Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,588	G→A	A312V (GCC→GTC)	glcE ←	glycolate dehydrogenase, putative FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,588	0	G	A	100.0%	104.7 / NA	32	A312V (GCC→GTC)	glcE	glycolate dehydrogenase, putative FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/9);  total (23/9)

TTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCG  >  NC_000913/3125454‑3125723                                                                                                                                       |                                                                                                                                        ttGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTa                                                                                                                                    >  1:40343/1‑140 (MQ=255)    cATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAg                                                                                                                                 <  2:770466/140‑1 (MQ=255)         ccTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATACAGGAGCCGTTAGGGGGTCAAAGCCACCATCACCGACACTAAAGCGGGt                                                                                                                            >  2:371350/1‑140 (MQ=255)              tcctcAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCAt                                                                                                                       >  2:524220/1‑140 (MQ=255)                          cgcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGt                                                                                                           >  1:646333/1‑140 (MQ=255)                               aCATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                                                                                      >  2:176018/1‑140 (MQ=255)                                cATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                                                                                     <  2:40343/140‑1 (MQ=255)                                   gcgcCCGGCGTAAAACCAGCCGCAAGGGTCGGGGTCCTGTTTAAGCTGCGGGTGATAGCGAAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcga                                                                                                  <  2:381044/140‑1 (MQ=255)                                     gccgggggTAAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                                                                                                <  2:570434/136‑1 (MQ=255)                                      aCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                                                                                               >  1:570434/1‑140 (MQ=255)                                       ccGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg                                                                                              <  1:116907/140‑1 (MQ=255)                                       ccGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg                                                                                              <  1:86490/140‑1 (MQ=255)                                           ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                                                                                               <  1:550417/135‑1 (MQ=255)                                           ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                                                                                               >  2:550417/1‑135 (MQ=255)                                           ggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATgg                                                                                          >  2:643970/1‑140 (MQ=255)                                                     gccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGt                                                                                >  1:31618/1‑140 (MQ=255)                                                               tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCTATGCGACGGATTTCATTGTCCTCGGCTGt                                                                      >  1:298914/1‑140 (MQ=255)                                                               tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGt                                                                      >  2:408474/1‑140 (MQ=255)                                                                                ctgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTa                                                     <  1:408474/140‑1 (MQ=255)                                                                                    tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgc                                                 >  1:576399/1‑140 (MQ=255)                                                                                    tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAcgc                                                 >  1:595709/1‑140 (MQ=255)                                                                                      gTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATCGGCCTCGGCTGTAGATTTCAGCCAGCGTAAcgccc                                               >  2:363181/1‑140 (MQ=255)                                                                                                aaTAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTc                                     >  1:710138/1‑140 (MQ=255)                                                                                                     aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCa                                >  2:181930/1‑140 (MQ=255)                                                                                                          ccGATAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGACCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGc                           >  2:548411/1‑140 (MQ=255)                                                                                                            gATAGCGGGACAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTc                         >  1:733245/1‑140 (MQ=255)                                                                                                             aTAGCGGGGCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCg                        >  2:22863/1‑140 (MQ=255)                                                                                                              tgggggggCAAAGCCACCTCCGCCAAAGCTAAAGGGGGTGGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGc                       <  2:443568/136‑1 (MQ=255)                                                                                                                  ggggAAAAGCCCCCTTCCCCGACACTAAGGGGGGTGCCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCggg                   <  1:524220/140‑1 (MQ=255)                                                                                                                   gggCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGt                  <  1:181930/140‑1 (MQ=255)                                                                                                                    ggCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTa                 >  1:198705/1‑140 (MQ=255)                                                                                                                    ggCAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATGTCAGCCAGCGTATCGCCCCGCCCCACTCGAGCAGTTGCTCGCCGGGTa                 >  2:257040/1‑140 (MQ=255)                                                                                                                      cAAAGCCACCATCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTACCGCCCCGACCCTGTAGATCAGCTGCTCGCCGGGTaaa               >  1:402872/1‑140 (MQ=255)                                                                                                                            ccccaTCCCCGACACAAAAGGGGGTCGCATGCCCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatc         <  2:595709/138‑1 (MQ=255)                                                                                                                              ccaTCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatcat       <  1:643970/140‑1 (MQ=255)                                                                                                                              ccaTCTCCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatcat       <  2:198705/140‑1 (MQ=255)                                                                                                                              ccaTCTCCGACACTAAAGCGGGGCGCATGTCCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCcatcat       <  1:257040/140‑1 (MQ=255)                                                                                                                                   tcCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGgcg  >  1:565425/1‑140 (MQ=255)                                                                                                                                   tcCGACACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGgcg  <  2:733245/140‑1 (MQ=255)                                                                                                                                   tcCGACACTAAAGCGCGTCGCATGACCGCTAGCGTTGAGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGgcg  <  1:260948/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCG  >  NC_000913/3125454‑3125723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑