Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,325	T→C	D267G (GAT→GGT)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,325	0	T	C	100.0%	145.9 / NA	42	D267G (GAT→GGT)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/24);  total (18/24)

GTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCT  >  NC_000913/2983195‑2983460                                                                                                                                   |                                                                                                                                        gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCt                                                                                                                                >  1:162942/1‑140 (MQ=255)       tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGtttt                                                                                                                          >  1:124476/1‑140 (MQ=255)            caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCg                                                                                                                     >  2:24742/1‑140 (MQ=255)               ccAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGAcc                                                                                                                  <  2:147129/140‑1 (MQ=255)                 aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCAt                                                                                                                >  2:753913/1‑140 (MQ=255)                    aacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGcc                                                                                                             <  2:461011/139‑1 (MQ=255)                     acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGccc                                                                                                            >  2:606667/1‑140 (MQ=255)                       gacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCa                                                                                                          >  1:615902/1‑140 (MQ=255)                           gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                                                      <  1:24742/140‑1 (MQ=255)                           gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                                                      <  1:46041/140‑1 (MQ=255)                               cTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAgg                                                                                                  >  2:531569/1‑140 (MQ=255)                                 ttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                                                                <  2:162942/140‑1 (MQ=255)                                                   gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                                                              <  2:124476/140‑1 (MQ=255)                                                   gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                                                              <  2:765101/140‑1 (MQ=255)                                                   gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                                                              <  2:555697/140‑1 (MQ=255)                                                   gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                                                              <  1:531569/140‑1 (MQ=255)                                                       ttAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCgg                                                                          >  2:762953/1‑140 (MQ=255)                                                         aaaaTCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                                                              <  1:611644/134‑1 (MQ=255)                                                         aaaaTCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                                                              >  2:611644/1‑134 (MQ=255)                                                                 aaCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                                                                >  1:198523/1‑140 (MQ=255)                                                                        ccTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc                                                         >  2:122648/1‑140 (MQ=255)                                                                           tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCa                                                             <  2:752989/133‑1 (MQ=255)                                                                           tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCa                                                             >  1:752989/1‑133 (MQ=255)                                                                           tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                                                      >  1:597509/1‑140 (MQ=255)                                                                                       cccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGcc                                          <  1:753913/140‑1 (MQ=255)                                                                                        ccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCCCCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCt                                         <  1:510057/140‑1 (MQ=255)                                                                                        ccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCt                                         >  2:185859/1‑140 (MQ=255)                                                                                          acaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGc                                       <  1:185859/140‑1 (MQ=255)                                                                                           caACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCt                                      <  2:615902/140‑1 (MQ=255)                                                                                           caACTAGCGCAAATCTTAAACGACCACATGGGCCAAATCCCCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCt                                      <  2:303954/140‑1 (MQ=255)                                                                                             aCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCg                                    <  1:122648/140‑1 (MQ=255)                                                                                             aCTAGCGAAAATTTTAAACGGCCACATGGTCCATATCACAAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCg                                    <  2:198523/140‑1 (MQ=255)                                                                                                aGCGCAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTa                                 <  1:762627/140‑1 (MQ=255)                                                                                                  cgcAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATg                               >  2:808424/1‑140 (MQ=255)                                                                                                  cgcAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATg                               <  1:199222/140‑1 (MQ=255)                                                                                                  cgcAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATg                               <  2:29442/140‑1 (MQ=255)                                                                                                  cgcAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATg                               <  1:606667/140‑1 (MQ=255)                                                                                                  cgcAAATCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATg                               <  1:762953/140‑1 (MQ=255)                                                                                                  cccAAATCTTAAACGGCCACATGGTCCATACCCCCAAAGTCCTGGTTTTCCCCGACCATGCCCCAGATAAAGGTATAAACTTTGGTCCCGACACAGAAACGGCTCCACCAGCTCGGGGATATCACCGCCTGCTCGTTATg                               <  2:180686/138‑1 (MQ=255)                                                                                                     aaaTCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGAACGAACAGGTCGGGGAGGTCACCGCCTGCTCGTTATGCAt                            >  2:305874/1‑140 (MQ=255)                                                                                                      aaTCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGTCCAGCTCTGGGGGATCACCGCCTGCTCGTTATGCATc                           >  1:303954/1‑140 (MQ=255)                                                                                                      aaTCTTTAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATc                           >  1:72455/1‑140 (MQ=255)                                                                                                      aaTCTTTAACGGCCAAATGGTCAATGTCACCAAAGACATGGTTTTCGCCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATc                           <  1:321360/140‑1 (MQ=255)                                                                                                           ttAACGGCCACATGGTCCATATCACCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAat                      <  2:597509/140‑1 (MQ=255)                                                                                                                               atcaccAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCt  <  2:72455/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        GTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCTCGTTATGCATCACAATATGACGCGTTTCTTGCGGCT  >  NC_000913/2983195‑2983460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑