Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,298,828	T→C	T7A (ACA→GCA)	yraR ←	putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,828	0	T	C	100.0%	189.9 / NA	55	T7A (ACA→GCA)	yraR	putative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/34);  total (21/34)

AGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAA  >  NC_000913/3298692‑3298961                                                                                                                                         |                                                                                                                                      aGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAAGGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAAt                                                                                                                                    <  2:818342/140‑1 (MQ=255) aGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAAt                                                                                                                                    <  2:814325/140‑1 (MQ=255)        gATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTAcc                                                                                                                             >  1:123152/1‑140 (MQ=255)           cATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGa                                                                                                                          >  1:645444/1‑140 (MQ=255)             tGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACt                                                                                                                        >  1:527617/1‑140 (MQ=255)             tGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACt                                                                                                                        >  2:408968/1‑140 (MQ=255)             tGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACt                                                                                                                        >  2:156007/1‑140 (MQ=255)                   ttAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAGCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCtt                                                                                                                  >  2:324182/1‑140 (MQ=255)                   ttAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCtt                                                                                                                  <  2:641115/140‑1 (MQ=255)                     aaaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCg                                                                                                                <  2:563466/140‑1 (MQ=255)                      aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACGGCCCACCATCCCCGTTACGCCTGCAATCAGTACCTGACTCATCTTCGc                                                                                                               >  2:348558/1‑140 (MQ=255)                      aaCACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGc                                                                                                               >  2:740600/1‑140 (MQ=255)                        cacCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTc                                                                                                             <  2:679503/140‑1 (MQ=255)                           cccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCCTTGGGACCGCAATCAGCACCTGACTCATCTTCGCTCCtt                                                                                                          >  2:376752/1‑140 (MQ=255)                            ccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCttt                                                                                                         >  1:3335/1‑140 (MQ=255)                             cGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTa                                                                                                        >  1:514576/1‑140 (MQ=255)                                gCATATCGCCCAACGTACGTCGTGTTGGCACAGCAATTGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAg                                                                                                     <  1:308288/140‑1 (MQ=255)                                gCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGGCCCGTTGCGCCTGCTATTAGTACCTGACTCATCTTCGCTGCTTTACAg                                                                                                     >  2:378786/1‑140 (MQ=255)                                        cccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCt                                                                                             <  2:492841/140‑1 (MQ=255)                                              ggACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                                                                                       <  1:199997/140‑1 (MQ=255)                                              ggACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                                                                                       <  1:197080/140‑1 (MQ=255)                                               gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                                                                      <  1:103479/140‑1 (MQ=255)                                                  gtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATGAGTACCTGGCTCGTCTGCGCTCCTTGACAGAATTGTCTGCCTTGCGCt                                                                                   >  2:212286/1‑140 (MQ=255)                                                  gtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACGCATCTTCGCT‑CGTTACAGAATGGTCTGCCTTGCGCTc                                                                                  >  1:220037/1‑140 (MQ=255)                                                      cgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                                                                               <  2:123152/140‑1 (MQ=255)                                                       gtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                                                                                       >  1:442351/1‑131 (MQ=255)                                                       gtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTg                                                                                       <  2:442351/131‑1 (MQ=255)                                                            cgcAGCAATGGCGTTGACTTTCGGTCCGTAAATCAACGTCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                                                                         <  1:313414/140‑1 (MQ=255)                                                            cgcAGCAATGGCGTTGACTTTCAGTGCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                                                                         <  1:376752/140‑1 (MQ=255)                                                                gcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCa                                                                     <  1:811030/140‑1 (MQ=255)                                                                gcaATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCa                                                                     <  2:527617/140‑1 (MQ=255)                                                                  aaTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc                                                                   <  1:610046/140‑1 (MQ=255)                                                                  aaTGGCGTTAACTTTCGCTTCGTTAATCAACTTCCGCAGTAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc                                                                   <  2:246931/140‑1 (MQ=255)                                                                  aaTGGCGTTAACTTTAGGTTCGTTACTCATCATCCGCGGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc                                                                   <  1:340968/140‑1 (MQ=255)                                                                     ggCGTTAACTTTCGGTTCGTTAATCAGCATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAgg                                                                <  1:324182/140‑1 (MQ=255)                                                                     ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAgg                                                                <  2:815549/140‑1 (MQ=255)                                                                         ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc                                                                   >  2:557618/1‑133 (MQ=255)                                                                         ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCAcc                                                                   <  1:557618/133‑1 (MQ=255)                                                                         ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTa                                                            >  1:112894/1‑140 (MQ=255)                                                                         ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGACTCATGGACCAGGCTTa                                                            >  1:792120/1‑140 (MQ=255)                                                                              tttCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGa                                                       <  2:435329/140‑1 (MQ=255)                                                                               ttCGATTCAGTAATCAGTATCCTCAGCAGGTCAACCCCCACCAGCCCCCTTGCGCCTGCAAGCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCGTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGAc                                                      <  2:364914/140‑1 (MQ=255)                                                                                tCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGAcc                                                     >  2:641136/1‑140 (MQ=255)                                                                                 cGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCa                                                    <  1:151025/140‑1 (MQ=255)                                                                                 cGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGAGCTCCACGGCTCATGCACCAGGCTTAATAGACCa                                                    <  2:632278/140‑1 (MQ=255)                                                                                    ttCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGa                                                 <  2:645444/140‑1 (MQ=255)                                                                                         tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTaa                                            <  2:112894/140‑1 (MQ=255)                                                                                         tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTaa                                            <  2:792120/140‑1 (MQ=255)                                                                                                        gcagGTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATtgtg                             <  2:3335/140‑1 (MQ=255)                                                                                                            gTGACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGaa                         >  2:131382/1‑140 (MQ=255)                                                                                                              gACCGCCCACCAGCCCCGTTGCGCCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAAt                       <  1:156007/140‑1 (MQ=255)                                                                                                                      accaGCCCCGTTGCGACTGCAACCACTACCTGACTCAGCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCa               <  1:212286/140‑1 (MQ=255)                                                                                                                               gTTGCGCCTGCAATCAGTACCTAACCCATGTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGca      <  1:348558/140‑1 (MQ=255)                                                                                                                                  gcgcCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCTCCGTTTGTGGGACATTCCCCTCATCCAATGcaaca   >  2:380363/1‑140 (MQ=255)                                                                                                                                   cgcCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGcaacaa  <  1:641136/140‑1 (MQ=255)                                                                                                                                   cgcCTGCAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGcaacaa  <  1:408968/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAA  >  NC_000913/3298692‑3298961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑