Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,148,203	T→C	W103R (TGG→CGG)	gpr →	L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,148,203	0	T	C	100.0%	96.9 / NA	29	W103R (TGG→CGG)	gpr	L‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

TTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCC  >  NC_000913/3148066‑3148330                                                                                                                                          |                                                                                                                                ttACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCgg                                                                                                                               >  2:756635/1‑140 (MQ=255)  tACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCggc                                                                                                                              >  1:659134/1‑140 (MQ=255)                   ccAACAATTACGGGACGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGctct                                                                                                             >  2:597297/1‑140 (MQ=255)                                  cgccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTTACGTaaa                                                                                              >  2:510612/1‑140 (MQ=255)                                    ccTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATa                                                                                            >  2:423682/1‑140 (MQ=255)                                     cTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATAc                                                                                           <  2:359808/140‑1 (MQ=255)                                        cAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTg                                                                                        <  2:659134/140‑1 (MQ=255)                                             aGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGc                                                                                   <  1:159458/140‑1 (MQ=255)                                               cgcAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTTTGGAGGATCACGTGAATACCTGCTCGCCa                                                                                 >  1:380431/1‑140 (MQ=255)                                               cgcAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCa                                                                                 >  1:544833/1‑140 (MQ=255)                                               cgcAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGCTGACGTAAATACCTGCTCGccc                                                                                 >  2:781571/1‑139 (MQ=255)                                                   aaagaGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCt                                                                             <  1:529842/139‑1 (MQ=255)                                                        gaACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGAcc                                                                        <  1:756635/140‑1 (MQ=255)                                                        gaACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGACTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGAcc                                                                        <  2:559737/140‑1 (MQ=255)                                                              tGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGcc                                                                  >  1:558675/1‑140 (MQ=255)                                                               ggTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCt                                                                 <  2:691668/140‑1 (MQ=255)                                                                       gctgcGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTa                                                         <  2:464124/140‑1 (MQ=255)                                                                       gctgcGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTa                                                         <  2:558675/140‑1 (MQ=255)                                                                            gggAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATgggg                                                    <  1:423682/140‑1 (MQ=255)                                                                            gggAGGA‑TTTGCCGCATATCGCTATCAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGc                                                   <  1:380456/140‑1 (MQ=255)                                                                              gaggaTTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCt                                                  <  2:544833/140‑1 (MQ=255)                                                                                   ttttGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGt                                             >  2:574255/1‑140 (MQ=255)                                                                                    tttGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTa                                            <  1:574255/140‑1 (MQ=255)                                                                                     ttGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTAt                                           <  1:781571/140‑1 (MQ=255)                                                                                           cTTATCGCGATGAACTACTTATCTCTACCATGGGTAGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGat                                     <  2:380431/140‑1 (MQ=255)                                                                                                              tATCTCTACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATcgcg                  <  1:135492/140‑1 (MQ=255)                                                                                                                    tACCAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGAAATCTTTTACTCTCATCGCGTCGATg            >  1:328592/1‑140 (MQ=255)                                                                                                                      ccAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGaa          >  1:722323/1‑140 (MQ=255)                                                                                                                      ccAAGGCTGGCTACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGAATGGGGCTAGAGTATGGCGATATCTGTTACTCTCATCGCGTCGATGaa          >  2:291936/1‑140 (MQ=255)                                                                                                                           gctggctACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATAc     >  2:568696/1‑140 (MQ=255)                                                                                                                              ggctACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCTATATCTTATACACTGATCGCGACGATGAAAATACGcc  >  1:385099/1‑140 (MQ=255)                                                                                                                              ggctACGATATGCGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATTGGGGCGATGaaaagaagac  >  2:760126/1‑134 (MQ=255)                                                                                                                                          |                                                                                                                                TTACGCACTTTGATTTAGCCAACAATTACGGGCCGCCTCCAGGAAGCGCAGAAGAGAACTTTGGTCGCCTGCTGCGGGAGGATTTTGCCGCTTATCGCGATGAACTGATTATCTCTACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCC  >  NC_000913/3148066‑3148330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑