Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,795	T→C	Y258H (TAC→CAC)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,795	0	T	C	100.0%	47.3 / NA	15	Y258H (TAC→CAC)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/0);  total (15/0)

GCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGG  >  NC_000913/3208671‑3208923                                                                                                                             |                                                                                                                                 gCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGa                                                                                                                   >  1:728269/1‑140 (MQ=255)        ttgggACTGATGAAAGGCCCATCCCCCGCGGGGGGGGGTGGGGGCGTGTGGTTCCGGGGGTTGTCGCCGCTCAGTTTTTTTTTGGTTGTGCTGGTTCCGTGGTGGGCTTCCGTGCTCCCCCCGCCGGTACTGAAGTCTgg                                                                                                            <  2:156113/137‑1 (MQ=255)                 atgaAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGt                                                                                                   >  1:114993/1‑140 (MQ=255)                 atgaAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGt                                                                                                   >  1:156113/1‑140 (MQ=255)                 atgaAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGt                                                                                                   >  1:785983/1‑140 (MQ=255)                                                      aCTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc                                                              >  1:429798/1‑140 (MQ=255)                                                      aCTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc                                                              >  2:553668/1‑140 (MQ=255)                                                                   gATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTcgc                                                 >  1:561689/1‑140 (MQ=255)                                                                    aTGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGACCTGCAGGCAATGGACCCGCTCTGTTcgcg                                                >  1:346650/1‑140 (MQ=255)                                                                             cTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACg                                       >  2:738936/1‑140 (MQ=255)                                                                             cTCAGCATTTTACTGGTTCCGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACg                                       >  1:555508/1‑140 (MQ=255)                                                                                          tGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTg                          >  1:791541/1‑140 (MQ=255)                                                                                            gTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGAt                        >  1:23360/1‑140 (MQ=255)                                                                                               ctgctgGTTCCCTGGCTGGCTTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGTAATGGGCCGGCTGTGTTCGCGTGAAAAACGTATGCTGGGGCTGATGGt                     >  2:258357/1‑140 (MQ=255)                                                                                                            ggctggctTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGTGCTGATGGTAGGCGCGCTGGTg        >  1:331892/1‑140 (MQ=255)                                                                                                                  ctTACGTGCTGCACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTgg  >  2:70124/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                 GCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTAGGCGCGCTGGTGCTGTGG  >  NC_000913/3208671‑3208923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑