Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,272,178	T→C	T192A (ACT→GCT)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,272,178	0	T	C	100.0%	161.6 / NA	46	T192A (ACT→GCT)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

TGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATC  >  NC_000913/3272045‑3272310                                                                                                                                      |                                                                                                                                     tGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCTAATTGCCTGATACAGCAAGTCCGGGGTAACGCCCGCTTTAGCTGCCAg                                                                                                                                >  2:383116/1‑140 (MQ=255)  gCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGc                                                                                                                               >  1:187191/1‑140 (MQ=255)     gATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCTGGTCAACGCCCGCGTTAGCTGCCAGCGtt                                                                                                                            >  1:339781/1‑140 (MQ=255)      aTCAATCCGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTa                                                                                                                           >  2:660313/1‑140 (MQ=255)      aTCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACGAGGTCCGTGTTAACGCCCGCTTTAGCTGCCAGCGTTa                                                                                                                           >  1:313964/1‑140 (MQ=255)       tCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGTCTGATAAATCAGGTCCAGGTTAACGACCGCTTTAGCTGCCAGCGTTaa                                                                                                                          >  2:213138/1‑140 (MQ=255)       tCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTaa                                                                                                                          >  1:443336/1‑140 (MQ=255)       tCAATACGGAAGCCCAGCTTGACGTTGCGCTCCATCCACATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTaa                                                                                                                          <  1:213138/140‑1 (MQ=255)        cAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAAc                                                                                                                         <  1:533451/140‑1 (MQ=255)                aaGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGa                                                                                                                 >  2:644180/1‑140 (MQ=255)                  gCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAACTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACa                                                                                                               >  2:603130/1‑140 (MQ=255)                   cccGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCCCTTTAGCTCCCAGCGTTAACGCTTCTGACAt                                                                                                              >  2:344256/1‑140 (MQ=255)                     cGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATcg                                                                                                            >  2:192956/1‑140 (MQ=255)                     cGGCTTGAAGTTGCGATCCATCGCCGTCGTCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATcg                                                                                                            <  1:383116/140‑1 (MQ=255)                        cTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCgg                                                                                                         <  2:443336/140‑1 (MQ=255)                                 gCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCa                                                                                                >  2:580312/1‑140 (MQ=255)                                  cGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTGAACGCTACTGACAGCGCGGCAATATTCAg                                                                                               >  1:214559/1‑140 (MQ=255)                                    gTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAgcg                                                                                             <  2:4391/140‑1 (MQ=255)                                         tCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCcaca                                                                                        <  1:131635/140‑1 (MQ=255)                                             cATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGa                                                                                    <  1:678503/140‑1 (MQ=255)                                             cATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGa                                                                                    >  1:33707/1‑140 (MQ=255)                                             cATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGa                                                                                    <  1:813781/140‑1 (MQ=255)                                               tCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGAcc                                                                                  >  2:440833/1‑140 (MQ=255)                                               tCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGAcc                                                                                  >  2:508307/1‑140 (MQ=255)                                               tCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGAcc                                                                                  >  2:754535/1‑140 (MQ=255)                                                       ttGGCATCCAGCACGGTTCTGCCCGCCAGTCGACCTCGATTTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGc                                                                          <  2:339781/140‑1 (MQ=255)                                                       ttGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGc                                                                          <  1:508307/140‑1 (MQ=255)                                                            aTCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGtt                                                                     <  1:489314/140‑1 (MQ=255)                                                                aGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGt                                                                 <  1:724270/140‑1 (MQ=255)                                                                   aCGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGAc                                                              <  1:192956/140‑1 (MQ=255)                                                                          tGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCt                                                       >  2:547042/1‑140 (MQ=255)                                                                            cccGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGc                                                     >  2:181667/1‑140 (MQ=255)                                                                            cccGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGc                                                     >  1:194471/1‑140 (MQ=255)                                                                                ccAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGAATTGCCAGTTTGGTGACGTTACCTGCACCg                                                 >  1:58008/1‑140 (MQ=255)                                                                                              aTTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATg                                   <  1:733880/140‑1 (MQ=255)                                                                                                   cTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGcacca                              <  2:187191/140‑1 (MQ=255)                                                                                                          aCCAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAAcc                       <  1:754535/140‑1 (MQ=255)                                                                                                            cAGGTCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCg                     >  1:468079/1‑140 (MQ=255)                                                                                                                tCCGGGTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCAt                 >  2:563797/1‑140 (MQ=255)                                                                                                                    ggTTAACGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCt             >  1:71922/1‑140 (MQ=255)                                                                                                                        aaCGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtca         <  2:468079/140‑1 (MQ=255)                                                                                                                        aaCGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGAATTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtca         <  2:58008/140‑1 (MQ=255)                                                                                                                         aCGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtcat        <  1:208473/140‑1 (MQ=255)                                                                                                                         aCGCCCGCTTTAGCTGCAAGCGCTAACGCTTCAGAAATCCCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtcat        <  2:313964/140‑1 (MQ=255)                                                                                                                          cGCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtcatc       <  1:440833/140‑1 (MQ=255)                                                                                                                           gCCCGCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCTACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtcatca      <  1:603130/140‑1 (MQ=255)                                                                                                                           gCCCGCTTTAGCTGCCAACGTTTACGCTTCTCACATCGCTGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTtcatca      <  2:214559/140‑1 (MQ=255)                                                                                                                               gCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATc  <  1:644180/140‑1 (MQ=255)                                                                                                                               gCTTTAGCTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATc  <  1:130262/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     TGCAGATCAATACGGAAGCCCGGCTTGAAGTTGCGGTCCATCACCATCGGCGCTTTGGCATCCAGCACGGTACTGCCCGCCAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATC  >  NC_000913/3272045‑3272310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑