Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,048,383	A→G	V220A (GTA→GCA)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,048,383	0	A	G	100.0%	76.0 / NA	23	V220A (GTA→GCA)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

TATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCA  >  NC_000913/3048249‑3048518                                                                                                                                       |                                                                                                                                        tatCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCctgcctgc                                                                                                                                    >  2:444585/1‑140 (MQ=255)             gTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACg                                                                                                                        <  2:120085/140‑1 (MQ=255)                  cccGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGaa                                                                                                                   >  2:78340/1‑140 (MQ=255)                      gcgcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACg                                                                                                               >  1:416766/1‑140 (MQ=255)                          aGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCt                                                                                                           <  1:631292/140‑1 (MQ=255)                                cAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACTATTTTGCGCGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGt                                                                                                     <  2:246989/140‑1 (MQ=255)                                  gCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCg                                                                                                   <  2:36544/140‑1 (MQ=255)                                      cAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCa                                                                                               >  1:36285/1‑140 (MQ=255)                                                  atCGGCGGCAACGCTGAACACAATTTTGCGTGATTTCAGTTCGCTAATTAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGt                                                                                   <  2:338481/140‑1 (MQ=255)                                                      gcggcAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAGCAGCACGCCGAAGACGCCCTGATGGCCGAGTACTTTTTGCGCGTCATc                                                                               >  2:278519/1‑140 (MQ=255)                                                            aCGCTGACCTCAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAAt                                                                         <  2:473103/140‑1 (MQ=255)                                                                          tttGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCaa                                                           <  1:78340/140‑1 (MQ=255)                                                                                 gATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTGGCGCGTCATCGACAATCACTTGAAAACCAAAGGTTTc                                                    >  1:263174/1‑140 (MQ=255)                                                                                   tttCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGCGCACTTTTCGCGAGTCTTCGACAATCACTTCAAAACCAAAGGTTTCgg                                                  >  2:281621/1‑140 (MQ=255)                                                                                           cGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTa                                          <  2:36285/140‑1 (MQ=255)                                                                                           cGCTAATAAGCGCAGCGTAGTCGTGCATTCCACCGGTCGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTa                                          <  1:401370/140‑1 (MQ=255)                                                                                                ataaGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGAc                                     <  1:590002/140‑1 (MQ=255)                                                                                                ataaGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGAc                                     <  1:525811/140‑1 (MQ=255)                                                                                                            tAGTCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTGTCGCCAAGAGTACCGACCACATCCAGCGt                         >  1:362991/1‑140 (MQ=255)                                                                                                               tCGTGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTg                      >  2:183912/1‑140 (MQ=255)                                                                                                               tCGTGAATTTCACCGATAGTGCCTGTCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTg                      <  1:281621/140‑1 (MQ=255)                                                                                                                  tGAATTTCACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCgg                   >  2:625585/1‑140 (MQ=255)                                                                                                                         cACCGGTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGcaca            >  2:831438/1‑140 (MQ=255)                                                                                                                              gTAGTGCCTGCCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGa       <  1:444585/140‑1 (MQ=255)                                                                                                                                   gcctgcctGCTGTAAAAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCa  <  1:278519/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCAAAGGTTTCGGCACGAGTACGGACCACATCCAGCGTTTGCGGATGCACATCGGAAGCCA  >  NC_000913/3048249‑3048518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑