Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,342	T→C	L40S (TTA→TCA)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,342	0	T	C	98.8%	273.0 / NA	81	L40S (TTA→TCA)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (37/42);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGATGAGGTTTTACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGT  >  NC_000913/3245208‑3245476                                                                                                                                       |                                                                                                                                       aaCGATGAGGTTTTACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACa                                                                                                                                   >  1:481645/1‑140 (MQ=255)  aCGATGAGGTTTTACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACAt                                                                                                                                  >  2:77194/1‑140 (MQ=255)      tGAGGTTTTACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTcc                                                                                                                              >  1:519453/1‑140 (MQ=255)          gTTTTACATGCGTAAGATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCAccc                                                                                                                          <  1:117239/140‑1 (MQ=255)          gTTTTACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCAccc                                                                                                                          <  1:77194/140‑1 (MQ=255)          gTTTTACATGCGTAAAAGTAAACGGTTACGTTGGTGTATCATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCAccc                                                                                                                          <  2:287782/140‑1 (MQ=255)          gTTTTACAGGCGTAAAATTAAAGGGTTACGTAGTTATATGATCGCACTCGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCAccc                                                                                                                          <  2:379934/140‑1 (MQ=255)            tttACATGCGTAAAATTAAAGGTTTACGTTGTTATCTGACCGCACTGGCGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCaa                                                                                                                        <  2:219779/140‑1 (MQ=255)            tttACATGCGTAAAATTAAAGGCTTACGTTGATATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCaa                                                                                                                        <  2:296167/140‑1 (MQ=255)             ttACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTGACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAAc                                                                                                                       <  1:642873/140‑1 (MQ=255)             ttACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTACGCCAACGCTGATGGATGAGTTAAACATCTCCACCCAAc                                                                                                                       >  2:272479/1‑140 (MQ=255)              tACATGCGTAAAATTTAAGGGTTACGTTGGTATATGAGCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACa                                                                                                                      <  1:320956/140‑1 (MQ=255)              tACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACa                                                                                                                      <  1:450815/140‑1 (MQ=255)               aCATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAg                                                                                                                     <  1:654217/140‑1 (MQ=255)                    cGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTTTGATGGAGGAGGCAAACATCTCCACCCAACAGTATTc                                                                                                                >  1:287782/1‑140 (MQ=255)                    cGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTAt                                                                                                                  >  2:85035/1‑138 (MQ=255)                    cGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTAt                                                                                                                  <  1:85035/138‑1 (MQ=255)                    cGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTc                                                                                                                >  1:92462/1‑140 (MQ=255)                    cGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTc                                                                                                                >  1:745403/1‑140 (MQ=255)                    cGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTc                                                                                                                >  1:629095/1‑140 (MQ=255)                         aaTTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAg                                                                                                                     >  2:686997/1‑130 (MQ=255)                         aaTTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAg                                                                                                                     <  1:686997/130‑1 (MQ=255)                             aaaGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACa                                                                                                                      <  1:480842/125‑1 (MQ=255)                             aaaGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACa                                                                                                                      >  2:480842/1‑125 (MQ=255)                              aaGGGTTACGTTGGTTTATGATGGCTCTGCTGACGCTGGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATatcatc                                                                                                      <  1:246778/140‑1 (MQ=255)                                    tACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCGAACATCTCCACCCAACAGTATCTCTATATCCTCGCAGcc                                                                                                >  2:395576/1‑140 (MQ=255)                                    tACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAGCATCTCCACCCGACAGTATTCCTATATCATCGCAGcc                                                                                                >  1:247366/1‑140 (MQ=255)                                     aCGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCt                                                                                               >  2:810332/1‑140 (MQ=255)                                      cGTTGGTATATGATCCCACTGGAGAAGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTa                                                                                              <  1:315500/140‑1 (MQ=255)                                        ttGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTAtt                                                                                            <  2:745403/140‑1 (MQ=255)                                           gTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctg                                                                                         <  2:481645/140‑1 (MQ=255)                                           gTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctg                                                                                         >  1:793258/1‑140 (MQ=255)                                             atatGATCGCACTGGTGTCGCTCGGCACCGTTCTTTGTTACCTGACGCCTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctgct                                                                                       <  2:247366/140‑1 (MQ=255)                                                tGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCGACAGTATGCCTGTAGCATCGCAGCCTATTCTgctgct                                                                                    >  1:219502/1‑140 (MQ=255)                                                     gCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTGCACCCAACAGTAATCCGATATCATCGCAGCGTATTCTGCTGCTTATAc                                                                               >  2:210207/1‑140 (MQ=255)                                                     gCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATAc                                                                               >  2:657323/1‑140 (MQ=255)                                                     gCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGGATTCCTAGATCATCGCAGCCTGTTCTGCTGCTTATAc                                                                               >  1:255361/1‑140 (MQ=255)                                                     gCACTGGTGACGCTCCGCACCGAGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATAc                                                                               <  1:395576/140‑1 (MQ=255)                                                      cACTGGTGACGCTCGGCACGGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATGCCTTTATCATCGCAGCCCATTCTGCTGCTTATACg                                                                              >  2:320521/1‑140 (MQ=255)                                                      cACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                                                                              >  2:477986/1‑140 (MQ=255)                                                      cACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                                                                              >  2:24997/1‑140 (MQ=255)                                                      cACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                                                                              >  1:12262/1‑140 (MQ=255)                                                      cACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                                                                              >  1:431652/1‑140 (MQ=255)                                                          ggTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCa                                                                          >  2:657197/1‑140 (MQ=255)                                                          ggTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCACCGCAGCGTATTCTTCTGCTTATACGGTCa                                                                          >  1:361305/1‑140 (MQ=255)                                                           gTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTGCTACATCATCGCAGCCTATTCAGCTGCTTATACGGTCAt                                                                         >  2:290827/1‑140 (MQ=255)                                                            tGACGCTCCGCACCGTTCTTGGGTACCTGACTCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATg                                                                        <  2:361305/140‑1 (MQ=255)                                                                     gCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTa                                                               >  1:136080/1‑140 (MQ=255)                                                                       aCCGTGCTTGGTTACCTGACGCTTAACACTGAGGCTGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc                                                             <  2:255361/140‑1 (MQ=255)                                                                       aCCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc                                                             <  1:477986/140‑1 (MQ=255)                                                                       aCCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc                                                             <  2:793258/140‑1 (MQ=255)                                                                           tGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATGATCGCAGCCTATGCTGGTGCTTATAGGGTCGTGCAACCGGTAGCAGGt                                                         >  2:230022/1‑140 (MQ=255)                                                                             cTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa                                                       <  1:545511/140‑1 (MQ=255)                                                                             cTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa                                                       <  1:123096/140‑1 (MQ=255)                                                                                ggTTACCTGACGCGTAACACTGAGGCGGCAGCTGCTCCACCTCTGATGCAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGt                                                    <  2:219502/140‑1 (MQ=255)                                                                                 gTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTa                                                   <  2:623287/140‑1 (MQ=255)                                                                                  ttACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTAc                                                  <  1:810332/140‑1 (MQ=255)                                                                                     ccTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTgg                                               >  1:694676/1‑140 (MQ=255)                                                                                       tGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc                                                             >  2:828473/1‑124 (MQ=255)                                                                                       tGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc                                                             <  1:828473/124‑1 (MQ=255)                                                                                       tGACGCGTAACACTGCGGCGGCGGCCGCGCCAACTCTCATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGAt                                             <  1:272479/140‑1 (MQ=255)                                                                                            cgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCt                                        >  1:137346/1‑140 (MQ=255)                                                                                            cgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCt                                        >  1:791620/1‑140 (MQ=255)                                                                                              tAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGATCAGGCAGCCGGTAGCCGGTTGTGTACTGGATGTGCTgg                                      >  2:284728/1‑140 (MQ=255)                                                                                                 cacTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTa                                   <  2:431652/140‑1 (MQ=255)                                                                                                   cTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACg                                 >  2:21430/1‑140 (MQ=255)                                                                                                      tgGCGGCAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGaaa                              >  2:54634/1‑140 (MQ=255)                                                                                                       ggcggcAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGaaaa                             >  1:20937/1‑140 (MQ=255)                                                                                                       ggcggcAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGaaaa                             >  2:173600/1‑140 (MQ=255)                                                                                                        gcggcAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAAt                            <  1:173600/140‑1 (MQ=255)                                                                                                        gcggcAGCTGCGCCAACTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAAt                            <  2:12262/140‑1 (MQ=255)                                                                                                                    ccAACTCTGATGGAATAGTCAACCACCTCTACCCAACTGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAAt                <  1:290827/140‑1 (MQ=255)                                                                                                                      aaCTCTGATGGAAGAGTCAAACTTCTCCATCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGt              <  1:284728/140‑1 (MQ=255)                                                                                                                      aaCTCTGATGGAAGAGTCAAACGTCTCCACCCAACGGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGt              <  2:282777/140‑1 (MQ=255)                                                                                                                      aaCTCTGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGt              <  1:657323/140‑1 (MQ=255)                                                                                                                      aaCTCTGATGGAAGAGTAAAACCTCTCCACCCAACTGTAATCCTATATTATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGt              <  2:210545/140‑1 (MQ=255)                                                                                                                           tGATGGAAGAGTCAATCATCTCCACCCAATAGTATTCTTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTtgct         <  1:320521/140‑1 (MQ=255)                                                                                                                           tGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCTGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTtgct         <  1:21430/140‑1 (MQ=255)                                                                                                                           tGATGGAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTtgct         <  2:629095/140‑1 (MQ=255)                                                                                                                               ggAAGAGTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTtgctgtgc     <  2:694676/140‑1 (MQ=255)                                                                                                                                  agagTCAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTgctgtgctgt  <  2:136080/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AACGATGAGGTTTTACATGCGTAAAATTAAAGGGTTACGTTGGTATATGATCGCACTGGTGACGCTCGGCACCGTGCTTGGTTACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGT  >  NC_000913/3245208‑3245476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑