Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,267,000	A→G	G22G (GGT→GGC)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,267,000	0	A	G	100.0%	191.4 / NA	55	G22G (GGT→GGC)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/29);  total (26/29)

GACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGAC  >  NC_000913/3266864‑3267136                                                                                                                                         |                                                                                                                                         gACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCa                                                                                                                                       <  1:538289/140‑1 (MQ=255)  aCCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCCCCACTAATTCCACACCAAAAAAATCTTCAATTTCGTTAATGATTTTACTGCCGGCCGGTTGATTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCAc                                                                                                                                      <  1:110934/140‑1 (MQ=255)  aCCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCAc                                                                                                                                      >  1:540684/1‑140 (MQ=255)         ggTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAAt                                                                                                                               <  2:190447/140‑1 (MQ=255)              tAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACtt                                                                                                                          <  1:605033/140‑1 (MQ=255)                     acacCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCACCCGAGCCGATAGAGCCACTTCCAATGACTTCCTGaaa                                                                                                                   >  1:299199/1‑140 (MQ=255)                           gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTAc                                                                                                             <  2:663099/140‑1 (MQ=255)                                     gCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGtgctgc                                                                                                   >  1:460055/1‑140 (MQ=255)                                      caccacTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCTCTTCTAAAGACGTCATGAAAGTCTACCAGGTGCTGCg                                                                                                  >  1:376606/1‑140 (MQ=255)                                      caccacTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCg                                                                                                  >  2:196361/1‑140 (MQ=255)                                      caccacTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGACGATAGAGACACTTGTAATGACTTCATGAACGACTACCAGGTGCTGCg                                                                                                  >  2:365989/1‑140 (MQ=255)                                           cTAATTCCACACCAAGATAATCATCTATAGCGTTAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTc                                                                                             <  1:275769/140‑1 (MQ=255)                                             aaTTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                                                                           <  1:40303/140‑1 (MQ=255)                                             aaTTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                                                                           <  2:540684/140‑1 (MQ=255)                                                tCCACACCAAAATAATCTTCAATATTGTTTATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaag                                                                                        <  1:365989/140‑1 (MQ=255)                                                   acacCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaag                                                                                     >  1:620560/1‑140 (MQ=255)                                                    cacCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaaga                                                                                    >  1:614314/1‑140 (MQ=255)                                                     cccAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCGGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaagaa                                                                                   <  1:136866/139‑1 (MQ=255)                                                     cccAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGgaagaagaa                                                                                   <  1:131327/139‑1 (MQ=255)                                                     acCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                                                                           <  1:166364/132‑1 (MQ=255)                                                     acCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                                                                           >  2:166364/1‑132 (MQ=255)                                                         aaaTAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGt                                                                               >  1:823220/1‑140 (MQ=255)                                                          aataatCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTg                                                                              >  2:48150/1‑140 (MQ=255)                                                          aataatCTTAAATTTCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTCCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTg                                                                              <  2:519594/140‑1 (MQ=255)                                                                    aaTATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGGGTTTTCGGAAGAAGAATAGTGCTCATAACGa                                                                    >  2:377196/1‑140 (MQ=255)                                                                      tatCGTTAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGc                                                                             <  1:447981/129‑1 (MQ=255)                                                                      tatCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGc                                                                             >  2:447981/1‑129 (MQ=255)                                                                        tCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTa                                                                <  2:738342/140‑1 (MQ=255)                                                                           ttAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCt                                                                            <  1:433516/125‑1 (MQ=255)                                                                           ttAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCt                                                                            >  2:433516/1‑125 (MQ=255)                                                                           ttAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGt                                                             >  1:195355/1‑140 (MQ=255)                                                                                   tttACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCAGAAGAAGAATAGTGCTAATAAAGACCTACGTTAATTAcc                                                     >  2:310497/1‑140 (MQ=255)                                                                                           cGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGAc                                             >  2:118455/1‑140 (MQ=255)                                                                                            ggCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACg                                            >  2:528850/1‑140 (MQ=255)                                                                                            ggCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACg                                            >  1:372220/1‑140 (MQ=255)                                                                                               cGGTTGATGTACCCCTAATTCTGTTGCAGCGTAGCCGATGGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCa                                         <  2:376606/140‑1 (MQ=255)                                                                                               cGGTTGAGTTACCCCTTATTCTTTTGCAGCCGACCTGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCa                                         <  2:372220/140‑1 (MQ=255)                                                                                               cGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCa                                         >  1:460950/1‑140 (MQ=255)                                                                                               cGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCa                                         <  2:620560/140‑1 (MQ=255)                                                                                                   tGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGaa                                     <  1:118455/140‑1 (MQ=255)                                                                                                       ttAACCCTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACATTACTTACCTCATAGACGGCATGAAgtgt                                 >  1:273515/1‑140 (MQ=255)                                                                                                             cTAATTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCaaa                           <  1:196361/140‑1 (MQ=255)                                                                                                               aaTTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAaaat                         >  1:499893/1‑140 (MQ=255)                                                                                                               aaTTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACCTTAATTAACTCGTTAACGGCAAGAAGTGTATCAaaat                         >  1:254930/1‑140 (MQ=255)                                                                                                                aTTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCaaa                           >  2:446909/1‑137 (MQ=255)                                                                                                                aTTCTTTTGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCaaa                           <  1:446909/137‑1 (MQ=255)                                                                                                                     tttGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAaaatgaaatg                   <  2:460950/140‑1 (MQ=255)                                                                                                                      ttGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAaatgaaatga                  >  2:425030/1‑140 (MQ=255)                                                                                                                       tGCAGCCGAGCCGATAGAGCCACTTCTAATGTCTTGCTGGAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                 <  2:273515/140‑1 (MQ=255)                                                                                                                       tGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                 <  2:460055/140‑1 (MQ=255)                                                                                                                       tGCAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGAGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                 <  2:499893/140‑1 (MQ=255)                                                                                                                       tGCAGCCGAGCCGATAACGCCACTTCTAATGACTTACTGTAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                 <  2:363733/140‑1 (MQ=255)                                                                                                                         cAGCCGAGCCGATAGAGCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACa               >  1:173857/1‑140 (MQ=255)                                                                                                                           gccgagccgaTAGAGCCCCTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAgg             <  2:299199/140‑1 (MQ=255)                                                                                                                           ctcgagccgaTAGAGCCACTTCTAATGACTTCCTGAAAGACCACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAACTACGTTAATAACCTCATTGACGGCATGAAGTGTATCAAAATCAAATGAACAgg             <  2:823220/138‑1 (MQ=255)                                                                                                                                      agagCCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGAc  <  1:48150/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGAC  >  NC_000913/3266864‑3267136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑