTGTTGTCACAATACGCGACCa               >  1:343261/1‑140 (MQ=255)                                                                                                                         cgAAATTTGGTGGCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAg             <  1:455181/140‑1 (MQ=255)                                                                                                                            aaTTTGGTGGCGGCGGGCTGACCCAATCACTTGCGATGGTTCTGGCGGTGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTg          <  2:262640/140‑1 (MQ=255)                                                                                                                            aaTTTGGTGGCGGCGGACTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTg          <  2:708793/140‑1 (MQ=255)                                                                                                                                 ggtggCGGCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTAt     >  2:189931/1‑140 (MQ=255)                                                                                                                                    ggcggcGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCaa  >  1:196423/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TCCTGTGTGCGCGTGAATTTTCGCGTTTGATGATCCGCGACGGGATTCAGGGGCGCATTATTCAGATCAACTCGAAATCCGGCAAAGTGGGCAGCAAACACAACTCTGGCTACAGCGCAGCGAAATTTGGTGGCGTCGGGCTGACTCAATCACTGGCGCTGGATCTGGCGGAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAA  >  NC_000913/2828086‑2828356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑