| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,160,621 | A→G | S176P (TCC→CCC) | ygiQ ← | radical SAM superfamily protein YgiQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,160,621 | 0 | A | G | 97.4% | 129.7 / NA | 39 | S176P (TCC→CCC) | ygiQ | radical SAM superfamily protein YgiQ |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/23); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACATCGCGGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCG > NC_000913/3160486‑3160751 | gCACATCGCGGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAg < 1:463024/140‑1 (MQ=255) aTCGCGGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAAt > 1:471275/1‑140 (MQ=255) cgcgGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCa > 1:540183/1‑140 (MQ=255) cgcgGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCa > 1:523486/1‑140 (MQ=255) ttCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCCTAATgcg < 2:471275/140‑1 (MQ=255) ttCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATgcg < 1:744402/140‑1 (MQ=255) ttCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATgcg < 2:499690/140‑1 (MQ=255) tCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATgcg > 2:645298/1‑139 (MQ=255) tCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATgcg < 1:645298/139‑1 (MQ=255) gacacagCTCGCCCATCGCCAGTCGATGCGCTACTTCCACCAGCGGACCCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTa < 2:328519/134‑1 (MQ=255) ggCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGgcg < 2:540183/140‑1 (MQ=255) tCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGTTATCGGGCCAGCAAACATAATGCGCGGTACGGCGCAg > 1:396517/1‑140 (MQ=255) tCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAg > 1:492823/1‑140 (MQ=255) cgaccATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGa < 2:492823/137‑1 (MQ=255) cGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGa < 1:6060/140‑1 (MQ=255) cGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCAAAATGCGCGGTACGGCGCAGa < 1:595486/140‑1 (MQ=255) cccATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACt < 2:523486/140‑1 (MQ=255) ccAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCTGTAATCAGAATTCGGGGTACGCCGCAGACtctgtctc > 1:328519/1‑133 (MQ=255) gACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATa < 1:536344/140‑1 (MQ=255) aCGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCATCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATAc > 1:114553/1‑140 (MQ=255) tGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATAATAATGCGAGGTACGGCGCAGATTAGCCTCAATAccgcc > 2:387111/1‑140 (MQ=255) tGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATACATGGTACGGAGCAGTATAGCCTCAATAccgcc > 2:395798/1‑140 (MQ=255) aCTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGAt > 2:500334/1‑140 (MQ=255) gCGGACGCTCACCGTTACCAAAAATTAGCTTGTCGGCCTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACT‑GCCTCAATACCGCCGAGGATCACCGGTACAt < 1:374633/140‑1 (MQ=255) gCGGACGCCCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACa < 1:137505/140‑1 (MQ=255) cGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACAt > 2:590975/1‑140 (MQ=255) cGGACGCTCACCGTTACCAAACATCAGCATGCCGGCTTTTGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACAt < 2:331668/140‑1 (MQ=255) tCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCAAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCa < 1:69842/140‑1 (MQ=255) ccGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGc < 2:62776/140‑1 (MQ=255) aCCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCct > 2:749176/1‑137 (MQ=255) aCCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCct < 1:749176/137‑1 (MQ=255) cAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTa < 2:493650/140‑1 (MQ=255) tCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGc > 1:803494/1‑140 (MQ=255) cAGCATGTCGGCTTTCGAATCCCCCTGCATGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATCCCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt < 1:395798/140‑1 (MQ=255) cAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt < 1:590975/140‑1 (MQ=255) cAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt < 1:500334/140‑1 (MQ=255) cAGCATGCAGGCTTTCCAATCCTCCAGCAGGGAACAGCGGCCGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTACCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCt < 2:396517/140‑1 (MQ=255) tCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCATTCGACCCCTCTTTACAACGCTGGGtata > 1:331042/1‑140 (MQ=255) aGCACGGAACGGCGCCGCGTACGGGGCCAGTCATCGTAAGGCGCGGTCCGGCGCAGACTAGCCTCAATCCCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATc < 2:331042/140‑1 (MQ=255) aCGGAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGGTGGATATAAACCACTGTGGCGCGATCCgg > 1:353282/1‑140 (MQ=255) gAACGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGgcg > 1:602516/1‑140 (MQ=255) aCGGCGCACGGTATCGGGCCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCt < 2:803494/140‑1 (MQ=255) cGGTATCGGGCCAGTAAGCATAGTGCGCGGTGCCGGGCATACTAGCCTTAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCcgcg < 2:353282/140‑1 (MQ=255) | GCACATCGCGGATTTCACTAATTGGCTCGCCCATCGCCAGACGATGCGCCACTTCCACCAGCGGACGCTCACCGTTACCAAACATCAGCATGTCGGCTTTCGAATCCACCAGCACGGAACGGCGCACGGTATCGGACCAGTAATCATAATGCGCGGTACGGCGCAGACTAGCCTCAATACCGCCGAGGATCACCGGTACATCTTTCCACGCCTCTTTACAACGCTGGGTATAAACCAGTGTGGCGCGATCCGGGCGCTTACCCGCG > NC_000913/3160486‑3160751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |