Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,945	T→C	A334A (GCT→GCC)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,945	0	T	C	100.0%	108.0 / NA	31	A334A (GCT→GCC)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/15);  total (16/15)

CCGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATT  >  NC_000913/3240809‑3241079                                                                                                                                         |                                                                                                                                       ccGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCctct                                                                                                                                     <  1:425716/140‑1 (MQ=255) ccGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCctct                                                                                                                                     <  1:5097/140‑1 (MQ=255)                              aaTATGGCGGGCGCAGCAATCACTAATACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGc                                                                                                        <  1:296701/140‑1 (MQ=255)                              aaTATGGCGGGCGCAGCAAACACTATTATCGCGTTGTCGCGCGCGGGGGGGAAAATGCTGGGGGTTTCGGTCGAACCGCCCCCCGCGCCGCCGGTGGGTGGGGGGGGCTCTTTTTTTGCCTGTGGCGCGTCCGGCGGGGc                                                                                                        >  2:627219/1‑140 (MQ=255)                                      gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                <  2:30145/140‑1 (MQ=255)                                      gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                <  1:773673/140‑1 (MQ=255)                                         cgcAGCAATCACTATTACCGTGATGACGCTGCCTGCGGTTAATACGCTCGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGtctc                                                                                             <  2:229546/140‑1 (MQ=255)                                           cagcaATCACTATTACCGTGTTGACACTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctg                                                                                           <  1:305544/140‑1 (MQ=255)                                                       ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCa                                                                               <  1:627219/140‑1 (MQ=255)                                                        tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCAGGCGGGGCTATGCTGCTGATCCCAc                                                                              >  1:384859/1‑140 (MQ=255)                                                         gccGTGTTGACGCTGGCTGCGGCTAATACGCTGTGTATTCCGGTCGATGTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCAGCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCTCACt                                                                             <  2:354594/139‑1 (MQ=255)                                                         aCCGTGTTGACGCTGTCTGCGGCTAATACCCTGGGTACTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACt                                                                             <  1:401249/140‑1 (MQ=255)                                                         aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACt                                                                             <  2:821684/140‑1 (MQ=255)                                                           cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                           <  2:3083/140‑1 (MQ=255)                                                            gtgtTGACTCTGGCTGCGGTTAATACGCTGGTTATCCCGTTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                          <  2:384859/140‑1 (MQ=255)                                                            gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                          <  1:527318/140‑1 (MQ=255)                                                            gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGc                                                                          >  2:527318/1‑140 (MQ=255)                                                             tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGTCTGCTGCTGATCCCAcccggc                                                                         >  2:467940/1‑135 (MQ=255)                                                               ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGTGGGGCTGTGCTGCGGATCCCACTGGCCTg                                                                       >  1:243302/1‑140 (MQ=255)                                                                 gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                     >  2:538393/1‑140 (MQ=255)                                                                 gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCACTACTGCTGCTCCCACTGGCCTGTa                                                                     >  1:320731/1‑140 (MQ=255)                                                                     ctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAatat                                                                 <  1:22978/140‑1 (MQ=255)                                                                       ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCAGCGTGGCGGGGGTGGCTCTGGTGCTAATCCGCCTCGCCTGTAATATGt                                                               >  2:275740/1‑140 (MQ=255)                                                                         ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                             >  1:825452/1‑140 (MQ=255)                                                                             ggTTAATACGCTGGGTATTCCGCTCGATCTGCCCAGGGCGCTGCTGTTCAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTa                                                         <  2:320731/140‑1 (MQ=255)                                                                                  aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCg                                                    >  1:687913/1‑140 (MQ=255)                                                                                      gCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTCGGGATTTTGAACg                                                >  1:26763/1‑140 (MQ=255)                                                                                          ggTATTCCGGTCGATCTGCCCATGGCGCTGCTGTTGATCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                            <  1:254347/140‑1 (MQ=255)                                                                                                              cACGGCGCTGCTGTTGAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                        <  1:625239/140‑1 (MQ=255)                                                                                                                             gAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg         >  1:619336/1‑140 (MQ=255)                                                                                                                             gAGCGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg         >  1:11007/1‑140 (MQ=255)                                                                                                                                cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg      >  2:660768/1‑140 (MQ=255)                                                                                                                                cGTGGTGGCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTTCTGCTGATCCCCCTGGCCTGTAATATGTTTGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg      >  2:445802/1‑140 (MQ=255)                                                                                                                                   ggtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTTGAACGGTATCGCCATGCAGGGGGTTGCCGTCGGCCTTTTTATCTGCGTAt   >  2:206309/1‑140 (MQ=255)                                                                                                                                    gtggCCTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCCGCCGATCCCACCTGCCTGTAATATGTTTGGTAATTCGAACCATATTCCCACTCAGGGGGGTGCCCGCGGCTTTATCCTCGGCGTAtt  >  2:741379/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATT  >  NC_000913/3240809‑3241079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑